Classical swine fever virus diagnostics and vaccine production in insect cells

1996 ◽  
Vol 20 (1-3) ◽  
pp. 271-277 ◽  
Author(s):  
Marcel M. Hulst ◽  
Rob J. M. Moormann
Keyword(s):  
Classical Swine Fever Virus ◽  
Insect Cells ◽  
Classical Swine Fever ◽  
Fever Virus ◽  
Vaccine Production ◽  
Virus Diagnostics

scholarly journals Optimal Conditions for the Expression of Glycoprotein E2 of Classical Swine Fever Virus using Baculovirus in Insect Cells

2014 ◽  
Vol 29 (2) ◽  
pp. 207-213
Author(s):  
Sung Min Bae ◽  
Seung Hee Lee ◽  
Won Suk Kwak ◽  
Yong Oh Ahn ◽  
Tae Young Shin ◽  
...  
Keyword(s):  
Classical Swine Fever Virus ◽  
Insect Cells ◽  
Classical Swine Fever ◽  
Fever Virus ◽  
Optimal Conditions ◽  
Glycoprotein E2

Secretory pathway limits the enhanced expression of classical swine fever virus E2 glycoprotein in insect cells

2001 ◽  
Vol 86 (1) ◽  
pp. 31-38 ◽  
Author(s):  
Monique M. van Oers ◽  
Adri A.M. Thomas ◽  
Rob J.M. Moormann ◽  
Just M. Vlak
Keyword(s):  
Classical Swine Fever Virus ◽  
Secretory Pathway ◽  
Insect Cells ◽  
Classical Swine Fever ◽  
Fever Virus ◽  
E2 Glycoprotein ◽  
Enhanced Expression

The feasibility of a novel bioreactor for vaccine production of classical swine fever virus

Vaccine ◽  
2013 ◽  
Vol 31 (6) ◽  
pp. 867-872 ◽  
Author(s):  
Sheng-Chi Wu ◽  
Ming-Yi Liau ◽  
Ya-Ching Lin ◽  
Chong-Jun Sun ◽  
Chi-Ting Wang
Keyword(s):  
Classical Swine Fever Virus ◽  
Classical Swine Fever ◽  
Fever Virus ◽  
Vaccine Production

Glycoprotein E2 of Classical Swine Fever Virus: Expression in Insect Cells and Identification as a Ribonuclease

Virology ◽  
1994 ◽  
Vol 200 (2) ◽  
pp. 558-565 ◽  
Author(s):  
Marcel M. Hulst ◽  
Gary Himes ◽  
Ed Newbigin ◽  
Rob J.M. Moormann
Keyword(s):  
Classical Swine Fever Virus ◽  
Insect Cells ◽  
Classical Swine Fever ◽  
Fever Virus ◽  
Glycoprotein E2 ◽  
Virus Expression

scholarly journals Effect of N-glycosylation inhibition on the synthesis and processing of classical swine fever virus glycoproteins.

2007 ◽  
Vol 54 (4) ◽  
pp. 813-819 ◽  
Author(s):  
Jolanta Tyborowska ◽  
Ewa Zdunek ◽  
Bogusław Szewczyk
Keyword(s):  
Cell Culture ◽  
Classical Swine Fever Virus ◽  
Insect Cells ◽  
Classical Swine Fever ◽  
Fever Virus ◽  
Biologically Active ◽  
Complete Disappearance ◽  
Dimer Formation ◽  
Molecular Masses ◽  
Glycosylation Inhibition

Classical swine fever virus (CSFV) is often used as a surrogate model in molecular studies of the closely related hepatitis C virus. In this report we have examined the effect of the inhibition of glycosylation on the survival and maturation of CSFV. Viral glycoproteins (E(rns), E1, E2) form biologically active complexes - homo- and heterodimers, which are indispensable for viral life cycle. Those complexes are highly N-glycosylated. We studied the influence of N-glycosylation on dimer formation using E(rns) and E2 glycoproteins produced in insect cells after infection with recombinant baculoviruses. The glycoproteins were efficiently synthesized in insect cells, had similar molecular masses and formed dimers like their natural counterparts. Surprisingly, the addition of tunicamycin (an antibiotic which blocks early steps of glycosylation) to insect cell culture blocked not only dimer formation but it also led to an almost complete disappearance of E2 even in monomeric form. Tunicamycin did not exert a similar effect on the synthesis and formation of E(rns) dimers; the dimers were still formed, which suggests that E(rns) glycan chains are not necessary for dimer formation. We have also found that very low doses of tunicamycin (much lower than those used for blocking N-glycosylation) drastically reduced CSFV spread in SK6 (swine kidney) cell culture and the virus yield. These facts indicate that N-glycosylation inhibitors structurally similar to tunicamycin may be potential therapeutics for the inhibition of the spread of CSFV and related viruses.

