Genotype-independent leaf disc transformation of potato (Solanum tuberosum) using Agrobacterium tumefaciens

1988 ◽  
Vol 76 (5) ◽  
pp. 767-774 ◽  
Author(s):  
M. De Block
Keyword(s):  
Solanum Tuberosum ◽  
Agrobacterium Tumefaciens ◽  
Leaf Disc ◽  
Leaf Disc Transformation

Leaf disc transformation of cultivated tomato (L. esculentum) using Agrobacterium tumefaciens

1986 ◽  
Vol 5 (2) ◽  
pp. 81-84 ◽  
Author(s):  
Sheila McCormick ◽  
Jeanne Niedermeyer ◽  
Joyce Fry ◽  
Arlene Barnason ◽  
Robert Horsch ◽  
...  
Keyword(s):  
Agrobacterium Tumefaciens ◽  
Leaf Disc ◽  
Leaf Disc Transformation

Establishment of an efficient Agrobacterium tumefaciens-mediated leaf disc transformation of Thellungiella halophila

2007 ◽  
Vol 26 (10) ◽  
pp. 1785-1789 ◽  
Author(s):  
Hong-Qing Li ◽  
Jie Xu ◽  
Lei Chen ◽  
Mei-Ru Li
Keyword(s):  
Agrobacterium Tumefaciens ◽  
Leaf Disc ◽  
Thellungiella Halophila ◽  
Leaf Disc Transformation

Regulation of Agrobacterium tumefaciens T-cyt gene expression in leaves of transgenic potato (Solanum tuberosum L. cv. Désirée) is strongly influenced by plant culture conditions

1991 ◽  
Vol 17 (4) ◽  
pp. 711-725 ◽  
Author(s):  
Dave Dymock ◽  
Ruth Risiott ◽  
Sylvia de Pater ◽  
Jane Lancaster ◽  
Penny Tillson ◽  
...  
Keyword(s):  
Gene Expression ◽  
Solanum Tuberosum ◽  
Agrobacterium Tumefaciens ◽  
Solanum Tuberosum L ◽  
Transgenic Potato ◽  
Culture Conditions ◽  
Plant Culture

Effects of hornonal balance during leaf-disc infection of Nicotiana plumbaginifolia with Agrobacterium tumefaciens nopaline strain C58

1988 ◽  
Vol 122 (3-4) ◽  
pp. 237-247
Author(s):  
Maddalena Querci ◽  
Elisabetta Lupotto
Keyword(s):  
Agrobacterium Tumefaciens ◽  
Leaf Disc ◽  
Nicotiana Plumbaginifolia

Inducción de variantes somaclonales y transformación en papa (Solanum tuberosum) empleando Agrobacterium tumefaciens

1991 ◽  
Vol 18 (3) ◽  
pp. 129-136
Author(s):  
Miguel Jordán ◽  
Carlos Reveraro ◽  
Andrea Stipo ◽  
Julio Aguirre ◽  
Luis Velásquez ◽  
...  
Keyword(s):  
Solanum Tuberosum ◽  
Agrobacterium Tumefaciens

Transformation of potato (Solanum tuberosum) cv. Mantiqueira using Agrobacterium tumefaciens and evaluation of herbicide resistance

1994 ◽  
Vol 13 (12) ◽  
Author(s):  
EliseuS. Figueira Filho ◽  
L�cioF.A. Figueiredo ◽  
DamaresC. Monte-Neshich
Keyword(s):  
Solanum Tuberosum ◽  
Agrobacterium Tumefaciens ◽  
Herbicide Resistance

scholarly journals Transformasi Genetik Tanaman Kentang (Solanum tuberosum L.) dengan Gen acvB Menggunakan Vektor Agrobacterium tumefaciens

2021 ◽  
Vol 11 (1) ◽  
pp. 63
Author(s):  
DARWIN SILALAHI ◽  
I GEDE PUTU WIRAWAN ◽  
MADE SRITAMIN
Keyword(s):  
Solanum Tuberosum ◽  
Agrobacterium Tumefaciens ◽  
Treatment Group ◽  
Genetic Transformation ◽  
Solanum Tuberosum L ◽  
Control Group ◽  
Shoot Height ◽  
Specific Pcr ◽  
Convenient Technique

Agrobacterium tumefaciens Mediated Genetic Transformation of acvB Gene in Potato (Solanum tuberosum L.). Genetic transformations are now routinely applied to plant mediated by Agrobacterium tumefaciens as the most convenient technique. This study aimed to prove the success of A. tumefaciens mediated genetic transformation in potato. A. tumefaciens LBA (pBI 121) and explant of potato shoot were used in this study. Explants were grown in vitro on Murashige and Skoog media. Transformation was implemented using smear technique by smearing A. tumefaciens to injured explant. Experimental groups consisted of two groups: control group which did not receive transformation treatment and treatment group receiving transformation treatment. Explant growth was observed through the presence of shoots, branches and the shoot height. Explants in the treatment group resulted in a higher number of shoots, branches, and shoot heights compared to control. Phenol compounds appear in explant epidermal tissue, indicating the wounds produced by A. tumefaciens infection, thus the gene predicted to be transformed. Identification by PCR is needed to prove the existence of the acvB gene in potato plants genome, using acvB specific PCR primer as the marker, such as (5?-CCCT CTAG AGAC CCGC GCCA AGGCG-3?) and (5?CGCG TCGA CCTT GTCG GAAAG -3?) with 540-bp in base pair size produced.

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