Lysine overproducer mutants with an altered dihydrodipicolinate synthase from protoplast culture of Nicotiana sylvestris (Spegazzini and Comes)

1984 ◽  
Vol 68-68 (1-2) ◽  
pp. 11-20 ◽  
Author(s):  
I. Negrutiu ◽  
A. Cattoir-Reynearts ◽  
I. Verbruggen ◽  
M. Jacobs
Keyword(s):  
Protoplast Culture ◽  
Nicotiana Sylvestris ◽  
Dihydrodipicolinate Synthase

Structure of dihydrodipicolinate synthase of Nicotiana sylvestris reveals novel quaternary structure

1997 ◽  
Vol 274 (4) ◽  
pp. 608-621 ◽  
Author(s):  
Stefan Blickling ◽  
Hans-Georg Beisel ◽  
Damir Bozic ◽  
Jörg Knäblein ◽  
Bernd Laber ◽  
...  
Keyword(s):  
Quaternary Structure ◽  
Nicotiana Sylvestris ◽  
Dihydrodipicolinate Synthase

Isolation, Culture and Plant Regeneration From Protoplasts of Nicotiana debneyi

1980 ◽  
Vol 7 (6) ◽  
pp. 635 ◽  
Author(s):  
WR Scowcroft ◽  
PJ Larkin
Keyword(s):  
Acetic Acid ◽  
Plant Regeneration ◽  
Indole Acetic Acid ◽  
High Efficiency ◽  
Protoplast Culture ◽  
Callus Cultures ◽  
Dna Uptake ◽  
Mesophyll Protoplasts ◽  
Nicotiana Debneyi ◽  
Protoplast Division

Mesophyll protoplasts of two genetically distinct genotypes of N. debneyi were cultured with sustained division following a plating efficiency in excess of 50%. Fully fertile mature plants were regenerated from callus cultures derived from protoplasts. Shoots were induced in medium containing 1 mg/l 6-benzylaminopurine and 0.5 mg/I indole acetic acid. The repeatably high efficiency of protoplast culture was used to evaluate the quantitative effects of two drugs, kanamycin and trimethoprim, which effectively inhibited colony formation at concentrations of 100 and 50 �g/ml, respectively. An enhancer of DNA uptake, poly-L-ornithine, had virtually no effect on sustained protoplast division at a concentration of 7.5 �g/ml or less.

scholarly journals Carbon Partitioning and Growth of a Starchless Mutant of Nicotiana sylvestris

1992 ◽  
Vol 99 (4) ◽  
pp. 1449-1454 ◽  
Author(s):  
Steven C. Huber ◽  
Kenneth R. Hanson
Keyword(s):  
Nicotiana Sylvestris ◽  
Carbon Partitioning ◽  
Starchless Mutant
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