Influence of primary callus induction conditions on the establishment of barley cell suspensions yielding regenerable protoplasts

1994 ◽  
Vol 13 (10) ◽  
Author(s):  
Hideyuki Funatsuki ◽  
Makoto Kihara
Keyword(s):  
Callus Induction ◽  
Cell Suspensions ◽  
Primary Callus ◽  
Barley Cell

scholarly journals In vitro somatic embryogenesis of superior clones of robusta coffee from Lampung, Indonesia: Effect of genotypes and callus induction media

2020 ◽  
Vol 21 (8) ◽  
Author(s):  
Dwi Hapsoro ◽  
Rahmadyah Hamiranti ◽  
Yusnita Yusnita
Keyword(s):  
Somatic Embryogenesis ◽  
Callus Induction ◽  
Leaf Explants ◽  
Regeneration Medium ◽  
Embryogenic Calli ◽  
Robusta Coffee ◽  
Primary Callus ◽  
Ascorbic Acids ◽  
Superior Clones

Abstract. Hapsoro D, Hamiranti R, Yusnita Y. 2020. In vitro somatic embryogenesis of superior clones of robusta coffee from Lampung, Indonesia: Effect of genotypes and callus induction media. Biodiversitas 21: 3811-3817. This study aimed to investigate the effects of genotypes and primary callus induction media on somatic embryogenesis of superior robusta coffee clones of Lampung. Leaf explants of clones Tugusari, Komari, Tugino, and Wanto were cultured on two types of primary callus induction media (PCIM). PCIM1 consisted of half-strength MS salts, 30 gL-1 sucrose, added with (mgL-1) 0.1 thiamine-HCl, 0.5 nicotinic acids, 0.5 pyridoxine-HCl, 100 Myo-inositol, 200 ascorbic acids, 150 citric acids, and 1 benzyl adenine. PCIM2 consisted of NPCM salts, 30 gL-1 sucrose, added with (mgL-1) 15 thiamine-HCl, 1 nicotinic acid, 1 pyridoxine-HCl, 2 glycines, 130 Myo-inositol, 200 ascorbic acids, 150 citric acids, 1 2,4-dichlorophenoxyacetic acid, and 2 thidiazuron. The highest percentage (100%) of primary callus formation was found in Komari and Wanto clones. PCIM2 resulted in more primary calli than PCIM1. When subcultured to embryogenic callus induction medium, primary calli of clone Komari and Wanto developed into a high percentage of embryogenic calli, while those of the other two turned brown and died. PCIM2-derived primary calli developed into more embryogenic calli. When subcultured on somatic embryo (SE) regeneration medium, these calli underwent the formation of SE of various stages. When subcultured to plant regeneration medium, these SEs developed into plantlets.

CALLUS INDUCTION AND A PRELIMINARY ATTEMPT TO OBTAIN CELL SUSPENSIONS FROM EMBRYOS AND LEAVES OF JUGLANS REGIA L.

1987 ◽  
pp. 683-686
Author(s):  
R. Rodríguez ◽  
M.A. Revilla ◽  
C. Pérez ◽  
C. Cabranes ◽  
R.S. Tamés
Keyword(s):  
Callus Induction ◽  
Juglans Regia ◽  
Cell Suspensions ◽  
Preliminary Attempt

scholarly journals Pengaruh Komposisi Media Dasar dan Jenis Eksplan terhadap Pembentukan Embrio Somatik Kakao

2016 ◽  
Vol 3 (3) ◽  
pp. 127
Author(s):  
Nur Ajijah
Keyword(s):  
Somatic Embryo ◽  
Callus Induction ◽  
Callus Formation ◽  
Basal Medium ◽  
Induction Medium ◽  
Explant Type ◽  
Salt Medium ◽  
Basal Salt Medium ◽  
Primary Callus ◽  
Callus Induction Medium

