Regulation of Agrobacterium tumefaciens T-cyt gene expression in leaves of transgenic potato (Solanum tuberosum L. cv. Désirée) is strongly influenced by plant culture conditions

1991 ◽  
Vol 17 (4) ◽  
pp. 711-725 ◽  
Author(s):  
Dave Dymock ◽  
Ruth Risiott ◽  
Sylvia de Pater ◽  
Jane Lancaster ◽  
Penny Tillson ◽  
...  
Keyword(s):  
Gene Expression ◽  
Solanum Tuberosum ◽  
Agrobacterium Tumefaciens ◽  
Solanum Tuberosum L ◽  
Transgenic Potato ◽  
Culture Conditions ◽  
Plant Culture

scholarly journals Transcriptome analysis uncovers the gene expression profile of salt-stressed potato (Solanum tuberosum L.)

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Qing Li ◽  
Yuzhi Qin ◽  
Xinxi Hu ◽  
Guangcun Li ◽  
Hongying Ding ◽  
...  
Keyword(s):  
Gene Expression ◽  
Solanum Tuberosum ◽  
Gene Expression Profile ◽  
Expression Profile ◽  
Transcriptome Analysis ◽  
Solanum Tuberosum L

scholarly journals Transformasi Genetik Tanaman Kentang (Solanum tuberosum L.) dengan Gen acvB Menggunakan Vektor Agrobacterium tumefaciens

2021 ◽  
Vol 11 (1) ◽  
pp. 63
Author(s):  
DARWIN SILALAHI ◽  
I GEDE PUTU WIRAWAN ◽  
MADE SRITAMIN
Keyword(s):  
Solanum Tuberosum ◽  
Agrobacterium Tumefaciens ◽  
Treatment Group ◽  
Genetic Transformation ◽  
Solanum Tuberosum L ◽  
Control Group ◽  
Shoot Height ◽  
Specific Pcr ◽  
Convenient Technique

Agrobacterium tumefaciens Mediated Genetic Transformation of acvB Gene in Potato (Solanum tuberosum L.). Genetic transformations are now routinely applied to plant mediated by Agrobacterium tumefaciens as the most convenient technique. This study aimed to prove the success of A. tumefaciens mediated genetic transformation in potato. A. tumefaciens LBA (pBI 121) and explant of potato shoot were used in this study. Explants were grown in vitro on Murashige and Skoog media. Transformation was implemented using smear technique by smearing A. tumefaciens to injured explant. Experimental groups consisted of two groups: control group which did not receive transformation treatment and treatment group receiving transformation treatment. Explant growth was observed through the presence of shoots, branches and the shoot height. Explants in the treatment group resulted in a higher number of shoots, branches, and shoot heights compared to control. Phenol compounds appear in explant epidermal tissue, indicating the wounds produced by A. tumefaciens infection, thus the gene predicted to be transformed. Identification by PCR is needed to prove the existence of the acvB gene in potato plants genome, using acvB specific PCR primer as the marker, such as (5?-CCCT CTAG AGAC CCGC GCCA AGGCG-3?) and (5?CGCG TCGA CCTT GTCG GAAAG -3?) with 540-bp in base pair size produced.

scholarly journals Obtaining of transgenic potato (Solanum tuberosum L.) cultivar IPB CP3 containing LYZ‐C gene resistant to bacterial wilt disease

2021 ◽  
Vol 26 (1) ◽  
pp. 48
Author(s):  
Pasmawati Pasmawati ◽  
Aris Tjahjoleksono ◽  
Suharsono Suharsono
Keyword(s):  
Solanum Tuberosum ◽  
Bacterial Wilt ◽  
Solanum Tuberosum L ◽  
Transgenic Potato ◽  
Potato Production ◽  
Wilt Disease ◽  
Bacterial Disease ◽  
Gene Encoding ◽  
Bacterial Diseases ◽  
Bacterial Wilt Disease

Bacterial wilt caused by Ralstonia solanacearum is one of the most important bacterial diseases in potato production. This study aimed to obtain the transgenic potato (Solanum tuberosum L.) cultivar IPB CP3, containing LYZ‐C gene encoding for lysozyme type C, resistant to bacterial disease caused by R. solanacearum. Genetic transformation using Agrobacterium tumefaciens LBA4404 to 124 internode explants resulted in the transformation efficiency of about 47.58% with a regeneration efficiency of approximately 30.51%. Gene integration analysis showed that 16 clones were confirmed as transgenic clones containing the LYZ‐C gene. Analysis of resistance to R. solanacearum of three transgenic clones showed that all three transgenic clones were more resistant than a non‐transgenic one. This result showed that the LYZ‐C gene integrated in the genome of transgenic potato increased the resistance of potato plants to R. solanacearum. We obtained two transgenic clones considered resistant to bacterial wilt disease.

scholarly journals Efecto del ácido bórico y la luz en el número y biomasa de microtubérculos de papa cv. “Floresta”

Uniciencia ◽  
2017 ◽  
Vol 31 (2) ◽  
pp. 121
Author(s):  
José Antonio García-García ◽  
José Bernal Azofeifa-Bolaños
Keyword(s):  
Solanum Tuberosum ◽  
Boric Acid ◽  
Solanum Tuberosum L ◽  
Culture Conditions ◽  
Light Conditions ◽  
Lighting Conditions ◽  
Significant Difference ◽  
Murashige And Skoog Medium ◽  
Specific Culture

In order to understand the performance of Solanum tuberosum L. “Floresta” in producing microtubers, the effect of four concentrations of boric acid (6.2 mg l-1, 7.75 mg l-1, 9.3 mg l-1, and 10.85 mg l-1) under two conditions—1) 8 hour dark and 2) 16 hour light photoperiod and 8 hour dark—was evaluated under in vitro culture conditions. Full-strength Murashige and Skoog medium was used as a control. The data analysis for the number and biomass of microtubers were significant for the condition (p ≤ 0.05). Concerning the concentration of boric acid and the interaction between factors, a significant difference was obtained for biomass (p ≤ 0.05). In dark conditions, a greater number of microtubers was obtained, although the biomass was lower compared to the light conditions where the biomass was higher, and the number of microtubers was lower. A concentration of 9.3 mg l-1 of boric acid in both types of conditions was the best treatment to produce a greater number and more biomass of microtubers. The differences identified in this work with regards to the number and biomass of microtubers were probably the combined result of genotype and specific culture conditions. Even considering this, the use of lighting conditions is proposed to induce larger and greener microtubers.

scholarly journals Analysis of Cytosolic Heteroglycans from Leaves of Transgenic Potato (Solanum tuberosum L.) Plants that Under- or Overexpress the Pho 2 Phosphorylase Isozyme

2005 ◽  
Vol 46 (12) ◽  
pp. 1987-2004 ◽  
Author(s):  
Joerg Fettke ◽  
Simon Poeste ◽  
Nora Eckermann ◽  
Axel Tiessen ◽  
Markus Pauly ◽  
...  
Keyword(s):  
Solanum Tuberosum ◽  
Solanum Tuberosum L ◽  
Transgenic Potato
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