The activity of two heat shock loci of Drosophila hydei in tissue culture cells and salivary gland cells as analyzed by in situ hybridization of complementary DNA

Chromosoma ◽  
1979 ◽  
Vol 72 (3) ◽  
pp. 281-291 ◽  
Author(s):  
P. J. A. Sondermeijer ◽  
N. H. Lubsen
Keyword(s):  
Tissue Culture ◽  
Salivary Gland ◽  
In Situ Hybridization ◽  
Heat Shock ◽  
Complementary Dna ◽  
Culture Cells ◽  
Drosophila Hydei ◽  
Tissue Culture Cells ◽  
Salivary Gland Cells

p75, a polypeptide component of karyoskeletal protein-enriched fractions associated with transcriptionally active loci of Drosophila melanogaster polytene chromosomes

1988 ◽  
Vol 8 (5) ◽  
pp. 1877-1886
Author(s):  
B M Benton ◽  
S Berrios ◽  
P A Fisher
Keyword(s):  
Tissue Culture ◽  
Drosophila Melanogaster ◽  
Transcriptional Regulation ◽  
Salivary Gland ◽  
Indirect Immunofluorescence ◽  
Polytene Chromosomes ◽  
Nuclear Interior ◽  
Culture Cells ◽  
Tissue Culture Cells ◽  
Larval Salivary Gland

A 75-kilodalton polypeptide has been identified which copurifies with karyoskeletal protein-enriched fractions prepared from Drosophila melanogaster embryos. Results of indirect immunofluorescence experiments suggest that this protein, here designated p75, is primarily associated with puffed regions of larval salivary gland polytene chromosomes. In nonpolytenized Schneider 2 tissue culture cells, p75 appeared to be localized throughout the nuclear interior during interphase. In mitotic cells, p75 was redistributed diffusely. A possible role for karyoskeletal elements in transcriptional regulation is discussed.

scholarly journals Effect of heat shock on ribosome synthesis in Drosophila melanogaster.

1988 ◽  
Vol 8 (1) ◽  
pp. 91-95 ◽  
Author(s):  
J Bell ◽  
L Neilson ◽  
M Pellegrini
Keyword(s):  
Tissue Culture ◽  
Protein Synthesis ◽  
Drosophila Melanogaster ◽  
Heat Shock ◽  
Ribosomal Protein ◽  
Ribosomal Proteins ◽  
Culture Cells ◽  
Tissue Culture Cells ◽  
Ribosomal Protein Synthesis ◽  
Correct Processing

In Drosophila tissue culture cells, the synthesis of ribosomal proteins was inhibited by a 1-h 37 degrees C heat shock. Ribosomal protein synthesis was repressed to a greater extent than that of most other proteins synthesized by these cells at 25 degrees C. After a 1-h heat shock, when the cells were returned to 25 degrees C, the ribosomal proteins were much slower than most other 25 degrees C proteins to return to pre-heat shock levels of synthesis. Relative to one another, all the ribosomal proteins were inhibited and later recovered to normal levels of synthesis at the same rate and to the same extent. Unlike the ribosomal proteins, the precursor to the large rRNAs was continually synthesized during heat shock, although at a slightly reduced level, but was not processed. It was rapidly degraded, with a half-life of approximately 16 min. Pre-heat shock levels of synthesis, stability, and correct processing were restored only when ribosomal protein synthesis returned to at least 50% of that seen in non-heat-shocked cells.

A DNA damage-responsive Drosophila melanogaster gene is also induced by heat shock

1986 ◽  
Vol 6 (12) ◽  
pp. 4767-4769
Author(s):  
A A Vivino ◽  
M D Smith ◽  
K W Minton
Keyword(s):  
Dna Damage ◽  
Tissue Culture ◽  
Drosophila Melanogaster ◽  
Heat Shock ◽  
Uv Irradiation ◽  
Transcriptional Level ◽  
Restriction Map ◽  
Culture Cells ◽  
Tissue Culture Cells ◽  
Heat Shock Induction

A gene isolated by screening Drosophila melanogaster tissue culture cells for DNA damage regulation was also found to be regulated by heat shock. After UV irradiation or heat shock, induction is at the transcriptional level and results in the accumulation of a 1.0-kilobase polyadenylated transcript. The restriction map of the clone bears no resemblance to the known heat shock genes, which are shown to be uninduced by UV irradiation.

scholarly journals In situ Electron Microscopic Observation of Negatively Stained Tissue Culture Cells Contaminated with Mycoplasmas

Microbiology ◽  
1983 ◽  
Vol 129 (11) ◽  
pp. 3379-3384
Author(s):  
H. Takayama ◽  
T. Katsumoto ◽  
K. Ohno ◽  
A. Nakaso ◽  
A. Takagi ◽  
...  
Keyword(s):  
Tissue Culture ◽  
Microscopic Observation ◽  
Electron Microscopic Observation ◽  
Electron Microscopic ◽  
Culture Cells ◽  
Tissue Culture Cells

scholarly journals Visualization of the nucleus and nuclear envelope in situ by SEM in tissue culture cells

2007 ◽  
Vol 2 (5) ◽  
pp. 1180-1184 ◽  
Author(s):  
T D Allen ◽  
S A Rutherford ◽  
S Murray ◽  
F Gardiner ◽  
E Kiseleva ◽  
...  
Keyword(s):  
Tissue Culture ◽  
Nuclear Envelope ◽  
Culture Cells ◽  
Tissue Culture Cells

scholarly journals p75, a polypeptide component of karyoskeletal protein-enriched fractions associated with transcriptionally active loci of Drosophila melanogaster polytene chromosomes.

1988 ◽  
Vol 8 (5) ◽  
pp. 1877-1886 ◽  
Author(s):  
B M Benton ◽  
S Berrios ◽  
P A Fisher
Keyword(s):  
Tissue Culture ◽  
Drosophila Melanogaster ◽  
Transcriptional Regulation ◽  
Salivary Gland ◽  
Indirect Immunofluorescence ◽  
Polytene Chromosomes ◽  
Nuclear Interior ◽  
Culture Cells ◽  
Tissue Culture Cells ◽  
Larval Salivary Gland

A 75-kilodalton polypeptide has been identified which copurifies with karyoskeletal protein-enriched fractions prepared from Drosophila melanogaster embryos. Results of indirect immunofluorescence experiments suggest that this protein, here designated p75, is primarily associated with puffed regions of larval salivary gland polytene chromosomes. In nonpolytenized Schneider 2 tissue culture cells, p75 appeared to be localized throughout the nuclear interior during interphase. In mitotic cells, p75 was redistributed diffusely. A possible role for karyoskeletal elements in transcriptional regulation is discussed.

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