Molecular analysis of the capsid protein gene of a german isolate of barley mild mosaic virus

1993 ◽  
Vol 12 (4) ◽  
Author(s):  
U. Schlichter ◽  
A. Sohn ◽  
E. Peerenboom ◽  
J. Schell ◽  
H.-H. Steinbi�
Keyword(s):  
Mosaic Virus ◽  
Capsid Protein ◽  
Molecular Analysis ◽  
Protein Gene ◽  
Mild Mosaic ◽  
Capsid Protein Gene ◽  
Mild Mosaic Virus ◽  
German Isolate

scholarly journals Molecular analysis of the capsid protein gene of a german isolate of barley mild mosaic virus

1993 ◽  
Vol 12 (5) ◽  
pp. 298-298 ◽  
Author(s):  
U. Schlichter ◽  
A. Sohn ◽  
E. Peerenboom ◽  
J. Schell ◽  
H. -H. Steinbiβ
Keyword(s):  
Mosaic Virus ◽  
Capsid Protein ◽  
Molecular Analysis ◽  
Protein Gene ◽  
Mild Mosaic ◽  
Capsid Protein Gene ◽  
Mild Mosaic Virus ◽  
German Isolate

scholarly journals Properties and Capsid Protein Gene Sequence of a Korean Isolate of Barley Mild Mosaic Virus.

1996 ◽  
Vol 62 (4) ◽  
pp. 397-401 ◽  
Author(s):  
Kui-Jae LEE ◽  
Satoshi KASHIWAZAKI ◽  
Tadaaki HIBI ◽  
In-Young SO
Keyword(s):  
Mosaic Virus ◽  
Capsid Protein ◽  
Gene Sequence ◽  
Protein Gene ◽  
Korean Isolate ◽  
Mild Mosaic ◽  
Capsid Protein Gene ◽  
Mild Mosaic Virus

scholarly journals cDNA cloning and nucleotide sequence of the wheat streak mosaic virus capsid protein gene

1991 ◽  
Vol 72 (3) ◽  
pp. 499-504 ◽  
Author(s):  
C. L. Niblett ◽  
K. R. Zagula ◽  
L. A. Calvert ◽  
T. L. Kendall ◽  
D. M. Stark ◽  
...  
Keyword(s):  
Nucleotide Sequence ◽  
Mosaic Virus ◽  
Capsid Protein ◽  
Cdna Cloning ◽  
Protein Gene ◽  
Wheat Streak Mosaic Virus ◽  
Capsid Protein Gene ◽  
Virus Capsid ◽  
Virus Capsid Protein

The Occurrence of Barley Mild Mosaic Virus (BaMMV) in China and the Nucleotide Sequence of its Coat Protein Gene

1999 ◽  
Vol 147 (4) ◽  
pp. 229-234 ◽  
Author(s):  
T. Zheng ◽  
Y. Cheng ◽  
J. P. Chen ◽  
J. F. Antoniw ◽  
M. J. Adams
Keyword(s):  
Nucleotide Sequence ◽  
Coat Protein ◽  
Mosaic Virus ◽  
Coat Protein Gene ◽  
Protein Gene ◽  
Mild Mosaic ◽  
Mild Mosaic Virus

scholarly journals Assessment of Yam mild mosaic virus coat protein gene sequence diversity reveals the prevalence of cosmopolitan and African group of isolates in Ghana and Nigeria

2020 ◽  
Vol 23 ◽  
pp. 100156
Author(s):  
Chukwuemeka K. Nkere ◽  
Emmanuel Otoo ◽  
Gabriel I. Atiri ◽  
Joseph Onyeka ◽  
Gonçalo Silva ◽  
...  
Keyword(s):  
Coat Protein ◽  
Mosaic Virus ◽  
Coat Protein Gene ◽  
Gene Sequence ◽  
Protein Gene ◽  
Mild Mosaic ◽  
Coat Protein Gene Sequence ◽  
Mild Mosaic Virus ◽  
African Group ◽  
Virus Coat

Cloning and sequence analysis of the coat protein gene of barley mild mosaic virus

1993 ◽  
Vol 27 (1) ◽  
pp. 79-89 ◽  
Author(s):  
I.J. Foulds ◽  
V.J. Lea ◽  
C. Sidebottom ◽  
C.M. James ◽  
R.E. Boulton ◽  
...  
Keyword(s):  
Sequence Analysis ◽  
Coat Protein ◽  
Mosaic Virus ◽  
Coat Protein Gene ◽  
Protein Gene ◽  
Mild Mosaic ◽  
Mild Mosaic Virus

scholarly journals First report of costus stripe mosaic virus infecting Tradescantia spathacea plants in Brazil

