Relationship between asymmetrical and symmetrical hybrid DNA formation during meiotic recombination

1980 ◽  
Vol 1 (3) ◽  
pp. 185-191 ◽  
Author(s):  
J. -L. Rossignol ◽  
V. Haedens
Keyword(s):  
Meiotic Recombination ◽  
Hybrid Dna

Nonrandom transition from asymmetrical to symmetrical hybrid DNA during meiotic recombination

Genome ◽  
1989 ◽  
Vol 32 (3) ◽  
pp. 414-419 ◽  
Author(s):  
Angelos Kalogeropoulos ◽  
Jean-Luc Rossignol
Keyword(s):  
Meiotic Recombination ◽  
Heteroduplex Dna ◽  
Dna Distribution ◽  
Left Part ◽  
Ascobolus Immersus ◽  
Two Phases ◽  
The Right ◽  
Hybrid Dna

During meiotic recombination, in the b2 gene of Ascobolus immersus hybrid DNA can be formed either on only one (asymmetrical hybrid DNA) or on both (symmetrical hybrid DNA) interacting chromatids. The two phases can be found in the same meiosis, involving the same two interacting chromatids with the symmetrical phase located on the right with regard to the asymmetrical one. We show that the transition from the asymmetrical to the symmetrical phase occurs in a defined region located within the left part of the gene, which is closer to the initiation region. Once formed, the symmetrical hybrid DNA phase seems always to extend to the rightmost mutation sites. This contrasts with asymmetrical hybrid DNA extension, which when it stays in asymmetrical form, may stop within the gene.Key words: Ascobolus immersus, heteroduplex DNA distribution.

scholarly journals Hybrid DNA extension and reciprocal exchanges: alternative issues of an early intermediate during meiotic recombination?

Genetics ◽  
1988 ◽  
Vol 119 (2) ◽  
pp. 337-344
Author(s):  
T Langin ◽  
V Haedens ◽  
J L Rossignol
Keyword(s):  
Point Mutation ◽  
Meiotic Recombination ◽  
Point Mutations ◽  
Middle Part ◽  
The Other ◽  
High Conversion ◽  
Left Border ◽  
Mismatch Correction ◽  
The One ◽  
Hybrid Dna

Abstract Large heterologies in gene b2 strongly increase the frequencies of reciprocal exchanges on their left border, towards the high conversion end. In a previous study, we observed that heterozygous point mutations located in the high conversion end (region F) stimulate the reciprocal exchanges instigated by the large heterology 138. We have defined some properties of this stimulation. The effect does not depend on the nature of the large heterology used. It is effective only with point mutations located on the left side of the large heterology. It does not depend on the number of heterozygosities accumulated in region F. It is not specific on the location of point mutations in region F: it decreases from region F (left end) to region E (middle part of b2). It is correlated with the mismatch correction efficiencies of the point mutations used. It is not observed in the absence of a large heterology. Point mutation heterozygosities which stimulate reciprocal exchanges also decrease the frequency of HDNA formation in gene b2. We propose a model in which reciprocal exchanges on the one hand and hybrid DNA formation on the other hand correspond to alternative processings of a common recombination intermediate.

scholarly journals Hybrid DNA formation during meiotic recombination.

1981 ◽  
Vol 78 (12) ◽  
pp. 7648-7651 ◽  
Author(s):  
H. Hamza ◽  
V. Haedens ◽  
A. Mekki-Berrada ◽  
J. L. Rossignol
Keyword(s):  
Meiotic Recombination ◽  
Hybrid Dna

scholarly journals Meiotic mismatch repair quantified on the basis of segregation patterns in Schizosaccharomyces pombe.

Genetics ◽  
1993 ◽  
Vol 133 (4) ◽  
pp. 815-824 ◽  
Author(s):  
P Schär ◽  
P Munz ◽  
J Kohli
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Schizosaccharomyces Pombe ◽  
Detailed Analysis ◽  
Mismatch Repair ◽  
Meiotic Recombination ◽  
Wild Type ◽  
Base Pairs ◽  
Ascobolus Immersus ◽  
Postmeiotic Segregation ◽  
Hybrid Dna

Abstract Hybrid DNA with mismatched base pairs is a central intermediate of meiotic recombination. Mismatch repair leads either to restoration or conversion, while failure of repair results in postmeiotic segregation (PMS). The behavior of three G to C transversions in one-factor crosses with the wild-type alleles is studied in Schizosaccharomyces pombe. They lead to C/C and G/G mismatches and are compared with closely linked mutations yielding other mismatches. A method is presented for the detection of PMS in random spores. The procedure yields accurate PMS frequencies as shown by comparison with tetrad data. A scheme is presented for the calculation of the frequency of hybrid DNA formation and the efficiency of mismatch repair. The efficiency of C/C repair in S. pombe is calculated to be about 70%. Other mismatches are repaired with close to 100% efficiency. These results are compared with data published on mutations in Saccharomyces cerevisiae and Ascobolus immersus. This study forms the basis for the detailed analysis of the marker effects caused by G to C transversions in two-factor crosses.

scholarly journals Evidence for independent mismatch corrections along the same hybrid DNA tract during meiotic recombination in Ascobolus

Heredity ◽  
1980 ◽  
Vol 45 (2) ◽  
pp. 263-270 ◽  
Author(s):  
A Kalogeropoulos ◽  
J-L Rossignol
Keyword(s):  
Meiotic Recombination ◽  
Hybrid Dna

scholarly journals Rad51/Dmc1 paralogs and mediators oppose DNA helicases to limit hybrid DNA formation and promote crossovers during meiotic recombination

2014 ◽  
Vol 42 (22) ◽  
pp. 13723-13735 ◽  
Author(s):  
Alexander Lorenz ◽  
Alizée Mehats ◽  
Fekret Osman ◽  
Matthew C. Whitby
Keyword(s):  
Meiotic Recombination ◽  
Dna Helicases ◽  
Hybrid Dna

scholarly journals Hybrid DNA tracts may start at different sites during meiotic recombination in gene b2 of Ascobolus

1988 ◽  
Vol 7 (1) ◽  
pp. 253-259 ◽  
Author(s):  
A. Kalogeropoulos ◽  
J. L. Rossignol
Keyword(s):  
Meiotic Recombination ◽  
Hybrid Dna

Meiotic Recombination Protein SPO11

2020 ◽  
Author(s):  
Keyword(s):  
Meiotic Recombination ◽  
Recombination Protein
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