Assignment of adenosine deaminase complexing protein (ADCP) gene(s) to human chromosome 2 in rodent-human somatic cell hybrids

1981 ◽  
Vol 59 (4) ◽  
pp. 317-323 ◽  
Author(s):  
Elly Herbschleb-Voogt ◽  
Karl-Heinz Grzeschik ◽  
Peter L. Pearson ◽  
P. Meera Khan
Keyword(s):  
Human Chromosome ◽  
Somatic Cell ◽  
Adenosine Deaminase ◽  
Chromosome 2 ◽  
Somatic Cell Hybrids ◽  
Human Somatic Cell ◽  
Cell Hybrids

Analysis of tumor suppressor gene on human chromosome 9 in mouse x human somatic cell hybrids

1994 ◽  
Vol 20 (5) ◽  
pp. 391-400 ◽  
Author(s):  
Bruce W. Porterfield ◽  
Olufunmilayo I. Olopade ◽  
Janet D. Rowley ◽  
Manuel O. Diaz
Keyword(s):  
Tumor Suppressor ◽  
Human Chromosome ◽  
Somatic Cell ◽  
Tumor Suppressor Gene ◽  
Suppressor Gene ◽  
Chromosome 9 ◽  
Somatic Cell Hybrids ◽  
Human Somatic Cell ◽  
Cell Hybrids

Assignment1 of β-centractin (CTRN2) to human chromosome 2 bands q11.1→q11.2 with somatic cell hybrids and in situ hybridization

1999 ◽  
Vol 84 (1-2) ◽  
pp. 48-49 ◽  
Author(s):  
S.H. Elsea ◽  
I.B. Clark ◽  
R.C. Juyal ◽  
D.J. Meyer ◽  
D.I. Meyer ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Human Chromosome ◽  
Somatic Cell ◽  
Chromosome 2 ◽  
Somatic Cell Hybrids ◽  
Cell Hybrids

Aconitase (E.C. 4.2.1.3) mitochondrial locus mapped to human chromosome 22: Studies with Chinese hamster-human somatic cell hybrids

1978 ◽  
Vol 16 (7-8) ◽  
pp. 751-756 ◽  
Author(s):  
Robert S. Sparkes ◽  
T. Mohandas ◽  
Maryellen C. Sparkes ◽  
Jeffery D. Shulkin
Keyword(s):  
Human Chromosome ◽  
Somatic Cell ◽  
Chinese Hamster ◽  
Chromosome 22 ◽  
Somatic Cell Hybrids ◽  
Human Somatic Cell ◽  
Cell Hybrids ◽  
Mitochondrial Locus

Segregation of recessive phenotypes in somatic cell hybrids: role of mitotic recombination, gene inactivation, and chromosome nondisjunction

1981 ◽  
Vol 1 (4) ◽  
pp. 336-346
Author(s):  
C E Campbell ◽  
R G Worton
Keyword(s):  
Somatic Cell ◽  
Chinese Hamster ◽  
Mitotic Recombination ◽  
Chinese Hamster Cell ◽  
Gene Inactivation ◽  
Chromosome Loss ◽  
Resistance Locus ◽  
Chromosome 2 ◽  
Somatic Cell Hybrids ◽  
Cell Hybrids

Somatic cell hybrids heterozygous at the emetine resistance locus (emtr/emt+) or the chromate resistance locus (chrr/chr+) are known to segregate the recessive drug resistance phenotype at high frequency. We have examined mechanisms of segregation in Chinese hamster cell hybrids heterozygous at these two loci, both of which map to the long arm of Chinese hamster chromosome 2. To follow the fate of chromosomal arms through the segregation process, our hybrids were also heterozygous at the mtx (methotrexate resistance) locus on the short arm of chromosome 2 and carried cytogenetically marked chromosomes with either a short-arm deletion (2p-) or a long-arm addition (2q+). Karyotype and phenotype analysis of emetine- or chromate-resistant segregants from such hybrids allowed us to distinguish four potential segregation mechanisms: (i) loss of the emt+- or chr+-bearing chromosome; (ii) mitotic recombination between the centromere and the emt or chr loci, giving rise to homozygous resistant segregants; (iii) inactivation of the emt+ or chr+ alleles; and (iv) loss of the emt+- or chr+-bearing chromosome with duplication of the homologous chromosome carrying the emtr or chrr allele. Of 48 independent segregants examined, only 9 (20%) arose by simple chromosome loss. Two segregants (4%) were consistent with a gene inactivation mechanism, but because of their rarity, other mechanisms such as mutation or submicroscopic deletion could not be excluded. Twenty-one segregants (44%) arose by either mitotic recombination or chromosome loss and duplication; the two mechanisms were not distinguishable in that experiment. Finally, in hybrids allowing these two mechanisms to be distinguished, 15 segregants (31%) arose by chromosome loss and duplication, and none arose by mitotic recombination.

Human chromosome 16 physical map: Mapping of somatic cell hybrids using multiplex PCR deletion analysis of sequence tagged sites

Genomics ◽  
1991 ◽  
Vol 10 (4) ◽  
pp. 1047-1052 ◽  
Author(s):  
Robert I. Richards ◽  
Katherine Holman ◽  
Sharon Lane ◽  
Grant R. Sutherland ◽  
David F. Callen
Keyword(s):  
Human Chromosome ◽  
Somatic Cell ◽  
Multiplex Pcr ◽  
Physical Map ◽  
Deletion Analysis ◽  
Somatic Cell Hybrids ◽  
Chromosome 16 ◽  
Cell Hybrids ◽  
Sequence Tagged Sites
Sign in / Sign up

Export Citation Format

Share Document