Plant Disease ◽  
2021 ◽  
Author(s):  
Gabriel Madoglio Favara ◽  
Felipe Franco de Oliverira ◽  
Camila Geovana Ferro ◽  
Heron Delgado Kraide ◽  
Eike Yudi Nishimura Carmo ◽  
...  
Keyword(s):  
Mosaic Virus ◽  
Protein Gene ◽  
Infected Plant ◽  
Nucleotide Sequences ◽  
Mild Mosaic ◽  
Rt Pcr ◽  
Chenopodium Amaranticolor ◽  
Capsid Protein Gene ◽  
Total Rna ◽  
First Report

Tradescantia spathacea (family Commelinaceae) is cultivated worldwide as an ornamental (Golczyk et al., 2013) and as medicinal plant (Tan et al., 2020). In 2019, 90 of ~180 plants of T. spathacea, grown in two beds of 4 m2 and exhibiting leaf mosaic were found in an experimental area at ESALQ/USP (Piracicaba municipality, São Paulo state, Brazil). Potyvirus-like flexuous filamentous particles were observed by transmission electron microscopy in foliar extracts of two symptomatic plants stained with 1% uranyl acetate. Total RNA was extracted using the Purelink viral RNA/DNA kit (Thermo Fisher Scientific) from leaves of two symptomatic plants and separately subjected to a reverse transcription polymerase chain reaction (RT-PCR). The potyviruses degenerate pairs of primers CIFor/CIRev (Ha et al. 2008), which amplifies a fragment corresponding to part of the cylindrical inclusion protein gene, and WCIEN/PV1 (Maciel et al. 2011), which amplifies a fragment containing part of the capsid protein gene and the 3′ untranslated region, were used. The expected amplicons (~700bp) were obtained from both total RNA extracts. Two amplicons from one sample were purified using the Wizard SV Gel and PCR Clean-Up System kit (Promega) and directly sequenced in both directions at Macrogen Inc (Seoul, South Korea). The obtained nucleotide sequences (GenBank MW430005 and MW503934) shared 95.32% and 97.79% nucleotide identity, respectively, with the corresponding sequences of the Brazilian isolate of the potyvirus costus stripe mosaic virus (CoSMV, MK286375) (Alexandre et al. 2020). Extract from an infected plant of T. spathacea was mechanically inoculated in 10 healthy plants of T. spathacea and two plants each of the following species: Capsicum annuum, Chenopodium amaranticolor, Commelina benghalensis, Datura stramonium, Gomphrena globosa, Nicandra physaloides, Nicotiana tabacum cvs. Turkish and Samsun, Solanum lycopersicum, T. palida, and T. zebrina. All T. spathacea plants exhibited mosaic and severe leaf malformation. C. benghalensis plants developed mild mosaic, whereas infected T. zebrina plants were asymptomatic. The plants of other species were not infected. RT-PCR with specific CoSMV primers CoSMVHC-F and CoSMVHC-R (Alexandre et al. 2020) confirmed the infection. Nucleotide sequences of amplicons obtained from experimentally inoculated T. spathacea and T. zebrina (MW430007 and MW430008) shared 94.56% and 94.94% identity with the corresponding sequence of a Brazilian CoSMV isolate (MK286375). None of eight virus-free plants of T. spathacea inoculated with CoSMV using Aphis craccivora exhibited symptoms, nor was CoSMV detected by RT-PCR. Lack of CoSMV transmission by A. solanella, Myzus persicae, and Uroleucon sonchi was previously reported (Alexandre et al. 2020). T. spathacea plants are commonly propagated vegetatively, and by seeds. Virus-free seeds, if available, can provide an efficient and easy way to obtain healthy plants. Only three viruses were reported in plants of the genus Tradescantia: Commelina mosaic virus, tradescantia mild mosaic virus, and a not fully characterized potyvirus (Baker and Zettler, 1988; Ciuffo et al., 2006; Kitajima 2020). CoSMV was recently reported infecting Costus spiralis and C. comosus (Alexandre et al. 2020). As far as we know, this is the first report of CoSMV infecting T. spathacea plants.

Nucleotide sequence of coat protein gene of yam mild mosaic virus, isolated in Papua New Guinea

1999 ◽  
Vol 144 (7) ◽  
pp. 1415-1419 ◽  
Author(s):  
S. Fuji ◽  
I. Mitobe ◽  
H. Nakamae ◽  
K. T. Natsuaki
Keyword(s):  
Nucleotide Sequence ◽  
Coat Protein ◽  
Mosaic Virus ◽  
Papua New Guinea ◽  
Coat Protein Gene ◽  
Protein Gene ◽  
New Guinea ◽  
Mild Mosaic ◽  
Mild Mosaic Virus
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