Nucleotide sequence of coat protein gene of yam mild mosaic virus, isolated in Papua New Guinea

1999 ◽  
Vol 144 (7) ◽  
pp. 1415-1419 ◽  
Author(s):  
S. Fuji ◽  
I. Mitobe ◽  
H. Nakamae ◽  
K. T. Natsuaki
Keyword(s):  
Nucleotide Sequence ◽  
Coat Protein ◽  
Mosaic Virus ◽  
Papua New Guinea ◽  
Coat Protein Gene ◽  
Protein Gene ◽  
New Guinea ◽  
Mild Mosaic ◽  
Mild Mosaic Virus

The Occurrence of Barley Mild Mosaic Virus (BaMMV) in China and the Nucleotide Sequence of its Coat Protein Gene

1999 ◽  
Vol 147 (4) ◽  
pp. 229-234 ◽  
Author(s):  
T. Zheng ◽  
Y. Cheng ◽  
J. P. Chen ◽  
J. F. Antoniw ◽  
M. J. Adams
Keyword(s):  
Nucleotide Sequence ◽  
Coat Protein ◽  
Mosaic Virus ◽  
Coat Protein Gene ◽  
Protein Gene ◽  
Mild Mosaic ◽  
Mild Mosaic Virus

scholarly journals Assessment of Yam mild mosaic virus coat protein gene sequence diversity reveals the prevalence of cosmopolitan and African group of isolates in Ghana and Nigeria

2020 ◽  
Vol 23 ◽  
pp. 100156
Author(s):  
Chukwuemeka K. Nkere ◽  
Emmanuel Otoo ◽  
Gabriel I. Atiri ◽  
Joseph Onyeka ◽  
Gonçalo Silva ◽  
...  
Keyword(s):  
Coat Protein ◽  
Mosaic Virus ◽  
Coat Protein Gene ◽  
Gene Sequence ◽  
Protein Gene ◽  
Mild Mosaic ◽  
Coat Protein Gene Sequence ◽  
Mild Mosaic Virus ◽  
African Group ◽  
Virus Coat

Cloning and sequence analysis of the coat protein gene of barley mild mosaic virus

1993 ◽  
Vol 27 (1) ◽  
pp. 79-89 ◽  
Author(s):  
I.J. Foulds ◽  
V.J. Lea ◽  
C. Sidebottom ◽  
C.M. James ◽  
R.E. Boulton ◽  
...  
Keyword(s):  
Sequence Analysis ◽  
Coat Protein ◽  
Mosaic Virus ◽  
Coat Protein Gene ◽  
Protein Gene ◽  
Mild Mosaic ◽  
Mild Mosaic Virus

scholarly journals First Report and Molecular Characterization of Yam mild mosaic virus in Dioscorea alata on the Island of Martinique

Plant Disease ◽  
2000 ◽  
Vol 84 (2) ◽  
pp. 200-200 ◽  
Author(s):  
M. Bousalem ◽  
S. Dallot
Keyword(s):  
Monoclonal Antibodies ◽  
Mosaic Virus ◽  
Papua New Guinea ◽  
New Guinea ◽  
Enzyme Linked Immunosorbent Assay ◽  
Dioscorea Alata ◽  
Mild Mosaic ◽  
First Report ◽  
Mild Mosaic Virus ◽  
The Caribbean

Naturally infected Dioscorea alata plants showing mild mosaic were collected in 1998 on the island of Martinique in the Caribbean. Isolates were first screened by double-antibody sandwich enzyme-linked immunosorbent assay (ELISA) with monoclonal antibodies raised against Yam mosaic virus (YMV) and antigen-coated plate ELISA with universal potyvirus monoclonal antibodies (Agdia, Elkhart, IN). A positive reaction was obtained only with the universal potyvirus antiserum. Immunocapture reverse-transcriptase polymerase chain reaction was performed for specific detection of Yam mild mosaic virus (YMMV [3]) and YMV. A product with the predicted size of 249 bp was obtained with YMMV primers. YMMV is a recently recognized distinct potyvirus infecting D. alata in West Africa and the South Pacific (2–4). It was originally described as Yam virus I and is synonymous with Dioscorea alata virus (4). To characterize the YMMV Martinique isolate, total RNA was extracted, and universal potyvirus degenerate primers (1) were used to amplify a 700-bp fragment that included the core and C-terminal region of the coat protein (CP) and 3′ untranslated region (3′UTR). Sequence information generated (EMBL AJ250336) from the cloned fragment was compared with sequences of other yam potyviruses. Sequence comparisons of the partial CP (453 nt) showed a similarity of 94.6% (amino acids [aa]) with the YMMV isolate from Papua New Guinea (EMBL AB022424 [2]); 72.2% (aa) with the Japanese yam mosaic virus (JYMV) isolate (EMBL AB016500); and 67 to 73% (aa) with 27 YMV isolates. These sequences are most diverse in the 3′UTR, which showed a similarity of 72.8% with the YMMV Papua New Guinea isolate, 30% with the JYMV isolate, and 26% with the YMV isolates. These results confirm, as previously shown by S. Fuji et al. (2), that YMMV should be classified as a new potyvirus of yam. This is the first report of the natural occurrence of YMMV in the Caribbean. References: (1) Colinet et al. Phytopathology 84:65, 1994. (2) S. Fuji et al. Arch Virol. 144:1415, 1999. (3) R. A. Munford and S. E. Seal. J. Virol. Methods 69:73, 1997. (4) B. O. Odu et al. Ann. Appl. Biol. 134:65, 1999.

