Efficient shoot regeneration of Brassica campestris using cotyledon explants cultured in vitro

1991 ◽  
Vol 9 (10) ◽  
Author(s):  
JohnE. Hachey ◽  
KiranK. Sharma ◽  
MauriceM. Moloney
Keyword(s):  
Shoot Regeneration ◽  
Brassica Campestris ◽  
Cotyledon Explants

scholarly journals Interaction between Explant Size and Cultivar Affects Shoot Organogenic Competence of Watermelon Cotyledons

HortScience ◽  
2000 ◽  
Vol 35 (4) ◽  
pp. 749-750 ◽  
Author(s):  
Michael E. Compton
Keyword(s):  
Shoot Regeneration ◽  
Significant Interaction ◽  
Adventitious Shoots ◽  
Adventitious Shoot ◽  
Shoot Formation ◽  
Proximal Region ◽  
Specific Region ◽  
Adventitious Shoot Regeneration ◽  
Cotyledon Explants

Organic competence of different explant sizes and locations on watermelon seedlings was determined by calculating the percentage of cotyledon explants that produced adventitious shoots. About 52% (214/412) of explants prepared from the proximal region of cotyledons formed shoots, whereas only ≈6% (24/411) of distal explants did so. Shoot formation was limited to the proximal end of basal explants but was not restricted to any specific region on distal ones. The percentage of explants that produced harvestable shoots was greater from basal halves than basal quarters in `Sweet Gem', `Crimson Sweet', and `Minilee', but explant size did not affect adventitious shoot regeneration of `Yellow Doll', resulting in significant interaction between cultivar and explant size. This study indicates that cultivars that respond poorly to in vitro procedures may have fewer cells competent for shoot regeneration, requiring special care during explant preparation.

scholarly journals In vitro Regeneration of Cabbage (Brassica oleracea L. var. Capitata) through Hypocotyl and Cotyledon Culture

1970 ◽  
Vol 17 (2) ◽  
pp. 131-136 ◽  
Author(s):  
M.K. Munshi ◽  
P.K. Roy ◽  
M.H. Kabir ◽  
G. Ahmed
Keyword(s):  
Shoot Regeneration ◽  
Callus Formation ◽  
In Vitro Regeneration ◽  
Multiple Shoots ◽  
Direct Regeneration ◽  
Cotyledon Explants ◽  
Cotyledon Culture ◽  
Best Response ◽  
Half Strength

The best response toward direct regeneration of multiple shoots from seven-dayold seedling was observed on half-strength MS supplemented with 0.5 mg/l BA. Hypocotyl and cotyledon explants produced highest percentage (73 and 66, respectively) of shoots. The maximum number of shoots (12) and the highest shoot length of 5.9 cm were also observed in this medium. On the other hand, indirect regeneration via callus was observed in cotyledonary explants. Maximum percentage of callus formation was observed on MS containing 1.0 mg/l 2,4-D and 0.5 mg/l NAA. Highest frequency of shoot regeneration was achieved on MS fortified with 2.0 mg/l BA and 0.1 mg/l NAA in cotyledon derived callus. Shoot regeneration was not obtained in hypocotyl-derived callus. Shoots rooted well when they were excised individually and implanted in halfstrength MS with 0.5 mg/l IBA in which 98% rooting was achieved within 10 - 12 days. The well rooted in vitro raised plantlets were successfully transferred to soil and their survival rate under natural environment was 86%. Key words: In vitro regeneration, Cabbage, Hypocotyl, Cotyledon  D.O.I. 10.3329/ptcb.v17i2.3233  Plant Tissue Cult. & Biotech. 17(2): 131-136, 2007 (December)

scholarly journals In vitro Plant Regeneration in Brassica spp.

