Studies on Fluorescent Analysis of Two Ethnomedicinal Plants Duranta erecta L. and Phyla nodiflora (L.) Greene

The fluorescent studies on two ethnomedicinal plants belongs to family Verbenaceae , Duranta erecta L. and Phyla nodiflora (L.) Greene. The present study will assist in standardization for quality, purity and sample identification. The etnomedicinal plants were analysed using standard methods.. The fluorescence analysis for two ethno-medicinal plants were conducted by using the visible light and ultra violet at 254nm and 354 nm, reveals the various colouration ranges from sea weed green to berry blue coloured highlighted compared with the source , Lularoe chart. The present study concludes that the data obtained can be used to authenticate, classify and standardize the above four ethno-medicinal plants. Keywords: Ethnomedicinal plants, fluorescent study, UV wavelength.

Schiff bases of 1,5-diarylpent-4-ene-1,3-diones and their metal complexes: Synthesis, characterization and fluorescent studies

Four Schiff bases (H2L1 to H2L4) have been synthesized by the con-densation between o-aminophenol and unsaturated diketones (1,5-diarylpent-4--ene-1,3-diones). Analytical, IR, 1H NMR and mass spectsral data revealed their existence in the imine-enamine form. Dibasic tetradentate coordination of the Schiff bases in their ML complexes [M = Cu(II), Ni(II), Co(II), Zn(II), Cd(II), and Hg(II)] has been established on the basis of physical, analytical and spectral data. The fluorescent studies of H2L4 show that fluorescence emission maxima shift with increase in polarity and hydrogen bonding ability of the solvent. Paramagnetic Cu(II), Ni(II) and Co(II) ions decrease the fluorescence intensity with increase in concentration of the metal ion while diamagnetic Zn(II), Cd(II) and Hg(II) ions have very little influence on the intensity of fluorescence of the Schiff base H2L4.

SPECTRAL-FLUORESCENT STUDIES OF EXOGENOUS FLUORESCENCE OF TOOTH TISSUES AFTER INTRA-CHANNEL ADMINISTRATION OF THE CHLORIN SERIES PHOTOSENSITIZER

The aim of current work was study of spreading chlorine photosentitizer in tooth tissues after its injection root canal. Study was held with help of local fluorescent spectroscopy method. Laser radiation with 408 nm letgth was used for stimulation of exogenic fluorescence of photosentitizer. After injection photosentitizer in root canal good spreading in microchannels and the whole volume of tooth was shown.

Isolation of RNA from a mixture and its detection by utilizing a microgel-based optical device

In this investigation, we show that RNA can be separated from a solution containing DNA and RNA and the isolated RNA can be detected using poly (N-isopropylacrylamide-co-N-(3-aminopropyl) methacrylamide hydrochloride) microgel-based optical devices (etalons). The isolation of RNA was accomplished by using hairpin-functionalized magnetic beads (MMPDNA) and differential melting, based on the fact that the DNA–RNA hybrid duplex is stronger (i.e., high melting temperature) than the DNA–DNA duplex (i.e., low melting temperature). By performing concurrent etalon sensing and fluorescent studies, we found that the MMPDNA combined with differential melting was capable of selectively separating RNA from DNA. This selective separation and simple colorimetric detection of RNA from a mixture will help lead to future RNA-based disease diagnostic devices.

From gold nanoparticles to luminescent nano-objects: experimental aspects for better gold-chromophore interactions

Gold nanoparticles have been the center of interest for scientists since many decades. Within the last 20 years, the research in that field has soared with the possibility to design and study nanoparticles with controlled shapes. From spheres to more complex shapes such as stars, or anisotropic architectures like rods or bipyramids, these new systems feature plasmonic properties making them the tools of choice for studies on light-matter interactions. In that context, fluorescence quenching and enhancement by gold nanostructures is a growing field of research. In this review, we report a non-exhaustive summary of the synthetic modes for various shapes and sizes of isotropic and anisotropic nanoparticles. We then focus on fluorescent studies of these gold nano-objects, either considering “bare” particles (without modifications) or hybrid particles (surface interaction with a chromophore). In the latter case, the well-known metal-enhanced fluorescence (MEF) is more particularly developed; the mechanisms of MEF are discussed in terms of the additional radiative and non-radiative decay rates caused by several parameters such as the vicinity of the chromophore to the metal or the size and shape of the nanostructures.