<em>The composition of basal medium determines the regeneration success of in vitro culture. The study aimed to evaluate the effect of basal medium in the primary callus induction medium and explant type on the formation of cacao somatic embryo. The research was conducted at the Tissue Culture Laboratory of IAARD, Bogor, from June 2014 to December 2015. Primary callus induction derived from staminoid and petal explants of ICCRI 4 clone used two types of basal medium, i.e. DKW+ 9 µM 2.4-D + 1.16 µM kinetin or WPM + 9 µM 2.4-D + 1.16 µM kinetin.</em> <em>After 14 days, callus was subcultured onto secondary callus induction medium (WPM + 2.4-D 9 </em><em>μM + kinetin 0.58 μM), and then onto DKW medium without growth regulators to induce the formation of somatic embryo. The research was designed in two-factor factorial design with five replications. The first factor was the type of basal medium on the primary callus induction medium (DKW and WPM) and the second factor was the type of explants (petal and staminoid). The results showed significant interaction effect between basal medium type and explant type on the formation of callus and somatic embryo of cacao. The highest percentage of callus formation was derived from staminoid explants on the DKW basal salt medium (92.5%). However, the highest percentage of somatic embryo formation and the number of somatic embryo per explant were obtained from DKW basal salt medium with petal explants (36.5% and 2.3).</em> <em>Therefore, t</em><em>he use of DKW basal salt medium and petal explant were recommended for the induction of somatic embryo of the ICCRI 4 clone.</em>

A pre-embedding, protein a-gold immunolabelling technique for cytocentrifuged cell monolayers for lm and tem

1986 ◽  
Vol 44 ◽  
pp. 220-221
Author(s):  
K. Chien ◽  
I.P. Shintaku ◽  
A.F. Sassoon ◽  
R.L. Van de Velde ◽  
R. Heusser
Keyword(s):  
Cell Surface ◽  
Staining Method ◽  
Protein A ◽  
Surface Antigens ◽  
Cell Suspensions ◽  
Cellular Morphology ◽  
Cellular Phenotype ◽  
Cell Surface Antigens ◽  
Cell Monolayers ◽  
Diagnostic Histopathology

Identification of cellular phenotype by cell surface antigens in conjunction with ultrastructural analysis of cellular morphology can be a useful tool in the study of biologic processes as well as in diagnostic histopathology. In this abstract, we describe a simple pre-embedding, protein A-gold staining method which is designed for cell suspensions combining the handling convenience of slide-mounted cell monolayers and the ability to evaluate specimen staining specificity prior to EM embedding.

Pravastatin: A tool for investigating the availability of mevalonate metabolites for primary and secondary metabolism in Catharanthus roseus cell suspensions

1996 ◽  
Vol 98 (4) ◽  
pp. 803-809 ◽  
Author(s):  
Nadine Imbault ◽  
Martine Thiersault ◽  
Paulette Duperon ◽  
Abdellah Benabdelmouna ◽  
Pierre Doireau
Keyword(s):  
Secondary Metabolism ◽  
Catharanthus Roseus ◽  
Cell Suspensions ◽  
Primary And Secondary Metabolism

Adenine salvage activity during callus induction and plant growth

1994 ◽  
Vol 90 (4) ◽  
pp. 739-747 ◽  
Author(s):  
Diana Lee ◽  
Barbara A. Moffatt
Keyword(s):  
Plant Growth ◽  
Callus Induction

Uptake of 13 C-glucose by cell suspensions of carrot (Daucus carota) measured by in vivo NMR: Cycling of triose-, pentose- and hexose-phosphates

2000 ◽  
Vol 108 (2) ◽  
pp. 125-133
Author(s):  
Janhendrik Krook ◽  
Dick Vreugdenhil ◽  
Cor Dijkema ◽  
Linus H.W. van der Plas
Keyword(s):  
Daucus Carota ◽  
Cell Suspensions ◽  
In Vivo Nmr ◽  
Hexose Phosphates

A semi-automatic method for the production of primary cell suspensions

1972 ◽  
Vol 22 (3) ◽  
pp. 335-341
Author(s):  
F. C. Belton ◽  
D. J. Garriock
Keyword(s):  
Cell Suspensions ◽  
Primary Cell ◽  
Automatic Method

Optimum Conditions for the Preparation of Red Blood Cell Suspensions for ABO Antibody Titration

2015 ◽  
Vol 5 (1) ◽  
pp. 15 ◽  
Author(s):  
Seon Joo Kang ◽  
Sung Ran Cho ◽  
Young Ae Lim
Keyword(s):  
Blood Cell ◽  
Red Blood Cell ◽  
Cell Suspensions ◽  
Optimum Conditions

DEVELOPMENT OF ISOLOGOUS TRANSPLANTS OF CELL SUSPENSIONS OF THE FETAL PANCREAS IN THE RAT

1974 ◽  
Vol 77 (1_Suppl) ◽  
pp. S97 ◽  
Author(s):  
K. H. Usadel ◽  
U. Schwedes ◽  
U. Leuschner ◽  
K. Schöffling
Keyword(s):  
Cell Suspensions ◽  
Fetal Pancreas
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