scholarly journals In vitro regeneration of three varieties of Brassica campestris L. grown in Bangladesh

2020 ◽  
Vol 55 (3) ◽  
pp. 181-188
Author(s):  
B Goswami ◽  
MI Hoque ◽  
S Khan ◽  
RH Sarker
Keyword(s):  
Shoot Regeneration ◽  
Brassica Campestris ◽  
In Vitro Regeneration ◽  
Shoot Formation ◽  
Multiple Shoot ◽  
Root Induction ◽  
Regeneration System ◽  
Cotyledonary Leaf ◽  
Best Response

A reproducible in vitro regeneration system was developed for three varieties of Brassica campestris L. namely Agrani, BINA Sarisha-10 and BINA Sarisha-6 using hypocotyl and cotyledonary leaf with petiole as explants. MS medium supplemented with 2.0 mg/l BAP and 0.2 mg/l IAA was found to be the best for the multiple shoot formation for all the three varieties. Among three varieties, BINA Sarisha-6 showed best response in terms of shoot regeneration as well as number of shoots per explant (9.0) using hypocotyls as explants. In case of Agrani and BINA Sarisha-10 highest number of shoot per explants were found 8.2 and 7.0, respectively. Interestingly in vitro regenerated shoots of BINA Sarisha-6 and BINA Sarisha-10 were produced in vitro flowers on shoot regeneration media. Best root induction in BINA Sarisha-6, Agrani and BINA Sarisha-10 was achieved on MS media supplemented with 0.5 mg/l IBA. After proper hardening, the in vitro regenerated plantlets were successfully transplanted into soil. Bangladesh J. Sci. Ind. Res.55(3), 181-188, 2020

scholarly journals In vitro Plant Regeneration in Brassica spp.

2011 ◽  
Vol 21 (2) ◽  
pp. 127-134 ◽  
Author(s):  
Sanjida Rahman Mollika ◽  
R.H. Sarker ◽  
M.I. Hoque
Keyword(s):  
Plant Regeneration ◽  
Brassica Juncea ◽  
Shoot Regeneration ◽  
Brassica Campestris ◽  
Multiple Shoot ◽  
Root Induction ◽  
Best Response ◽  
Brassica Spp ◽  
Multiple Shoot Regeneration

MS with different concentrations and combinations of hormones were used for in vitro multiple shoot regeneration in two varieties of Brassica juncea, namely, BARI Sarisha-11 and BARI Sarisha-16 and one variety of Brassica campestris, Tori-7. The highest percentage of responsive explants towards the regeneration of shoots was obtained on MS with 2.0 mg/l BAP, 0.2 mg/l NAA and 0.5 mg/l Kn in case of BARI Sarisha-11 and BARI Sarisha-16. For Tori-7, 3.0 mg/l BAP and 0.2 mg/l NAA was best for obtaining maximum number of shoots per explant. Among three varieties, BARI Sarisha-11 showed best response in terms of shoot regeneration as well as number of shoot per explant. Days required for induction of shoots was also recorded to be lowest in BARI Sarisha-11. Best root induction in BARI Sarisha-11 and BARI Sarisha-16 was achieved on hormone free MS. After proper hardening the in vitro regenerated plantlets were successfully transplanted into soil. Interestingly some of the in vitro regenerated shoots produced in vitro flowers on regeneration media as well as hormone free MS. Key words: Plant regeneration, Brassica juncea, Brassica campestris D. O. I. 10.3329/ptcb.v21i2.10235 Plant Tissue Cult. & Biotech. 21(2): 127-134, 2011 (December)

scholarly journals In vitro regeneration protocol of Rauvolfia serpentina L.

2018 ◽  
Vol 53 (2) ◽  
pp. 133-138 ◽  
Author(s):  
S Khan ◽  
TA Banu ◽  
S Akter ◽  
B Goswami ◽  
M Islam ◽  
...  
Keyword(s):  
In Vitro Regeneration ◽  
Leaf Explants ◽  
Multiple Shoot ◽  
Nodal Segments ◽  
Root Induction ◽  
Ms Medium ◽  
Regeneration System ◽  
Rauvolfia Serpentina ◽  
Number Of Shoots

An efficient in vitro regeneration system was developed for Rauvolfia serpentina L. through direct and indirect organogenesis from nodal and leaf explants. Among the different growth regulators, MS medium supplemented with 2.0 mg/l BAP, 0.5mg/l IAA and 0.02mg/l NAA found best for the multiple shoot formation from nodal segments. In this combination 98% explants produced multiple shoots and the average number of shoots per explants is 13∙4. The frequency of callus induction and multiple shoot induction from leaves was highest 88% in MS medium supplemented with 2.0 mg/l BAP, where mean number of shoots/explants was 12.5. The highest frequency of root induction (80%) and mean number of roots/plantlets (10) were obtained on half strength of MS medium containing 0.2 mg/l IBA. The rooted plantlets were transferred for hardening following acclimatization and finally were successfully established in the field.Bangladesh J. Sci. Ind. Res.53(2), 133-138, 2018

scholarly journals In vitro Regeneration and Agrobacterium-mediated Genetic Transformation of Tomato (Lycopersicon esculentum Mill.)

