rna12 +, a gene of Saccharomyces cerevisiae involved in pre-rRNA maturation. Characterization of a temperature-sensitive mutant, cloning and sequencing of the gene

1992 ◽  
Vol 232 (2) ◽  
pp. 304-312 ◽  
Author(s):  
Shuang Liang ◽  
Lefa Alksne ◽  
Jonathan R. Warner ◽  
François Lacroute
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Temperature Sensitive ◽  
Cloning And Sequencing ◽  
Sensitive Mutant ◽  
Temperature Sensitive Mutant ◽  
Rrna Maturation

Putative GTPase Gtr1p genetically interacts with the RanGTPase cycle in Saccharomyces cerevisiae

1996 ◽  
Vol 109 (9) ◽  
pp. 2311-2318 ◽  
Author(s):  
N. Nakashima ◽  
N. Hayashi ◽  
E. Noguchi ◽  
T. Nishimoto
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Negative Regulator ◽  
Temperature Sensitive ◽  
Nucleotide Exchange ◽  
Sensitive Mutant ◽  
Temperature Sensitive Mutant ◽  
Importin Alpha ◽  
Nucleotide Exchange Factor ◽  
Cold Sensitive ◽  
Gtpase Cycle

In order to identify a protein interacting with RCC1, a guanine nucleotide-exchange factor for the nuclear GTPase Ran, we isolated a series of cold-sensitive suppressors of mtr1-2, a temperature-sensitive mutant of the Saccharomyces cerevisiae RCC1 homologue. One of the isolated suppressor mutants was mutated in the putative GTPase Gtr1p, being designated as gtr1-11. It also suppressed other alleles of mtr1-2, srm1-1 and prp20-1 in contrast to overexpression of the S. cerevisiae Ran/TC4 homologue Gsp1p, previously reported to suppress prp20-1, but not mtr1-2 or srm1-1. Furthermore, gtr1-11 suppressed the rna1-1, temperature-sensitive mutant of the Gsp1p GTPase-activating protein, but not the srp1-31, temperature-sensitive mutant of the S. cerevisiae importin alpha homologue. mtr1-2, srm1-1 and prp20-1 were also suppressed by overexpression of the mutated Gtr1p, Gtr1-11p. In summary, Gtr1p that was localized in the cytoplasm by immunofluoresence staining was suggested to function as a negative regulator for the Ran/TC4 GTPase cycle.

Isolation and characterization of further defective clones of a temperature sensitive mutant (ts-1) of respiratory syncytial virus

1977 ◽  
Vol 54 (1-2) ◽  
pp. 53-60 ◽  
Author(s):  
Linda S. Richardson ◽  
T. J. Schnitzer ◽  
R. B. Belshe ◽  
Ena Camargo ◽  
D. A. Prevar ◽  
...  
Keyword(s):  
Respiratory Syncytial Virus ◽  
Temperature Sensitive ◽  
Sensitive Mutant ◽  
Temperature Sensitive Mutant ◽  
Isolation And Characterization ◽  
Syncytial Virus
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