ARFGAP1 binds to classical swine fever virus NS5A protein and enhances CSFV replication in PK-15 cells

2021 ◽  
Vol 255 ◽  
pp. 109034
Author(s):  
Liang Zhang ◽  
Mingxing Jin ◽  
Mengzhao Song ◽  
Shanchuan Liu ◽  
Tao Wang ◽  
...  
Keyword(s):  
Classical Swine Fever Virus ◽  
Classical Swine Fever ◽  
Fever Virus ◽  
Ns5a Protein

Genome Variability of Classical Swine Fever Virus during the 2018–2020 Epidemic in Japan

2021 ◽  
pp. 109128
Author(s):  
Tatsuya Nishi ◽  
Katsuhiko Fukai ◽  
Tomoko Kato ◽  
Kotaro Sawai ◽  
Takehisa Yamamoto
Keyword(s):  
Classical Swine Fever Virus ◽  
Classical Swine Fever ◽  
Fever Virus ◽  
Genome Variability

scholarly journals A Novel E2 Glycoprotein Subunit Marker Vaccine Produced in Plant Is Able to Prevent Classical Swine Fever Virus Vertical Transmission after Double Vaccination

Vaccines ◽  
2021 ◽  
Vol 9 (5) ◽  
pp. 418
Author(s):  
Youngmin Park ◽  
Yeonsu Oh ◽  
Miaomiao Wang ◽  
Llilianne Ganges ◽  
José Alejandro Bohórquez ◽  
...  
Keyword(s):  
Vertical Transmission ◽  
Classical Swine Fever Virus ◽  
Neutralizing Antibodies ◽  
Subunit Vaccine ◽  
Vaccine Dose ◽  
Classical Swine Fever ◽  
Fever Virus ◽  
Tissue Samples ◽  
Marker Vaccine ◽  
E2 Glycoprotein

The efficacy of a novel subunit vaccine candidate, based in the CSFV E2 glycoprotein produced in plants to prevent classical swine fever virus (CSFV) vertical transmission, was evaluated. A Nicotiana benthamiana tissue culture system was used to obtain a stable production of the E2-glycoprotein fused to the porcine Fc region of IgG. Ten pregnant sows were divided into three groups: Groups 1 and 2 (four sows each) were vaccinated with either 100 μg/dose or 300 μg/dose of the subunit vaccine at 64 days of pregnancy. Group 3 (two sows) was injected with PBS. Groups 1 and 2 were boosted with the same vaccine dose. At 10 days post second vaccination, the sows in Groups 2 and 3 were challenged with a highly virulent CSFV strain. The vaccinated sows remained clinically healthy and seroconverted rapidly, showing efficient neutralizing antibodies. The fetuses from vaccinated sows did not show gross lesions, and all analyzed tissue samples tested negative for CSFV replication. However, fetuses of non-vaccinated sows had high CSFV replication in tested tissue samples. The results suggested that in vaccinated sows, the plant produced E2 marker vaccine induced the protective immunogenicity at challenge, leading to protection from vertical transmission to fetuses.

Characterization of antibody responses against a neutralizing epitope on the glycoprotein E2 of classical swine fever virus

2008 ◽  
Vol 153 (8) ◽  
pp. 1593-1598 ◽  
Author(s):  
Y. Qi ◽  
L. C. Liu ◽  
B. Q. Zhang ◽  
Z. Shen ◽  
J. Wang ◽  
...  
Keyword(s):  
Classical Swine Fever Virus ◽  
Classical Swine Fever ◽  
Fever Virus ◽  
Antibody Responses ◽  
Neutralizing Epitope ◽  
Glycoprotein E2
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