2011 ◽  
Vol 21 (2) ◽  
pp. 127-134 ◽  
Author(s):  
Sanjida Rahman Mollika ◽  
R.H. Sarker ◽  
M.I. Hoque
Keyword(s):  
Plant Regeneration ◽  
Brassica Juncea ◽  
Shoot Regeneration ◽  
Brassica Campestris ◽  
Multiple Shoot ◽  
Root Induction ◽  
Best Response ◽  
Brassica Spp ◽  
Multiple Shoot Regeneration

MS with different concentrations and combinations of hormones were used for in vitro multiple shoot regeneration in two varieties of Brassica juncea, namely, BARI Sarisha-11 and BARI Sarisha-16 and one variety of Brassica campestris, Tori-7. The highest percentage of responsive explants towards the regeneration of shoots was obtained on MS with 2.0 mg/l BAP, 0.2 mg/l NAA and 0.5 mg/l Kn in case of BARI Sarisha-11 and BARI Sarisha-16. For Tori-7, 3.0 mg/l BAP and 0.2 mg/l NAA was best for obtaining maximum number of shoots per explant. Among three varieties, BARI Sarisha-11 showed best response in terms of shoot regeneration as well as number of shoot per explant. Days required for induction of shoots was also recorded to be lowest in BARI Sarisha-11. Best root induction in BARI Sarisha-11 and BARI Sarisha-16 was achieved on hormone free MS. After proper hardening the in vitro regenerated plantlets were successfully transplanted into soil. Interestingly some of the in vitro regenerated shoots produced in vitro flowers on regeneration media as well as hormone free MS. Key words: Plant regeneration, Brassica juncea, Brassica campestris D. O. I. 10.3329/ptcb.v21i2.10235 Plant Tissue Cult. & Biotech. 21(2): 127-134, 2011 (December)

scholarly journals Influence of Seedling Pretreatment and Explant Type on Watermelon Shoot Organogenesis

HortScience ◽  
1997 ◽  
Vol 32 (3) ◽  
pp. 514F-515 ◽  
Author(s):  
Michael E. Compton
Keyword(s):  
Plant Regeneration ◽  
Shoot Regeneration ◽  
Shoot Organogenesis ◽  
General Procedure ◽  
Shoot Formation ◽  
Light Environment ◽  
Explant Type ◽  
Regeneration Medium ◽  
Cotyledon Explants

Several methods have been published on shoot regeneration from watermelon cotyledon explants. The major differences in regeneration protocols include the light environment in which seeds are germinated and the cotyledon region used. The purpose of these experiments was to compare the two main protocols for plant regeneration and develop one general procedure. To fulfill this objective, seeds were germinated in vitro in darkness or 16-hr light photoperiod for 7 days. Cotyledon explants from four watermelon cultivars (`Crimson Sweet', `Minilee', `Sweet Gem', and `Yellow Doll') were prepared from both dark- and light-grown seedlings. Apical and basal halves were obtained by making a cut across the cotyledon width. Apical and basal quarters were made, for comparison, by cutting apical and basal halves longitudinally. All explants were incubated on shoot regeneration medium for 6 weeks followed by a 3-week cycle on shoot elonga-tion medium. The percentage of cotyledons with shoots was 1.7-fold greater for cotyledons derived from seedings incubated in darkness than those germinated in light. Shoot formation was about 10-fold greater for explants from cotyledon basal halves and quarters than apical halves and quarters. According to these results, the best watermelon regeneration protocol should consists of basal explants from in vitro-germinated seedlings incubated in the dark for 7 days.

scholarly journals In vitro callus and shoot regeneration in Enterolobium cyclocarpum (Jacq.) Grised. - a fast timber yielding species

2020 ◽  
Vol 12 (1) ◽  
pp. 74-89
Author(s):  
Michael S. AKINROPO ◽  
Benjamin E. AYISIRE ◽  
Ejeoghene R. OGBIMI
Keyword(s):  
Shoot Regeneration ◽  
Leaf Explants ◽  
Basal Medium ◽  
Multiple Shoots ◽  
Nodal Explants ◽  
Naphthalene Acetic Acid ◽  
Ms Medium ◽  
Cotyledon Explants ◽  
Enterolobium Cyclocarpum

