Latitudinal variation in octanol dehydrogenase and acid phosphatase allele frequencies in Drosophila melanogaster

1983 ◽  
Vol 65 (3) ◽  
pp. 191-196 ◽  
Author(s):  
J. G. Oakeshott ◽  
J. B. Gibson ◽  
D. A. Willcocks ◽  
G. K. Chambers



Development ◽  
1977 ◽  
Vol 39 (1) ◽  
pp. 45-57
Author(s):  
F. Giorgi ◽  
J. Jacob

The role played by the vitellogenic oocytes of Drosophila melanogaster in relation to the elaboration of material taken from the haemolymph is examined by ultrastructural cytochemistry. As revealed by the Gomori procedure, acid phosphatase occurs widely over the forming yolk platelets of the cortical and central ooplasm. A number of Golgi apparatuses in thecortical ooplasm are also positively stained with lead precipitates. With the proceeding of the ovarian development it becomes progressively more difficult to demonstrate cytochemically the enzyme over the yolk platelets. In stage 9–10 chambers the acid phosphatase is restricted to the so-called associated body, while the rest of the yolk platelet appears devoid of lead deposits. By using a osmium zinc iodide (OZ1) complex as a preferential staining method for the Golgi apparatus, it has been shown that, apart from the apparatus itself, a number of OZI deposits occur over the superficial layer of the forming yolk platelets. When mature yolk platelets are formed at later stages, the OZI deposits in the yolk platelets come to be restricted to the cap-like region of the superficial layer which contains the associated body. In vitellogenic oocytes, both the internal lining of the limiting membrane of the forming yolk platelets and the associated body of the mature yolk platelets react positively, to cytochemical methods to demonstrate carbohydrates. The present findings are interpreted as indicating the involvement of lysosomal enzymes in the process of maturation of the yolk material. The suggestion is also made that such an involvement is required to accomplish a selective hydrolysis of those blood proteins which have been taken in by vitellogenic oocytes along with yolk precursors.



1977 ◽  
Vol 26 (1) ◽  
pp. 201-215
Author(s):  
R.L. Tapp ◽  
A. Hockaday

The copper-containing granules in the mid-gut epithelium of larval Drosophila melanogaster were examined for acid phosphatase by combined histochemistry and energy-dispersive, X-ray microanalysis. After incubation, many of the granules were shown to contain simultaneously copper and sulphur (which are normal constituents), and lead and phosphorus (which are the detectable elements of the reaction product). Earlier work has been consolidated and extended and the evidence that the granules are formed as cytolysosomes is reviewed.







1972 ◽  
Vol 6 (2-3) ◽  
pp. 205-216 ◽  
Author(s):  
John B. Bell ◽  
Ross J. MacIntyre ◽  
Allan P. Olivieri




Evolution ◽  
1998 ◽  
Vol 52 (5) ◽  
pp. 1353 ◽  
Author(s):  
Ricardo B. R. Azevedo ◽  
Avis C. James ◽  
Jennie McCabe ◽  
L. Partridge




2010 ◽  
Vol 72 (1) ◽  
pp. 12-15 ◽  
Author(s):  
Andrea D. Plunkett ◽  
Lev Y. Yampolsky

We propose an experimental model suitable for demonstrating allele frequency change in Drosophila melanogaster populations caused by selection against an easily scorable conditional lethal, namely recessive flightless alleles such as apterous and vestigial. Homozygotes for these alleles are excluded from reproduction because the food source used to establish each generation is accessible only by flight. The observed dynamics of flightless-allele frequencies generally follows the theoretically predicted pattern, with slight deviation toward less intense selection. We also suggest observing selection against flight-independent visible marker alleles in the same population as a meaningful comparison. The proposed experiments can easily be scheduled within one semester, and the expected data provide ample opportunities for discussion of quantitative evolutionary patterns.



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