Neuronal types in the spinal dorsal gray of the turtle Chrysemys d'orbigny: a Golgi study

1993 ◽  
Vol 272 (2) ◽  
pp. 355-368 ◽  
Author(s):  
A. Fern�ndez ◽  
M. Radmilovich ◽  
O. Trujillo-Cen�z
Keyword(s):  
Golgi Study ◽  
Spinal Dorsal ◽  
Neuronal Types

A Golgi study of neuronal types in the neostriatum of monkeys

1976 ◽  
Vol 114 (2) ◽  
pp. 245-256 ◽  
Author(s):  
Marian DiFiglia ◽  
Pedro Pasik ◽  
Tauba Pasik
Keyword(s):  
Golgi Study ◽  
Neuronal Types

A golgi study of the periaqueductal gray matter in the cat. Neuronal types and their distribution

1985 ◽  
Vol 58 (2) ◽  
Author(s):  
M. Gioia ◽  
G. Tredici ◽  
R. Bianchi
Keyword(s):  
Gray Matter ◽  
Periaqueductal Gray ◽  
Golgi Study ◽  
Periaqueductal Gray Matter ◽  
Neuronal Types

Neuronal Types in the Human Anterior Ventral Thalamic Nucleus: A Golgi Study

2007 ◽  
Vol 27 (6) ◽  
pp. 745-755 ◽  
Author(s):  
Saleh Al-Hussain Bani Hani ◽  
Mohammad Hassan Al-Haidari ◽  
Malik Mohammad Saboba
Keyword(s):  
Thalamic Nucleus ◽  
Golgi Study ◽  
Neuronal Types

A Golgi study of the monkey paraventricular nucleus: Neuronal types, afferent and efferent fibers

1987 ◽  
Vol 257 (4) ◽  
pp. 595-613 ◽  
Author(s):  
Jos� A. Rafols ◽  
Neil Aronin ◽  
Marian Difiglia
Keyword(s):  
Paraventricular Nucleus ◽  
Golgi Study ◽  
Neuronal Types

scholarly journals Calcitonin gene-related peptide regulates spinal microglial activation through the histone H3 lysine 27 trimethylation via enhancer of zeste homolog-2 in rats with neuropathic pain

2021 ◽  
Vol 18 (1) ◽  
Author(s):  
Qi An ◽  
Chenyan Sun ◽  
Ruidi Li ◽  
Shuhui Chen ◽  
Xinpei Gu ◽  
...  
Keyword(s):  
Neuropathic Pain ◽  
Dorsal Horn ◽  
Nerve Injury ◽  
Spinal Dorsal Horn ◽  
Microglial Activation ◽  
Microglial Cells ◽  
Gene Promoters ◽  
Spinal Dorsal ◽  
Related Peptide ◽  
Calcitonin Gene

Abstract Background Calcitonin gene-related peptide (CGRP) as a mediator of microglial activation at the transcriptional level may facilitate nociceptive signaling. Trimethylation of H3 lysine 27 (H3K27me3) by enhancer of zeste homolog 2 (EZH2) is an epigenetic mark that regulates inflammatory-related gene expression after peripheral nerve injury. In this study, we explored the relationship between CGRP and H3K27me3 in microglial activation after nerve injury, and elucidated the underlying mechanisms in the pathogenesis of chronic neuropathic pain. Methods Microglial cells (BV2) were treated with CGRP and differentially enrichments of H3K27me3 on gene promoters were examined using ChIP-seq. A chronic constriction injury (CCI) rat model was used to evaluate the role of CGRP on microglial activation and EZH2/H3K27me3 signaling in CCI-induced neuropathic pain. Results Overexpressions of EZH2 and H3K27me3 were confirmed in spinal microglia of CCI rats by immunofluorescence. CGRP treatment induced the increased of H3K27me3 expression in the spinal dorsal horn and cultured microglial cells (BV2) through EZH2. ChIP-seq data indicated that CGRP significantly altered H3K27me3 enrichments on gene promoters in microglia following CGRP treatment, including 173 gaining H3K27me3 and 75 losing this mark, which mostly enriched in regulation of cell growth, phagosome, and inflammation. qRT-PCR verified expressions of representative candidate genes (TRAF3IP2, BCL2L11, ITGAM, DAB2, NLRP12, WNT3, ADAM10) and real-time cell analysis (RTCA) verified microglial proliferation. Additionally, CGRP treatment and CCI increased expressions of ITGAM, ADAM10, MCP-1, and CX3CR1, key mediators of microglial activation in spinal dorsal horn and cultured microglial cells. Such increased effects induced by CCI were suppressed by CGRP antagonist and EZH2 inhibitor, which were concurrently associated with the attenuated mechanical and thermal hyperalgesia in CCI rats. Conclusion Our findings highly indicate that CGRP is implicated in the genesis of neuropathic pain through regulating microglial activation via EZH2-mediated H3K27me3 in the spinal dorsal horn.

scholarly journals A Golgi Study of Neurons in the Camel Cerebellum ( Camelus Dromedarius )

2021 ◽  
Author(s):  
Saleh M. Al‐Hussain ◽  
Mustafa S. Yousuf ◽  
Ayat Bani Hani ◽  
Sami Zaqout ◽  
Laiche Djouhri ◽  
...  
Keyword(s):  
Camelus Dromedarius ◽  
Golgi Study
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