1970 ◽  
Vol 19 (1) ◽  
pp. 101-111 ◽  
Author(s):  
Rakha Hari Sarker ◽  
Khaleda Islam ◽  
M.I. Hoque
Keyword(s):  
Lycopersicon Esculentum ◽  
Genetic Transformation ◽  
In Vitro Regeneration ◽  
Leaf Explants ◽  
Multiple Shoots ◽  
Multiple Shoot ◽  
Root Induction ◽  
Cotyledonary Leaf ◽  
Lycopersicon Esculentum Mill

Agrobacterium-mediated genetic transformation system has been developed for two tomato (Lycopersicon esculentum Mill.) varieties, namely Pusa Ruby (PR) and BARI Tomato-3 (BT-3). Prior to the establishment of transformation protocol cotyledonary leaf explants from the two varieties were cultured to obtain genotype independent in vitro regeneration. Healthy multiple shoot regeneration was obtained from the cut ends of cotyledonary leaf segments for both the varieties on MS containing 1.0 mg/l BAP and 0.1 mg/l IAA. The maximum root induction from the regenerated shoots was achieved on half the strength of MS medium supplemented with 0.2 mg/l IAA. The in vitro grown plantlets were successfully transplanted into soil where they flowered and produced fruits identical to those developed by control plants. Transformation ability of cotyledonary leaf explants was tested with Agrobacterium tumefaciens strain LBA4404 harboring binary plasmid pBI121, containing GUS and npt II genes. Transformed cotyledonary leaf explants were found to produce multiple shoots on MS containing 1.0 mg/l BAP and 0.1 mg/l IAA. Selection of the transformed shoots was carried out by gradually increasing the concentration of kanamycin to 200 mg/l since kanamycin resistant gene was used for transformation experiments. Shoots that survived under selection pressure were subjected to rooting. Transformed rooted plantlets were transferred to soil. Stable expression of GUS gene was detected in the various tissues from putatively transformed plantlets using GUS histochemical assay.  Key words: In vitro regeneration, transformation, tomato D.O.I. 10.3329/ptcb.v19i1.5004 Plant Tissue Cult. & Biotech. 19(1): 101-111, 2009 (June)

scholarly journals In vitro Regeneration in Three Varieties of White jute (Corchorus capsularis L.)

1970 ◽  
Vol 17 (1) ◽  
pp. 11-18 ◽  
Author(s):  
RH Sarker ◽  
GM Al-Amin ◽  
MI Hoque
Keyword(s):  
Shoot Regeneration ◽  
Multiple Shoots ◽  
Multiple Shoot ◽  
The Other ◽  
Nodal Explants ◽  
Root Induction ◽  
Corchorus Capsularis ◽  
Best Response ◽  
Multiple Shoot Regeneration

Healthy multiple shoot regeneration was observed from petiole-attached cotyledon (PC) explants of CVL-1 on MS containing 0.2 mg/l BAP and 1.0 mg/l IAA. On the other hand, the best response for multiple shoot regeneration in CVE-3 was obtained when the same explants were cultured on MS with 2.5 mg/l BAP and 0.5 mg/l NAA. However, the same explant of D-154 was found to show less responsive compared to other two varieties to produce multiple shoots. Cotyledonary nodal explants of all the three varieties were found to produce maximum number of multiple shoots on MS supplemented with 0.2 mg/l BAP and 1.0 mg/l IAA. Best root induction was observed at the base of the in vitro regenerated shoots on half the strength of MS supplemented with 0.3 mg/l IBA. The in vitro grown plantlets were successfully transplanted into soil. They grew up to maturity, flowered and fruited like the control plants.Key words: Regeneration, White jute, Corchorus capsularisDOI = 10.3329/ptcb.v17i1.1116Plant Tissue Cult. & Biotech. 17(1): 11-18, 2007 (June)

scholarly journals Establishment of in vitro regeneration protocol for Adhatoda vasica Nees

2016 ◽  
Vol 51 (1) ◽  
pp. 75-80 ◽  
Author(s):  
S Khan ◽  
S Akter ◽  
A Habib ◽  
TA Banu ◽  
M Islam ◽  
...  
Keyword(s):  
In Vitro Regeneration ◽  
Multiple Shoots ◽  
Shoot Formation ◽  
Multiple Shoot ◽  
Nodal Segments ◽  
Garden Soil ◽  
Root Induction ◽  
Ms Medium ◽  
Adhatoda Vasica