This study was conducted to investigate the in vitro callus induction and rapid shoot regeneration potential in Enterolobium cyclocarpum, a plant native to central Mexico but widely introduced into Africa. The leaf, stem and nodal explants of E. cyclocarpum were cultured on full strength Murashige and Skoog (MS) medium supplemented with different concentrations of Cytokinins - Benzyladenine (BA) and/or Kinetin and Auxins - Naphthalene acetic acid (NAA) and/or 2,4-Dichlorophenoxylacetic acid (2,4-D) each alone and in combination.  The leaf explants did not respond to these treatments.  The Nodal explants were best for caulogenesis, while the explant responses were in the order- nodal > stem > cotyledon for callogenesis in MS medium supplemented with BA and/or Kin combined with NAA and/or 2,4-D. The varied combinations induced white compact callus.  The highest callus production was observed on MS medium supplemented with 2.7 µM NAA + 2.2 µM BA and 5.4 µM NAA alone.  Nodal and cotyledon explants developed callus and multiple shoots on MS supplemented with a combination of cytokinin (BA and/or Kin.) and auxin (NAA and/or 2,4-D). The maximum number of 3.98 ± 0.37 and 2.1±0.11 shoots/explants were recorded for nodal and cotyledon explants on MS medium supplemented with a combination of 8.8 µM BA+2.7 µM NAA and 2.2µM BA+2.7 µM NAA respectively.  On the basal medium, 10% of the excised shoots rooted successfully. Thus, this in vitro method can be exploited for conservation and mass propagation of this fast timber yielding tree and also utilized for embryogenesis studies.

Comparison of shoot regeneration potential from seedling explants of Australian cauliflower (Brassica oleracea var. botrytis) varieties

1998 ◽  
Vol 49 (8) ◽  
pp. 1261 ◽  
Author(s):  
Nicole A. Smith ◽  
Prem L. Bhalla
Keyword(s):  
Shoot Regeneration ◽  
Shoot Formation ◽  
Multiple Shoot ◽  
Root Explants ◽  
Hypocotyl Explants ◽  
Cotyledon Explants ◽  
Shoot Initiation ◽  
Seedling Explants ◽  
High Regeneration

Genetic engineering of crop plants relies on the development of efficient methods for the regeneration of viable shoots from cultured tissues. The objective of the present study was to develop a protocol for efficient shoot and plant regeneration from seedling explants of commercial cauliflower (B. oleracea var. botrytis) genotypes and to compare the regeneration capacity of the most commonly used explants: cotyledon, hypocotyl, and root. A combination of growth hormones including 6-benzylaminopurine, 1-naphthylacetic acid, and gibberellic acid was used in the MS-based medium, and factors influencing regeneration of shoots were investigated. Using the protocol described here, shoots from hypocotyl, cotyledon, and root explants of all the 11 genotypes tested were able to be regenerated. Root and hypocotyl explants produced more callus than cotyledon explants and also were more responsive to shoot regeneration, as a high percentage (>71% and >80% for hypocotyl and roots, respectively) of shoot initiation from these explants was observed. In addition, root and hypocotyl explants also produced more shoots per explant than cotyledon explants. The in vitro regenerated shoots were successfully rooted and acclimatised to glasshouse conditions. This study shows that seedling explants of cauliflower are amenable to multiple shoot formation with high regeneration frequencies, and could be used for genetic transformation experiments.

scholarly journals In vitro regeneration of three varieties of Brassica campestris L. grown in Bangladesh

2020 ◽  
Vol 55 (3) ◽  
pp. 181-188
Author(s):  
B Goswami ◽  
MI Hoque ◽  
S Khan ◽  
RH Sarker
Keyword(s):  
Shoot Regeneration ◽  
Brassica Campestris ◽  
In Vitro Regeneration ◽  
Shoot Formation ◽  
Multiple Shoot ◽  
Root Induction ◽  
Regeneration System ◽  
Cotyledonary Leaf ◽  
Best Response

A reproducible in vitro regeneration system was developed for three varieties of Brassica campestris L. namely Agrani, BINA Sarisha-10 and BINA Sarisha-6 using hypocotyl and cotyledonary leaf with petiole as explants. MS medium supplemented with 2.0 mg/l BAP and 0.2 mg/l IAA was found to be the best for the multiple shoot formation for all the three varieties. Among three varieties, BINA Sarisha-6 showed best response in terms of shoot regeneration as well as number of shoots per explant (9.0) using hypocotyls as explants. In case of Agrani and BINA Sarisha-10 highest number of shoot per explants were found 8.2 and 7.0, respectively. Interestingly in vitro regenerated shoots of BINA Sarisha-6 and BINA Sarisha-10 were produced in vitro flowers on shoot regeneration media. Best root induction in BINA Sarisha-6, Agrani and BINA Sarisha-10 was achieved on MS media supplemented with 0.5 mg/l IBA. After proper hardening, the in vitro regenerated plantlets were successfully transplanted into soil. Bangladesh J. Sci. Ind. Res.55(3), 181-188, 2020

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