Effects of stimulation and rest on the ultrastructure of the excitatory neuromuscular junctions of Locusta migratoria L.

1979 ◽  
Vol 203 (3) ◽  
Author(s):  
R.P. Botham ◽  
D.J. Beadle ◽  
R.J. Hart ◽  
C. Potter ◽  
R.G. Wilson
Keyword(s):  
Neuromuscular Junctions ◽  
Locusta Migratoria

Glutamate uptake after stimulation-induced depletion of vesicle numbers in neuromuscular junctions of Locusta migratoria L.

1979 ◽  
Vol 203 (3) ◽  
Author(s):  
R.P. Botham ◽  
D.J. Beadle ◽  
R.J. Hart ◽  
C. Potter ◽  
R.G. Wilson
Keyword(s):  
Neuromuscular Junctions ◽  
Glutamate Uptake ◽  
Locusta Migratoria

Zinc iodide-osmium tetroxide reaction: its effect on the synaptic vesicles in locust neuromuscular junctions and its chemical basis

1978 ◽  
Vol 36 (2) ◽  
pp. 614-615
Author(s):  
M. Reinecke ◽  
Ch. Walther
Keyword(s):  
Synaptic Vesicles ◽  
Osmium Tetroxide ◽  
Neuromuscular Junctions ◽  
Motor Nerve ◽  
Locusta Migratoria ◽  
Nerve Terminals ◽  
Neurobiological Research ◽  
Zinc Iodide ◽  
Electron Lucent

The zinc iodide-osmium tetroxide reaction (ZIO) was first used in neurobiological research by Maillet (Bull. Ass. Anat. 53, 233; 1968). Subsequently several authors have shown that, under appropriate conditions, ZIO stains mainly the interior of synaptic vesicles. The substrate of this reaction is under discussion, since ZIO can also react with other subcellular structures in a variety of tissues, e. g. mitochondria, endoplasmic reticulum, dictyosomes and lysosomes. Additionally, in vitro substances as different as some aminoacids, catecholamines, aldehydes and phospholipids (Pellegrino de Iraldi, Experientia 33, 1; 1977) can yield black precipitations with ZIO.Our studies were done with the motor nerve terminals at the femoral retractor unguis muscle of the locust (Locusta migratoria). These terminals are chiefly the endings of excitatory motoraxons and are characterized by the presence of electron lucent vesicles and by an accumulation of mitochondria.

Innervation of the ventral diaphragm of the locust (Locusta migratoria)

1977 ◽  
Vol 69 (1) ◽  
pp. 23-32
Author(s):  
M. Peters
Keyword(s):  
Electrical Stimulation ◽  
Electrical Properties ◽  
Electrical Activity ◽  
Neuromuscular Junctions ◽  
Motor Nerve ◽  
Locusta Migratoria ◽  
Posterior Segment ◽  
Muscle Fibres ◽  
Inspiratory Muscles ◽  
Motor Neurones

1. Innervation and some electrical properties of the locust ventral diaphragm were investigated with electrophysiological and histological methods. 2. Muscle fibres are coupled electrically. Electrical stimulation evokes a graded active membrane response. 3. Each segment is innervated by four motor neurones as follows. Two motor neurones are situated in each abdominal ganglion. Branches of their axons supply the ventral diaphragm in the respective and the next posterior segment. 4. This pattern of innervation was confirmed by axonal Co and Ni staining of the motor nerve endings. 5. Neuromuscular junctions are excitatory. EPSPs show summation but no facilitation. 6. Spontaneous electrical activity of the diaphragm is to a certain degree coupled to activity of the main inspiratory muscles.

The Action of Iontophoretically Applied Glutamate on Insect Muscle Fibres

1968 ◽  
Vol 49 (1) ◽  
pp. 83-93
Author(s):  
R. BERÁNEK ◽  
P. L. MILLER
Keyword(s):  
Equilibrium Point ◽  
Neuromuscular Junctions ◽  
Schistocerca Gregaria ◽  
Locusta Migratoria ◽  
Extracellular Recording ◽  
Fibre Surface ◽  
Adductor Muscle ◽  
Muscle Fibres ◽  
Glass Capillary ◽  
Insect Muscle

1. Electrophoretic application of L-glutamate from glass capillary micro-pipettes was used to investigate the ‘spot sensitivity’ of the membrane of coxal adductor muscle fibres from adult specimens of Schistocerca gregaria Forskål and Locusta migratoria (L.). 2. Circumscribed spots could be detected on the fibre surface where brief applications of L-glutamate produced transient depolarizations (glutamate potentials). 3. Extracellular recording of excitatory junction potentials revealed that focal points of glutamate sensitivity are closely related to, and probably identical with, neuromuscular junctions. 4. Large doses readily de-sensitized the membrane to L-glutamate for periods greatly exceeding the duration of the glutamate potentials. 5. In chronically denervated muscles peaks of sensitivity could still be detected. 6. Spots sensitive to L-glutamate were not depolarized by D-glutamate. 7. The equilibrium point for glutamate potentials coincides with the equilibrium point of miniature excitatory potentials and lies between -10 and -25 mV.

The innervation of toad lymph hearts: An ultrastructural study

1992 ◽  
Vol 50 (1) ◽  
pp. 898-899
Author(s):  
A.M. Pucci ◽  
C. Fruschelli ◽  
A. Rebuffat ◽  
M. Guarna ◽  
C. Alessandrini ◽  
...  
Keyword(s):  
Ultrastructural Study ◽  
Neuromuscular Junctions ◽  
Nerve Fibers ◽  
Lack Of Information ◽  
Lymph Heart ◽  
Muscular Pump ◽  
Structure And Ultrastructure ◽  
Heart Wall

Amphibians have paired muscular pump organs, called “lymph heart”, which rhythmically pump back the lymph from the large subcutaneous lymph sacs into the veins. The structure and ultrastructure of these organs is well known but to date there is a lack of information about the innervation of lymph hearts. Therefore has been carried out an ultrastructural study in order to study the distribution of the nerve fibers, and the morphology of the neuromuscular junctions in the lymph heart wall.

Co-Culture of ES Cell-Derived Motor Neurons and Myotubes Show the Formation of Neuromuscular Junctions and Changes in Gene Expression

2006 ◽  
Vol 22 (06) ◽  
Author(s):  
Aleid Ruijs ◽  
Tateki Kubo ◽  
Jae Song ◽  
Milan Ranka ◽  
Mark Randolph ◽  
...  
Keyword(s):  
Gene Expression ◽  
Motor Neurons ◽  
Neuromuscular Junctions ◽  
Es Cell
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