Quantitative succinate dehydrogenase analysis in normal and ragged-red muscle fibers

1991 ◽  
Vol 96 (3) ◽  
pp. 251-253 ◽  
Author(s):  
H. Reichmann ◽  
D. Wildenauer
Keyword(s):  
Succinate Dehydrogenase ◽  
Muscle Fibers ◽  
Red Muscle

Motoneuron and muscle fiber succinate dehydrogenase activity in control and overloaded plantaris

1991 ◽  
Vol 71 (4) ◽  
pp. 1589-1592 ◽  
Author(s):  
G. R. Chalmers ◽  
R. R. Roy ◽  
V. R. Edgerton
Keyword(s):  
Succinate Dehydrogenase ◽  
Muscle Fiber ◽  
Muscle Fibers ◽  
Cell Types ◽  
Mitochondrial Proteins ◽  
Succinate Dehydrogenase Activity ◽  
Fiber Size ◽  
The Right ◽  
Gastrocnemius Muscles ◽  
Selective Increase

To determine the level of coordination in succinate dehydrogenase (SDH) activity between plantaris motoneurons and muscle fibers, the soleus and gastrocnemius muscles were bilaterally excised in four cats to subject the plantaris to functional overload (FO). Five normal cats served as controls. Twelve weeks after surgery the right plantaris in each cat was injected with horseradish peroxidase to identify plantaris motoneurons. SDH activity then was measured in a population of plantaris motoneurons and muscle fibers in each cat. Control motoneurons and muscle fibers had similar mean SDH activities and a similar relationship between cell size and SDH activity. After FO, muscle fiber size doubled and mean muscle fiber SDH activity halved. Motoneuron mean SDH activity and size were unaffected by FO. Total SDH activity was unchanged in both the motoneurons and muscle fibers after FO. These changes suggest a selective increase in contractile proteins with little or no modulation of mitochondrial proteins in the muscle fibers, because total SDH activity was unchanged in muscle fibers after FO. These data demonstrate that although mean SDH activities were similar in control motoneurons and muscle fibers, mean SDH activities in these two cell types can change independently.

A fluid-driven soft robotic fish inspired by fish muscle architecture

2022 ◽  
Author(s):  
Sijia Liu ◽  
Yingjie Wang ◽  
Zhennan Li ◽  
Miao Jin ◽  
Lei Ren ◽  
...  
Keyword(s):  
Connective Tissue ◽  
Structural Design ◽  
Muscle Fibers ◽  
The Body ◽  
Optimum Frequency ◽  
Muscle System ◽  
Key Innovation ◽  
Biological Studies ◽  
Passive Deformation ◽  
Red Muscle

Abstract Artificial fish-like robots developed to date often focus on the external morphology of fish and have rarely addressed the contribution of the structure and morphology of biological muscle. However, biological studies have proven that fish utilize the contraction of muscle fibers to drive the protective flexible connective tissue to swim. This paper introduces a pneumatic silicone structure prototype inspired by the red muscle system of fish and applies it to the fish-like robot named Flexi-Tuna. The key innovation is to make the fluid-driven units simulate the red muscle fiber bundles of fish and embed them into a flexible tuna-like matrix. The driving units act as muscle fibers to generate active contraction force, and the flexible matrix as connective tissue to generate passive deformation. Applying alternant pressure to the driving units can produce a bending moment, causing the tail to swing. As a result, the structural design of Flexi-Tuna has excellent bearing capacity compared with the traditional cavity-type and keeps the body smooth. On this basis, a general method is proposed for modeling the fish-like robot based on the independent analysis of the active and passive body, providing a foundation for Flexi-Tuna’s size design. Followed by the robot’s static and underwater dynamic tests, we used finite element static analysis and fluid numerical simulation to compare the results. The experimental results showed that the maximum swing angle of the tuna-like robot reached 20°, and the maximum thrust reached 0.185 N at the optimum frequency of 3.5 Hz. In this study, we designed a unique system that matches the functional level of biological muscles. As a result, we realized the application of fluid-driven artificial muscle to bionic fish and expanded new ideas for the structural design of flexible bionic fish.

