BIOCHEMICAL VERIFICATION OF QUANTITATIVE HISTOCHEMICAL ANALYSIS OF SUCCINATE DEHYDROGENASE ACTIVITY IN SKELETAL MUSCLE FIBERS

1992 ◽  
Vol 24 (Supplement) ◽  
pp. S126
Author(s):  
D. Criswell ◽  
S. Powers ◽  
F. Lieu ◽  
Harold Silverman ◽  
S. Dodd
Keyword(s):  
Skeletal Muscle ◽  
Succinate Dehydrogenase ◽  
Dehydrogenase Activity ◽  
Muscle Fibers ◽  
Histochemical Analysis ◽  
Succinate Dehydrogenase Activity ◽  
Skeletal Muscle Fibers

Intracellular distribution of succinate dehydrogenase activity in skeletal muscle fibers of geese

1984 ◽  
Vol 62 (2) ◽  
pp. 235-240 ◽  
Author(s):  
H. J. Swatland
Keyword(s):  
Skeletal Muscle ◽  
Succinate Dehydrogenase ◽  
Dehydrogenase Activity ◽  
Adenosine Triphosphatase ◽  
Muscle Fibers ◽  
Fiber Types ◽  
Succinate Dehydrogenase Activity ◽  
Individual Muscle ◽  
Concentric Zone ◽  
Pectoralis Muscles

Samples of iliotibialis anterior and pectoralis muscles were taken from five ganders (Anser domesticus). Serial transverse sections were reacted for succinate dehydrogenase (SDH) and alkali-stable adenosine triphosphatase (ATPase). The distribution of SDH activity within individual muscle fibers was measured with a scanning photometer. In many individual fibers, SDH activity was stronger in the periphery than in the axis. This gradient was steepest (−0.034 ± 0.019 absorbance units per concentric zone of 2 μm diameter measurements) in pectoralis fibers with strong SDH activity. In the pectoralis, radial gradients were correlated with fiber area so that the smallest fibers tended to have the steepest gradients of SDH activity. However, this relationship was reversed in fibers with strong ATPase and weak SDH activity in the iliotibialis anterior, and the largest fibers tended to have the steepest gradients. In all fiber types of both muscles, fibers with greater mean SDH activity tended to have steeper gradients.

scholarly journals Assay of succinate dehydrogenase activity by the tetrazolium method: evaluation of an improved technique in skeletal muscle fractions.

1980 ◽  
Vol 28 (5) ◽  
pp. 408-412 ◽  
Author(s):  
J D Green ◽  
H T Narahara
Keyword(s):  
Skeletal Muscle ◽  
Ethyl Acetate ◽  
Succinate Dehydrogenase ◽  
Dehydrogenase Activity ◽  
Inhibitory Effects ◽  
Succinate Dehydrogenase Activity ◽  
Low Concentrations ◽  
Mitochondrial Fractions ◽  
Mitochondrial Suspension ◽  
Improved Technique

An improved spectrophotometric method for measuring succinate dehydrogenase (EC 1.3.99.1) activity with the use of 2-(p-iodophenyl)-3-(p-nitrophenyl)-5-phenyltetrazolium chloride (INT) is described. The procedure has been evaluated in mitochondrial fractions and homogenates of frog skeletal muscle. For mitochondrial suspensions, extraction of formazan with alcohol was found to be superior to extraction with ethyl acetate. For homogenates, complete extraction of formazan required sequential treatment with alcohol and ethyl acetate; the generally employed procedure of extracting once with ethyl acetate alone led to serious underestimation of the amount of formazan in the tissue. Observations of mitochondrial suspension incubated with various concentrations of INT led to the selection of 0.8 mM INT for optimal results. Higher concentrations, although commonly used, can exert undesirable inhibitory effects on succinate dehydrogenase activity, especially at low concentrations of mitochondria and after longer periods of incubation. The problem of instability of succinate dehydrogenase was solved by the addition of buffer at pH 7.5.

Free autografting of entire limb muscles in the cat: histochemistry and biochemistry

1978 ◽  
Vol 44 (3) ◽  
pp. 431-437 ◽  
Author(s):  
L. C. Maxwell ◽  
J. A. Faulkner ◽  
S. A. Mufti ◽  
A. M. Turowski
Keyword(s):  
Succinate Dehydrogenase ◽  
Dehydrogenase Activity ◽  
Muscle Fibers ◽  
Cross Sectional Area ◽  
Sectional Area ◽  
Succinate Dehydrogenase Activity ◽  
Cross Sectional ◽  
The Mean ◽  
Fast Twitch

Fifty extensor digitorium longus muscles of 25 cats were autografted, 33 with and 17 without prior denervation. After 50 days, no significant differences were observed between predenervated and nonpredenervated autografts. Autografted muscles weighed 48% of the weight of control muscles. Few original muscle fibers survived and within 2 wk autografts contained regenerating muscle fibers. The mean cross-sectional area of muscle fibers in the autografts reached 125% of the value for control nontransplanted muscles. The mean percentage of fibers classified high oxidative in autografted muscles was 67% of values for control muscles. SDH activity of autografted muscle homogenates reached 55% of control values. Up to 60 days after surgery autografts had only fast-twitch fibers. At 170 days autografts remained 95% fast twitch in composition. Revascularization began within 4 days, but the capillary to fiber ratio of long term autografts reached only 60% of control values. Although fiber hypertrophy suggests that cats use autografted muscles, lower than control succinate dehydrogenase activity may result from altered recruitment.

Sign in / Sign up

Export Citation Format

Share Document