scholarly journals Nucleotide sequence of the tamarillo mosaic virus coat protein gene

1990 ◽  
Vol 18 (23) ◽  
pp. 7166-7166 ◽  
Author(s):  
Robin M. Eagles ◽  
Richard C. Gardner ◽  
Richard L.S. Forster
Keyword(s):  
Nucleotide Sequence ◽  
Coat Protein ◽  
Mosaic Virus ◽  
Coat Protein Gene ◽  
Protein Gene ◽  
Virus Coat Protein ◽  
Virus Coat

scholarly journals Molecular cloning and nucleotide sequence of coat protein gene of turnip mosaic virus

1990 ◽  
Vol 18 (18) ◽  
pp. 5555-5555 ◽  
Author(s):  
Ling-Jie Kong ◽  
Rong-Xiang Fang ◽  
Zheng-Hua Chen ◽  
Ke-Qiang Mang
Keyword(s):  
Nucleotide Sequence ◽  
Coat Protein ◽  
Mosaic Virus ◽  
Molecular Cloning ◽  
Coat Protein Gene ◽  
Protein Gene ◽  
Turnip Mosaic Virus

scholarly journals Biological, Pathological, and Molecular Characteristics of a New Potyvirus, Dendrobium Chlorotic Mosaic Virus, Infecting Dendrobium Orchid

Plant Disease ◽  
2019 ◽  
Vol 103 (7) ◽  
pp. 1605-1612 ◽  
Author(s):  
Chih-Hung Huang ◽  
Chia-Hsing Tai ◽  
Ruey-Song Lin ◽  
Chung-Jan Chang ◽  
Fuh-Jyh Jan
Keyword(s):  
Amino Acid ◽  
Nucleotide Sequence ◽  
Coat Protein ◽  
Amino Acid Sequence ◽  
Mosaic Virus ◽  
Coat Protein Gene ◽  
Nucleotide Sequence Identity ◽  
Amino Acid Sequence Identity ◽  
Protein Gene ◽  
Sequence Identity

Dendrobium smillieae is one of the popular orchids in Taiwan. This report describes a new potyvirus tentatively named Dendrobium chlorotic mosaic virus (DeCMV) causing chlorotic and mosaic symptoms in D. smillieae. Enzyme-linked immunosorbent assay (ELISA) tests using six antisera against orchid-infecting viruses revealed that only a monoclonal antibody against the potyvirus group reacted positively with crude saps prepared from a symptomatic dendrobium orchid. Potyvirus-like, flexuous, filamentous particles were observed under an electron microscope, measuring approximately 700 to 800 nm in length and 11 to 12 nm in diameter. Sequence analyses revealed that DeCMV coat protein gene shared 59.6 to 66.0% nucleotide sequence identity and 57.6 to 66.0% amino acid sequence identity, whereas the DeCMV complete genome shared 54.1 to 57.3% nucleotide sequence identity and 43.7 to 49.5% amino acid sequence identity with those other known potyviruses. These similarity levels were much lower than the criteria set for species demarcation in potyviruses. Thus, DeCMV can be considered a new potyvirus. The whole DeCMV genome contains 10,041 nucleotides (GenBank accession no. MK241979) and encodes a polyprotein that is predicted to produce 10 proteins by proteolytic cleavage. In a pathogenicity test, results of inoculation assays demonstrated that DeCMV can be transmitted to dendrobium orchids by grafting and mechanical inoculation, as verified by ELISA and western blot analyses using the DeCMV polyclonal antiserum and by reverse transcription polymerase chain reaction using the coat protein gene-specific primers. The inoculated orchids developed similar chlorotic and mosaic symptoms. In conclusion, DeCMV is a novel orchid-infecting potyvirus, and this is the first report of a new potyvirus that infects dendrobium orchids in Taiwan.

scholarly journals The nucleotide sequence of apple mosaic virus coat protein gene has no similarity with other Bromoviridae coat protein genes

1994 ◽  
Vol 75 (10) ◽  
pp. 2847-2850 ◽  
Author(s):  
R. H. Alrefai ◽  
P. J. Shiel ◽  
L. L. Domier ◽  
C. J. D'Arcy ◽  
P. H. Berger ◽  
...  
Keyword(s):  
Nucleotide Sequence ◽  
Coat Protein ◽  
Mosaic Virus ◽  
Coat Protein Gene ◽  
Protein Gene ◽  
Virus Coat Protein ◽  
Virus Coat
Sign in / Sign up

Export Citation Format

Share Document