Localization of calcitonin gene-related peptide and islet amyloid polypeptide in the rat and mouse pancreas

1992 ◽  
Vol 269 (2) ◽  
pp. 315-322 ◽  
Author(s):  
Bo Ahr�n ◽  
Frank Sundler
Keyword(s):  
Islet Amyloid Polypeptide ◽  
Calcitonin Gene Related Peptide ◽  
Islet Amyloid ◽  
Mouse Pancreas ◽  
Related Peptide ◽  
Calcitonin Gene

A New Calcitonin-Receptor-like Sequence in Rat Pulmonary Blood Vessels

1993 ◽  
Vol 85 (4) ◽  
pp. 385-388 ◽  
Author(s):  
F. Njuki ◽  
C. G. Nicholl ◽  
A. Howard ◽  
J. C. W. Mak ◽  
P. J. Barnes ◽  
...  
Keyword(s):  
Blood Vessels ◽  
Vascular Tone ◽  
Islet Amyloid Polypeptide ◽  
Calcitonin Gene Related Peptide ◽  
Binding Activity ◽  
Calcitonin Receptor ◽  
Islet Amyloid ◽  
Related Peptide ◽  
Calcitonin Gene

1. Two rat clones have been isolated which are similar to known calcitonin-receptor sequences. One of these does not have the distribution expected of a calcitonin receptor. It is widely distributed, with extremely high levels of expression in the lung, where it is associated with the blood vessels. 2. This rat sequence may represent the receptor for calcitonin-gene-related peptide or islet amyloid polypeptide. Both have binding activity in the lung and are potent vasodilators. The gene represented by this sequence may therefore play an important role in the maintenance of vascular tone.

The Physiology of Calcitonin Gene?Related Peptide in the Islet Compared with That of Islet Amyloid Polypeptide (Amylin)

1992 ◽  
Vol 657 (1 Calcitonin Ge) ◽  
pp. 299-312 ◽  
Author(s):  
D. BRETHERTON-WATT ◽  
M. A. GHATEI ◽  
H. JAMAL ◽  
S. G. GILBEY ◽  
P. M. JONES ◽  
...  
Keyword(s):  
Islet Amyloid Polypeptide ◽  
Calcitonin Gene Related Peptide ◽  
Islet Amyloid ◽  
Related Peptide ◽  
Calcitonin Gene

Amylin, calcitonin gene-related peptide, and adrenomedullin: effects on thiazide receptor and calcium

1997 ◽  
Vol 272 (3) ◽  
pp. F410-F415 ◽  
Author(s):  
P. Blakely ◽  
D. A. Vaughn ◽  
D. D. Fanestil
Keyword(s):  
Rat Kidney ◽  
Ionic Composition ◽  
Islet Amyloid Polypeptide ◽  
Calcitonin Gene Related Peptide ◽  
Urinary Calcium ◽  
Calcium Excretion ◽  
Electrolyte Excretion ◽  
Islet Amyloid ◽  
Related Peptide ◽  
Calcitonin Gene

We previously reported that salmon calcitonin, but not rat calcitonin, increased renal thiazide receptor (TZR) density and decreased renal calcium [urinary calcium excretion (U(Caex))] in the rat. Since calcitonins, islet amyloid polypeptide (amylin), calcitonin-gene related peptide (CGRP), and adrenomedullin interact with a family of calcitonin-related receptors, we examined the effects of these peptides on 1) TZR density, as quantitated by binding of [3H]metolazone to renal membranes; 2) plasma ionic composition; and 3) urinary electrolyte excretion. Subcutaneous amylin both increased TZR density nearly twofold and decreased U(Caex), with maximal effects by 24 h. The decreased U(Caex) occurred with plasma amylin levels in the physiological range, whereas the increased TZR did not reach maximum even with plasma amylin >100 times above normal. Similar doses of adrenomedullin increased TZR density modestly but without effect on U(Caex), whereas CGRP did not alter TZR density and tended to increase U(Caex). We propose that U(Caex) and TZR density in the rat kidney are regulated by rat amylin but not by rat calcitonin.

Binding sites for islet amyloid polypeptide in mammalian lung: species variation and effects on adenylyl cyclase

1995 ◽  
Vol 73 (7) ◽  
pp. 1030-1036 ◽  
Author(s):  
Ranjev Bhogal ◽  
David M. Smith ◽  
Ali A. Owji ◽  
Stephen R. Bloom
Keyword(s):  
Guinea Pig ◽  
Adenylyl Cyclase ◽  
Binding Sites ◽  
Islet Amyloid Polypeptide ◽  
Calcitonin Gene Related Peptide ◽  
Cyclase Activity ◽  
Adenylyl Cyclase Activity ◽  
Islet Amyloid ◽  
Related Peptide ◽  
Calcitonin Gene

Islet amyloid polypeptide (IAPP) and calcitonin gene related peptide (CGRP) share a 47% sequence homology. IAPP can interact with adenylyl cyclase coupled CGRP receptors. We have examined [125I]IAPP binding in mouse, pig, and guinea pig lung membranes in competition with IAPP, CGRP, and CGRP(8–37). Three types of site were shown by order of potency: (i) mouse, IAPP > CGRP(8–37) [Formula: see text] CGRP; (ii) pig, CGRP > IAPP > CGRP(8–37); and (iii) guinea pig, CGRP = IAPP = CGRP(8–37). Chemical cross-linking of [125I]IAPP and [125I]CGRP binding sites in lung demonstrated that both sites had similar molecular weights in any one species but differed across species, i.e., mouse Mr = 70 000 and 98 000; pig Mr = 68 000, 56 000, and 47 000; and guinea pig Mr = 106 000 and 56 000. Adenylyl cyclase activity was stimulated by forskolin and AlCl3–NaF in rat, mouse, pig, and guinea pig membranes. Only in mouse and pig were CGRP and IAPP able to stimulate adenylyl cyclase activity. In mouse lung CGRP and IAPP stimulated adenylyl cyclase activity with EC50 values of 642 ± 222 nM (n = 4) and 325 ± 115 nM (n = 4), respectively. In pig lung membranes EC50 values were 5.7 ± 0.3 nM (n = 4) for CGRP and 1230 ± 1130 nM (n = 4) for IAPP. Thus IAPP either did not stimulate adenylyl cyclase activity in these lung membranes or did so with a low potency.Key words: islet amyloid polypeptide, amylin, calcitonin gene related peptide, lung, receptors.

Effects of islet amyloid polypeptide (amylin) and calcitonin gene-related peptide (CGRP) on glucose metabolism in the rat

1992 ◽  
Vol 15 (1) ◽  
pp. 63-69 ◽  
Author(s):  
Tomoyuki Morishita ◽  
Akinori Yamaguchi ◽  
Toshiyuki Yamatani ◽  
Akira Nakamura ◽  
Noriyuki Arima ◽  
...  
Keyword(s):  
Glucose Metabolism ◽  
Islet Amyloid Polypeptide ◽  
Calcitonin Gene Related Peptide ◽  
Islet Amyloid ◽  
Related Peptide ◽  
Calcitonin Gene
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