Transmission electron microscopy observations of phagocytosis in trophozoites of Entamoeba histolytica in contact with tissue culture cells

1977 ◽  
Vol 52 (3) ◽  
pp. 203-211 ◽  
Author(s):  
T. F. McCaul
Keyword(s):  
Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Tissue Culture ◽  
Entamoeba Histolytica ◽  
Culture Cells ◽  
Tissue Culture Cells ◽  
Transmission Electron

Sulfhydryl bond formation is a prerequisite for proper cycling of centrosomes and chromosomes in mammalian tissue culture cells

1993 ◽  
Vol 51 ◽  
pp. 342-343
Author(s):  
Heide Schatten ◽  
Neidhard Paweletz ◽  
Ron Balczon
Keyword(s):  
Tissue Culture ◽  
Cell Cycle Progression ◽  
Group Formation ◽  
Sulfhydryl Group ◽  
Mammalian Tissue ◽  
Mitotic Chromosomes ◽  
Microtubule Network ◽  
Culture Cells ◽  
Tissue Culture Cells ◽  
Transmission Electron

To study the role of sulfhydryl group formation during cell cycle progression, mammalian tissue culture cells (PTK2) were exposed to 100¼M 2-mercaptoethanol for 2 to 6 h during their exponential phase of growth. The effects of 2-mercaptoethanol on centrosomes, chromosomes, microtubules, membranes and intermediate filaments were analyzed by transmission electron microscopy (TEM) and by immunofluorescence microscopy (IFM) methods using a human autoimmune antibody directed against centrosomes (SPJ), and a mouse monoclonal antibody directed against tubulin (E7). Chromosomes were affected most by this treatment: premature chromosome condensation was detected in interphase nuclei, and the structure in mitotic chromosomes was altered compared to control cells. This would support previous findings in dividing sea urchin cells in which chromosomes are arrested at metaphase while the centrosome splitting cycle continues. It might also support findings that certairt-sulfhydryl-blocking agents block cyclin destruction. The organization of the microtubule network was scattered probably due to a looser organization of centrosomal material at the interphase centers and at the mitotic poles.

Re-Embedding of Tissue Culture Cells for Comparative and Quantitative Electron Microscopy

1977 ◽  
Vol 35 ◽  
pp. 552-553
Author(s):  
P. S. Connelly
Keyword(s):  
Electron Microscopy ◽  
Tissue Culture ◽  
Comparative Study ◽  
Quantitative Study ◽  
Cell Monolayer ◽  
Quantitative Electron Microscopy ◽  
Culture Cells ◽  
Tissue Culture Cells ◽  
Harvesting Method ◽  
Number Of Cells

Three common methods of preparing tissue culture cells for examination by TEM, i.e. harvesting and pelleting, face-on sectioning and cross-sectioning have inherent limitations if the cells are to be used in a comparative and quantitative study. The large number of cells per section in the harvesting method is overshadowed by the total loss of initial orientation of the cells, changes in organelle position and damage to a number of the cells due to the disruptive methods utilized in the harvesting. Face-on sectioning, which is ideal for the examination of preselected individual cells has the limitation in a comparative study of varying section thicknesses and possible staining differences. These limitations also apply to crosssections and two additional factors add to the reasons why this method is usually rejected. First, unless the cell monolayer is confluent, there will be relatively few cells in a section and second, these sections cannot be examined on unsupported grids because the cells are on the edge of the section.

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