Intracellular distribution of succinate dehydrogenase activity in skeletal muscle fibers of geese

1984 ◽  
Vol 62 (2) ◽  
pp. 235-240 ◽  
Author(s):  
H. J. Swatland
Keyword(s):  
Skeletal Muscle ◽  
Succinate Dehydrogenase ◽  
Dehydrogenase Activity ◽  
Adenosine Triphosphatase ◽  
Muscle Fibers ◽  
Fiber Types ◽  
Succinate Dehydrogenase Activity ◽  
Individual Muscle ◽  
Concentric Zone ◽  
Pectoralis Muscles

Samples of iliotibialis anterior and pectoralis muscles were taken from five ganders (Anser domesticus). Serial transverse sections were reacted for succinate dehydrogenase (SDH) and alkali-stable adenosine triphosphatase (ATPase). The distribution of SDH activity within individual muscle fibers was measured with a scanning photometer. In many individual fibers, SDH activity was stronger in the periphery than in the axis. This gradient was steepest (−0.034 ± 0.019 absorbance units per concentric zone of 2 μm diameter measurements) in pectoralis fibers with strong SDH activity. In the pectoralis, radial gradients were correlated with fiber area so that the smallest fibers tended to have the steepest gradients of SDH activity. However, this relationship was reversed in fibers with strong ATPase and weak SDH activity in the iliotibialis anterior, and the largest fibers tended to have the steepest gradients. In all fiber types of both muscles, fibers with greater mean SDH activity tended to have steeper gradients.

scholarly journals GLYCOGEN SYNTHETASE AND PHOSPHORYLASE IN RED AND WHITE MUSCLE OF RAT AND RHESUS MONKEY

1966 ◽  
Vol 14 (7) ◽  
pp. 549-559 ◽  
Author(s):  
ROSE MARY BOCEK ◽  
CLARISSA H. BEATTY
Keyword(s):  
Rhesus Monkey ◽  
White Muscle ◽  
Muscle Fibers ◽  
Supernatant Fraction ◽  
Glycogen Synthetase ◽  
Red Muscle ◽  
Histochemical Evidence ◽  
Red And White Muscles

Homogenates of red and white muscles from rats and monkeys were assayed for total phosphorylase and phosphorylase a and for the total and independent forms of glycogen synthetase. Total and phosphorylase a activities were higher in the supernatant fraction of homogenates of white as compared with red muscle from both rats and monkeys. Both forms of phosphorylase were higher in white muscle from rats when assayed on whole homogenates. The total and d form of glycogen synthetase activities were higher in red muscle from both species of animals. The ratio of I/total synthetase was 2- to 3-fold higher in muscle from monkeys as compared with that from rats. These results support histochemical evidence that phosphorylase is higher in white muscle fibers and glycogen synthetase is higher in red muscle fibers.

Free autografting of entire limb muscles in the cat: histochemistry and biochemistry

1978 ◽  
Vol 44 (3) ◽  
pp. 431-437 ◽  
Author(s):  
L. C. Maxwell ◽  
J. A. Faulkner ◽  
S. A. Mufti ◽  
A. M. Turowski
Keyword(s):  
Succinate Dehydrogenase ◽  
Dehydrogenase Activity ◽  
Muscle Fibers ◽  
Cross Sectional Area ◽  
Sectional Area ◽  
Succinate Dehydrogenase Activity ◽  
Cross Sectional ◽  
The Mean ◽  
Fast Twitch

Fifty extensor digitorium longus muscles of 25 cats were autografted, 33 with and 17 without prior denervation. After 50 days, no significant differences were observed between predenervated and nonpredenervated autografts. Autografted muscles weighed 48% of the weight of control muscles. Few original muscle fibers survived and within 2 wk autografts contained regenerating muscle fibers. The mean cross-sectional area of muscle fibers in the autografts reached 125% of the value for control nontransplanted muscles. The mean percentage of fibers classified high oxidative in autografted muscles was 67% of values for control muscles. SDH activity of autografted muscle homogenates reached 55% of control values. Up to 60 days after surgery autografts had only fast-twitch fibers. At 170 days autografts remained 95% fast twitch in composition. Revascularization began within 4 days, but the capillary to fiber ratio of long term autografts reached only 60% of control values. Although fiber hypertrophy suggests that cats use autografted muscles, lower than control succinate dehydrogenase activity may result from altered recruitment.

Cytoplasm-to-myonucleus ratios and succinate dehydrogenase activities in adult rat slow and fast muscle fibers

1994 ◽  
Vol 275 (1) ◽  
pp. 39-49 ◽  
Author(s):  
Brian S. Tseng ◽  
Christine E. Kasper ◽  
V. Reggie Edgerton
Keyword(s):  
Succinate Dehydrogenase ◽  
Muscle Fibers ◽  
Fast Muscle ◽  
Adult Rat

Comparison of biochemical and physiological characteristics among white, pink, and red muscle fibers in carp (cultured)

2000 ◽  
Vol 66 (3) ◽  
pp. 586-593 ◽  
Author(s):  
Abdul Jabarsyah ◽  
Mutsuyosi Tsuchimoto ◽  
Osamu Yada ◽  
Yasutoshi Kozuru ◽  
Toshio Miyake ◽  
...  
Keyword(s):  
Muscle Fibers ◽  
Physiological Characteristics ◽  
Red Muscle
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