An in vitro regeneration protocol of Adhatoda vasica has been developed using excised nodal segments and juvenile leaves for multiple shoots regeneration directly or through callus induction. Explants were cultured on MS medium with different concentrations of IAA, NAA, BAP, GA3 and Kn singly or in combinations. MS medium supplemented with BAP (10.0 mg/l) was found best for multiple shoot formation, in which 93.33% explants produced multiple shoots. After two months, maximum number of multiple shoots were 10.6 ± 1.82, highest length of plantlets was 5.2 ± 2.20 cm. 100% calli formation were observed on MS medium supplemented with IAA (0.05 mg/l) + NAA (0.05 mg/l) + BAP (1.0 mg/l). Callus initiation started after 14 days and gave light green colored callus. Best callus mediated shoot regeneration was found on MS+10.0 mg/l BAP medium. Root induction of in vitro raised shoots was best on ½ MS + IBA (1.0 mg/l). Well rooted plantlets were transferred to plastic pots containing garden soil and compost in a ratio of 2:1 for hardening. The ultimate survival rate under natural condition was about 80%.Bangladesh J. Sci. Ind. Res. 51(1), 75-80, 2016

scholarly journals In vitro Plant Regeneration of Four Local Varieties of Chickpea (Cicer arietinum L.) Grown in Bangladesh

1970 ◽  
Vol 46 (3) ◽  
pp. 379-384 ◽  
Author(s):  
TA Banu ◽  
RH Sarker ◽  
MI Hoque
Keyword(s):  
In Vitro Regeneration ◽  
Multiple Shoots ◽  
Mature Embryo ◽  
Multiple Shoot ◽  
Direct Organogenesis ◽  
Ms Medium ◽  
Regeneration System ◽  
Best Response ◽  
Multiple Shoot Regeneration

In vitro regeneration system was developed through direct organogenesis from decapitated mature embryo explants of locally grown four chickpea varieties, namely, Barichhola-4, Hyprochhola, Binachhola-3 and Binachhola-4. Best response towards multiple shoot regeneration was obtained on MS medium supplemented with 0.5 mg/l BAP, 0.5 mg/l Kn, 0.2 mg/l NAA along with double concentrations of CaCl2 and NH4NO3. However good shoot health and expanded leaf was found on MS medium containing 1.0 mg/l kn. Apart from this, few experiments were conducted with decapitated embryo attached cotyledon. Using this explants highest number of multiple shoots were obtained on MSB medium containing 4× micronutrients of MS medium with 3.0 mg/l BAP and 0.04 mg/l NAA in all four varieties. Shoots regenerated on 1.0 mg/l kn supplemented medium showed good response towards rooting on MS medium supplemented with 0.2 mg/l IBA in all four varieties. It was observed that micrografting is an alternative technique to in vitro rooting in chickpea. Key words: In vitro regeneration; Decapitated embryo; Chickpea. DOI: http://dx.doi.org/10.3329/bjsir.v46i3.9047 BJSIR 2011; 46(3): 379-384

scholarly journals In- vitro regeneration of an important medicinal plant Centella asiatica L. Urban

2012 ◽  
Vol 47 (3) ◽  
pp. 269-272 ◽  
Author(s):  
Dr Jaheduzzaman ◽  
MA Habib ◽  
S Akter ◽  
NA Banu ◽  
RB Rahman ◽  
...  
Keyword(s):  
Shoot Regeneration ◽  
In Vitro Regeneration ◽  
Centella Asiatica ◽  
Multiple Shoot ◽  
Garden Soil ◽  
Nodal Segment ◽  
Root Induction ◽  
Ms Medium ◽  
Multiple Shoot Regeneration

An in vitro multiple shoot regeneration protocol has been developed for Centella asiatica L. Urban from the nodal and shoot tips explants using MS medium with different concentrations and combinations of growth regulators. Multiple shoot regeneration was observed from both the explants on MS containing either BAP alone or in combination with IAA, NAA and Kn. Of the two explants, nodal segment showed comparatively better response towards multiple shoot regeneration. Maximum multiple shoots were found on MS supplemented with 1.0 mg/L BAP and 0.4 mg/L NAA. For root induction, well-developed shoots were excised and cultured on both MS and half strength of MS with various concentrations of IBA, IAA and NAA. Profuse healthy rooting was obtained on MS medium containing 0.2 mg/L IBA. The well rooted plantlets were successfully transplanted to the garden soil and their survival rate under natural condition was 90-95 %. DOI: http://dx.doi.org/10.3329/bjsir.v47i3.13058 Bangladesh J. Sci. Ind. Res. 47(3), 269-272, 2012

scholarly journals An Efficient Protocol for High-frequency Direct Multiple Shoot Regeneration from Internodes of Peppermint (Mentha x piperita)

