scholarly journals Cytoplasmic microtubular images in glutaraldehyde-fixed tissue culture cells by electron microscopy and by immunofluorescence microscopy

1978 ◽  
Vol 75 (4) ◽  
pp. 1820-1824 ◽  
Author(s):  
K. Weber ◽  
P. C. Rathke ◽  
M. Osborn
Keyword(s):  
Electron Microscopy ◽  
Tissue Culture ◽  
Immunofluorescence Microscopy ◽  
Fixed Tissue ◽  
Culture Cells ◽  
Tissue Culture Cells

Imaging of the Cytoskeleton Using Live and Fixed Tissue Culture Cells

2021 ◽  
pp. 159-173
Author(s):  
Derek A. Applewhite ◽  
Christine A. Lacy ◽  
Eric R. Griffis ◽  
Omar A. Quintero-Carmona
Keyword(s):  
Tissue Culture ◽  
Fixed Tissue ◽  
Culture Cells ◽  
Tissue Culture Cells

Re-Embedding of Tissue Culture Cells for Comparative and Quantitative Electron Microscopy

1977 ◽  
Vol 35 ◽  
pp. 552-553
Author(s):  
P. S. Connelly
Keyword(s):  
Electron Microscopy ◽  
Tissue Culture ◽  
Comparative Study ◽  
Quantitative Study ◽  
Cell Monolayer ◽  
Quantitative Electron Microscopy ◽  
Culture Cells ◽  
Tissue Culture Cells ◽  
Harvesting Method ◽  
Number Of Cells

Three common methods of preparing tissue culture cells for examination by TEM, i.e. harvesting and pelleting, face-on sectioning and cross-sectioning have inherent limitations if the cells are to be used in a comparative and quantitative study. The large number of cells per section in the harvesting method is overshadowed by the total loss of initial orientation of the cells, changes in organelle position and damage to a number of the cells due to the disruptive methods utilized in the harvesting. Face-on sectioning, which is ideal for the examination of preselected individual cells has the limitation in a comparative study of varying section thicknesses and possible staining differences. These limitations also apply to crosssections and two additional factors add to the reasons why this method is usually rejected. First, unless the cell monolayer is confluent, there will be relatively few cells in a section and second, these sections cannot be examined on unsupported grids because the cells are on the edge of the section.

scholarly journals Pre-embedding Double-Label Immunoelectron Microscopy of Chemically Fixed Tissue Culture Cells

2016 ◽  
pp. 217-232 ◽  
Author(s):  
Lou G. Boykins ◽  
Jonathan C. R. Jones ◽  
Carlos E. Estraño ◽  
Steven D. Schwartzbach ◽  
Omar Skalli
Keyword(s):  
Tissue Culture ◽  
Immunoelectron Microscopy ◽  
Fixed Tissue ◽  
Culture Cells ◽  
Tissue Culture Cells ◽  
Double Label

Crystalline Arrays of Foot-and-Mouth Disease Virus in Tissue Culture Cells

1967 ◽  
Vol 25 ◽  
pp. 102-103
Author(s):  
S.S. Breese ◽  
J.H. Graves
Keyword(s):  
Electron Microscopy ◽  
Tissue Culture ◽  
Disease Virus ◽  
Kidney Tissue ◽  
Foot And Mouth Disease ◽  
Bovine Kidney ◽  
Culture Cells ◽  
Tissue Culture Cells ◽  
Mouth Disease Virus ◽  
Swine Kidney

The formation of crystalline arrays of foot-and-mouth disease virus (FMDV) particles in swine kidney tissue culture cells has been examined in greater detail following the initial report of strain A119 in pig kidney cell line cultures, PK15. In the present experiments, FMDV strains recently isolated from field outbreaks in Argentina (A1, O2 and C3 CANEFA) were inoculated into primary swine kidney tissue cultures and samples at suitable intervals for embedding, sectioning and electron microscopy.The three strains of FMDV were stored at -20°C as the sixth bovine kidney tissue culture passage of original tongue tissue suspension. Immediately prior to use, they were inoculated into bovine kidney cultures and 1 ml of infected cell suspension was used in each swine kidney prescription bottle. After l/2 hr incubation at 37°C, 5 to 10 ml of maintenance medium was added and the bottles stored at 37°C. At intervals of 90 min, 3, 4, 5 and 6 hr after inoculation, bottles were processed for electron microscopy. Fluids were removed and the cells gently scraped from the glass into a few ml of Sorenson’s buffer at pH 7.2. Fixation was in 1% glutaraldehyde followed by 2% osmium tetroxide, dehydration, and embedment in epon. Sections were examined in an RCA-EMU-3-G microscope.

scholarly journals A STUDY OF TISSUE CULTURE CELLS BY ELECTRON MICROSCOPY

1945 ◽  
Vol 81 (3) ◽  
pp. 233-246 ◽  
Author(s):  
Keith R. Porter ◽  
Albert Claude ◽  
Ernest F. Fullam
Keyword(s):  
Electron Microscopy ◽  
Tissue Culture ◽  
Electron Micrographs ◽  
Cell Structure ◽  
Culture Technique ◽  
Chick Embryos ◽  
Tissue Culture Technique ◽  
Culture Cells ◽  
Tissue Culture Cells

By means of a tissue culture technique, cells from chick embryos were procured in a state which proved to be suitable for electron microscopy. The electron micrographs disclosed details of cell structure not revealed by other methods of examination.

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