Unstable alleles of the singed locus in Drosophila melanogaster with reference to a transposon marked with a visible mutation

1984 ◽  
Vol 194 (1-2) ◽  
pp. 279-285 ◽  
Author(s):  
Nicholas N. Yurchenko ◽  
Ilya K. Zakharov ◽  
Michael D. Golubovsky
Keyword(s):  
Drosophila Melanogaster ◽  
Unstable Alleles ◽  
Visible Mutation

scholarly journals High levels of fitness modifiers induced by hybrid dysgenesis in Drosophila melanogaster

1986 ◽  
Vol 48 (2) ◽  
pp. 89-94 ◽  
Author(s):  
Benjamin J. Fitzpatrick ◽  
John A. Sved
Keyword(s):  
Drosophila Melanogaster ◽  
Hybrid Dysgenesis ◽  
Wild Type ◽  
Homozygous Condition ◽  
Single Generation ◽  
Balancer Chromosome ◽  
Visible Mutation

SummaryWild-type chromosomes of D. melanogaster mutagenized by passage through a single generation of hybrid dysgenesis have been compared against identical chromosomes passed through a reciprocal, non-dysgenic cross. Fitness of the chromosome in homozygous condition has been examined in population cages using the technique of balancer chromosome equilibration. The results indicate that amongst chromosomes with no lethal or visible mutation, more than 50% have suffered a measurable decline in fitness. The magnitude of this decline is estimated to be in the range 10–20%.

A MUTATOR FACTOR IN A STRAIN OF DROSOPHILA MELANOGASTER: IDENTIFIED BY USE OF MUTATION, REVERSION RATES AND MALE RECOMBINATION

Genetics ◽  
1982 ◽  
Vol 101 (3-4) ◽  
pp. 417-429
Author(s):  
Nita N Scobie ◽  
Henry E Schaffer
Keyword(s):  
Drosophila Melanogaster ◽  
Mutation Accumulation ◽  
Female Sterility ◽  
Wild Type ◽  
Male Recombination ◽  
Male And Female ◽  
Male And Female Sterility ◽  
Visible Mutation

ABSTRACT A set of 1,000 "mutation accumulation" lines of Drosophila melanogaster, which originated from two different wild-type, lethal-bearing second chromosomes (Yamaguchi and Mukai 1974; Mukai and Cockerham 1977), was examined for evidence of a mutator factor by using the occurrence of recessive visible mutations and male recombination to identify its presence. The 1,000 lines were screened at approximately generation 240 for the presence of recessive visible mutations at twelve loci, by outcrossing to a balanced multiply marked second chromosome stock (Muller's "12ple" Bowling Green). Twenty-three lines were found to carry a visible mutation at one of the loci. Seventeen of these lines carried a mutation of either the dp or the vg locus. Mutations found in three lines, two at the dp locus and one at the vg locus, demonstrated instability as revertants to the wild type and were recovered and verified in these three cases. The three revertant lines, and three lines showing no reversion, were tested for their ability to induce male recombination. Male recombination was observed in the three lines in which revertants were recovered. Male and female sterility assays indicated conclusively that these "hybrid dysgenic" characteristics could not be used to identify lines potentially carrying mutator factors, whereas the consistent ability of the lines to induce high rates of reversion and male recombination was successful in determining that the "mutation accumulation lines" do possess mutator factors.

Author response for "Location and arrangement of campaniform sensilla in Drosophila melanogaster"

2020 ◽  
Author(s):  
Gesa F. Dinges ◽  
Alexander S. Chockley ◽  
Till Bockemühl ◽  
Kei Ito ◽  
Alexander Blanke ◽  
...  
Keyword(s):  
Drosophila Melanogaster ◽  
Author Response ◽  
Campaniform Sensilla

Live Confocal Analysis of Fertilization and Early Development

2001 ◽  
Vol 7 (S2) ◽  
pp. 1012-1013
Author(s):  
Uyen Tram ◽  
William Sullivan
Keyword(s):  
Drosophila Melanogaster ◽  
Embryonic Development ◽  
Real Time ◽  
Early Development ◽  
Fluorescent Probes ◽  
Primary Defect ◽  
Fluorescent Analysis ◽  
Dynamic Event ◽  
Enormous Amount ◽  
Fluorescent Studies

Embryonic development is a dynamic event and is best studied in live animals in real time. Much of our knowledge of the early events of embryogenesis, however, comes from immunofluourescent analysis of fixed embryos. While these studies provide an enormous amount of information about the organization of different structures during development, they can give only a static glimpse of a very dynamic event. More recently real-time fluorescent studies of living embryos have become much more routine and have given new insights to how different structures and organelles (chromosomes, centrosomes, cytoskeleton, etc.) are coordinately regulated. This is in large part due to the development of commercially available fluorescent probes, GFP technology, and newly developed sensitive fluorescent microscopes. For example, live confocal fluorescent analysis proved essential in determining the primary defect in mutations that disrupt early nuclear divisions in Drosophila melanogaster. For organisms in which GPF transgenics is not available, fluorescent probes that label DNA, microtubules, and actin are available for microinjection.

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