2010 ◽  
Vol 5 (12) ◽  
pp. 1934578X1000501
Author(s):  
Sanjog T. Thul ◽  
Arun K. Kukreja
Keyword(s):  
Shoot Regeneration ◽  
Multiple Shoots ◽  
Shoot Elongation ◽  
Shoot Formation ◽  
Multiple Shoot ◽  
Ms Medium ◽  
Mentha X Piperita ◽  
Half Strength ◽  
Multiple Shoot Regeneration

A simple, repeatable and efficient protocol for direct multiple shoot regeneration from internodal explants has been defined in peppermint ( Mentha x piperita var. Indus). In vitro regenerated shoots of peppermint were excised into 4 to 8 mm long internodes and cultured on Murashige and Skoog's medium supplemented with different cytokinins. In the hormonal assay, 3.0 mg L-l zeatin or 6-isopentenyl adenine independently supplemented to half strength MS medium exhibited multiple shoot regeneration, while thiaduzorn (0.1-3.0 mg L−1) showed no morphogenetic effect. A maximum of 85% in vitro cultured explants showed multiple shoot formation with an average of 7 shoots per explant on MS medium supplemented with zeatin. Multiple shoots were initiated within three weeks of cultivation. Internodes with regenerated multiple shoots were transferred to half - strength MS medium without supplementing with any plant growth hormone for shoot elongation and rhizogenesis. Rooted plants acclimatized and grew to maturity under glasshouse conditions. The plantlets developed were phenotypically identical to the parent plant and exhibited 96 % survival.

scholarly journals In vitro Shoot Cultures of Tupistra albiflora K. Larsen

2018 ◽  
Vol 17 (5) ◽  
pp. 405-411
Author(s):  
Jiraporn PALEE
Keyword(s):  
Agar Medium ◽  
Multiple Shoots ◽  
Shoot Formation ◽  
Multiple Shoot ◽  
Naphthalene Acetic Acid ◽  
Root Induction ◽  
Shoot Cultures ◽  
Ms Medium ◽  
In Vitro Shoots

To evaluate an efficient protocol for the micropropagation of Tupistra albiflora K. Larsen, the effects of N6-benzylaminopurine (BA) and naphthalene acetic acid (NAA) concentrations on multiple shoot and root induction were examined. In vitro shoots were used as the explant materials which were cultured on Murashige and Skoog (MS) agar medium supplemented with 0, 1, 2, 3 and 4 mg/L BA for 4 weeks to induce multiple shoots. It was found that the MS medium containing 3 mg/L BA induced 100 % shoot formation with the highest number of 3.2 shoots per explant (2.4-fold significantly higher than the control). For root induction, in vitro shoots were cultured on MS agar medium supplemented with 0, 1, 2, 3 and 4 mg/L NAA for 8 weeks. The results showed that the MS medium containing 1 mg/L NAA induced 100 % root formation with the highest number of 6.6 roots per explant (1.8-fold significantly higher than the control).

In vitro mass propagation of Piper betle L

2019 ◽  
Vol 48 (3) ◽  
pp. 559-566
Author(s):  
Salim Khan ◽  
Barna Goswami ◽  
Shahina Akter ◽  
Mousona Islam ◽  
Afsana Huq Noon ◽  
...  
Keyword(s):  
Nodal Explants ◽  
Direct Regeneration ◽  
Leaf Segment ◽  
Piper Betle ◽  
Nodal Segment ◽  
Root Induction ◽  
Regeneration System ◽  
Petiole Explants ◽  
Best Response

An efficient in vitro regeneration system was developed for Piper betle L. through direct and indirect organogenesis from nodal segment, leaf segment and petiole explants. Highest direct regeneration was recorded when nodal explants were cultured on MS with 1.0 mg/l BAP and 1.0 mg/l Kn where 80% explants produced multiple shoots and the average number of shoots per explants were 3.20. Remarkable results on callus induction and shoot initiation were observed when the explants cultured on MS + 2.0 mg/l BAP + 0.5 mg/l Kn + 1.0 mg/l IAA. It was observed that nodal explants were showed best response on shoot/explants 13.2 ± 4.5 after 8 weeks of callus culture on MS medium with 0.5 mg/l BAP. The best response towards root induction was observed on half strength of MS with 0.25 mg/l IBA. The well rooted plants were successfully acclimatized and transferred to soil.

Sign in / Sign up

Export Citation Format

Share Document