6-Methylguanine and 6-methylguanosine inhibit colony-forming ability in a malignant xeroderma pigmentosum cell line but not in other xeroderma pigmentosum and normal human fibroblast strains after treatment with 1-(2-chloroethyl)-1-nitroso-3-(2-hydroxyethyl)-urea

1987 ◽  
Vol 113 (1) ◽  
pp. 67-72 ◽  
Author(s):  
Heinz Walter Thielmann ◽  
Lutz Edler ◽  
Norbert M�ller ◽  
Gerhard Eisenbrand
Keyword(s):  
Cell Line ◽  
Human Fibroblast ◽  
Xeroderma Pigmentosum ◽  
Normal Human Fibroblast ◽  
Normal Human ◽  
Human Fibroblast Strains

Expression of P53 MRNA is roliferation-dependent in normal human fibroblast cell, line, WI-38

1995 ◽  
Vol 31 ◽  
pp. 383
Author(s):  
M. Kucsera ◽  
Z. Bori ◽  
J. Menyhárt ◽  
Z. Marcsek
Keyword(s):  
Cell Line ◽  
Human Fibroblast ◽  
Fibroblast Cell ◽  
Fibroblast Cell Line ◽  
Normal Human Fibroblast ◽  
Human Fibroblast Cell Line ◽  
Human Fibroblast Cell ◽  
Normal Human ◽  
P53 Mrna

Similar rate of O6-ethylguanine elimination from DNA in normal human fibroblast and xeroderma pigmentosum cell strains not transformed by SV40

1983 ◽  
Vol 4 (8) ◽  
pp. 1075-1077 ◽  
Author(s):  
Ian A. Teo ◽  
Alan R. Lehmann ◽  
Rolf Müller ◽  
Manfred F. Rajewsky
Keyword(s):  
Human Fibroblast ◽  
Xeroderma Pigmentosum ◽  
Similar Rate ◽  
Normal Human Fibroblast ◽  
Normal Human ◽  
Cell Strains

A Comparison of Ribonucleotide Reductase Activities in Normal Human Fibroblast Strains with Their Transformed Counterparts

Enzyme ◽  
1984 ◽  
Vol 32 (3) ◽  
pp. 142-148 ◽  
Author(s):  
Aaron Y. Tagger ◽  
Jim A. Wright
Keyword(s):  
Ribonucleotide Reductase ◽  
Human Fibroblast ◽  
Normal Human Fibroblast ◽  
Normal Human ◽  
Human Fibroblast Strains

Differential Activation of ERK 1/2 and JNK in Normal Human Fibroblast-like Cells in Response to UVC Radiation Under Different Oxygen Tensions¶

2000 ◽  
Vol 72 (3) ◽  
pp. 334 ◽  
Author(s):  
Naoki Matsuda ◽  
Miwa Horikawa ◽  
Li-Hong Wang ◽  
Masahiro Yoshida ◽  
Kumio Okaichi ◽  
...  
Keyword(s):  
Human Fibroblast ◽  
Normal Human Fibroblast ◽  
Differential Activation ◽  
Normal Human ◽  
Oxygen Tensions ◽  
Uvc Radiation

scholarly journals Phosphoprotein profiles of candidate markers for early cellular responses to low-dose γ-radiation in normal human fibroblast cells

2017 ◽  
Vol 58 (3) ◽  
pp. 329-340 ◽  
Author(s):  
Ji-Hye Yim ◽  
Jung Mi Yun ◽  
Ji Young Kim ◽  
In Kyung Lee ◽  
Seon Young Nam ◽  
...  
Keyword(s):  
Ionizing Radiation ◽  
Human Fibroblast ◽  
Low Dose ◽  
High Dose ◽  
Normal Human Fibroblast ◽  
Low Dose Radiation ◽  
Γ Radiation ◽  
Normal Human ◽  
Dose Radiation ◽  
In Cells

Abstract Ionizing radiation causes biological damage that leads to severe health effects. However, the effects and subsequent health implications caused by exposure to low-dose radiation are unclear. The objective of this study was to determine phosphoprotein profiles in normal human fibroblast cell lines in response to low-dose and high-dose γ-radiation. We examined the cellular response in MRC-5 cells 0.5 h after exposure to 0.05 or 2 Gy. Using 1318 antibodies by antibody array, we observed ≥1.3-fold increases in a number of identified phosphoproteins in cells subjected to low-dose (0.05 Gy) and high-dose (2 Gy) radiation, suggesting that both radiation levels stimulate distinct signaling pathways. Low-dose radiation induced nucleic acid–binding transcription factor activity, developmental processes, and multicellular organismal processes. By contrast, high-dose radiation stimulated apoptotic processes, cell adhesion and regulation, and cellular organization and biogenesis. We found that phospho-BTK (Tyr550) and phospho-Gab2 (Tyr643) protein levels at 0.5 h after treatment were higher in cells subjected to low-dose radiation than in cells treated with high-dose radiation. We also determined that the phosphorylation of BTK and Gab2 in response to ionizing radiation was regulated in a dose-dependent manner in MRC-5 and NHDF cells. Our study provides new insights into the biological responses to low-dose γ-radiation and identifies potential candidate markers for monitoring exposure to low-dose ionizing radiation.

scholarly journals Secretion of β-N-acetylglucosaminidase isoenzymes by normal human fibroblasts

1978 ◽  
Vol 173 (2) ◽  
pp. 433-439 ◽  
Author(s):  
P Willcox
Keyword(s):  
Lysosomal Enzyme ◽  
Human Fibroblast ◽  
Lysosomal Enzymes ◽  
Culture Medium ◽  
Human Fibroblasts ◽  
The Other ◽  
Normal Human Fibroblast ◽  
Acid Hydrolases ◽  
Human Fibroblast Cultures ◽  
Normal Human

1. Secretion of the lysosomal enzyme beta-N-acetylglucosaminidase (EC 3.2.1.30) by normal human fibroblast cultures was linear with respect to time up to 96h. 2. Two forms of the A isoenzyme of beta-N-acetylglucosaminidase were found in the culture medium. One form was similar to the isoenzyme found in other extracellular fluids, such as plasma and tears, the other resembled the intracellular (lysosomal) enzyme. The presence of the two isoenzymes in the culture medium appears to reflect two distinct secretory processes. 3. It is suggested that plasma acid hydrolases may be destined for incorporation into lysosomes in a manner analogous to that described for the packaging of lysosomal enzymes by fibroblasts.

scholarly journals Molecular mechanisms for the p38-induced cellular senescence in normal human fibroblast

2014 ◽  
Vol 156 (5) ◽  
pp. 283-290 ◽  
Author(s):  
Gakuro Harada ◽  
Qian Neng ◽  
Tsukasa Fujiki ◽  
Yoshinori Katakura
Keyword(s):  
Cellular Senescence ◽  
Human Fibroblast ◽  
Molecular Mechanisms ◽  
Normal Human Fibroblast ◽  
Normal Human
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