An evolutionary comparison of homologous mitochondrial plasmid DNAs from three Neurospora species

1985 ◽  
Vol 201 (2) ◽  
pp. 161-167 ◽  
Author(s):  
John W. Taylor ◽  
Beverly D. Smolich ◽  
Georgiana May
Keyword(s):  
Mitochondrial Plasmid ◽  
Plasmid Dnas ◽  
Evolutionary Comparison

Origin and direction of replication in mitochondrial plasmid DNAs of broad bean,Vicia faba

1988 ◽  
Vol 14 (2) ◽  
pp. 163-170 ◽  
Author(s):  
Jill A. Wahleithner ◽  
David R. Wolstenholme
Keyword(s):  
Vicia Faba ◽  
Broad Bean ◽  
Mitochondrial Plasmid ◽  
Plasmid Dnas

scholarly journals The Functions of the Multiproduct and Rapidly Evolving dec-1 Eggshell Gene Are Conserved Between Evolutionarily Distant Species of Drosophila

Genetics ◽  
2001 ◽  
Vol 159 (3) ◽  
pp. 1089-1102
Author(s):  
James C Badciong ◽  
Jeffery M Otto ◽  
Gail L Waring
Keyword(s):  
Biological Significance ◽  
Extracellular Proteins ◽  
Female Fertility ◽  
Open Reading Frames ◽  
Amino Acid Variability ◽  
Normal Expression ◽  
Evolutionary Comparison ◽  
Phenotypic Rescue ◽  
Conserved Amino Acids ◽  
Reading Frames

Abstract The Drosophila dec-1 gene encodes multiple proteins that are required for female fertility and proper eggshell morphogenesis. Genetic and immunolocalization data suggest that the different DEC-1 proteins are functionally distinct. To identify regions within the proteins with potential biological significance, we cloned and sequenced the D. yakuba and D. virilis dec-1 homologs. Interspecies comparisons of the predicted translation products revealed rapidly evolving sequences punctuated by blocks of conserved amino acids. Despite extensive amino acid variability, the proteins produced by the different dec-1 homologs were functionally interchangeable. The introduction of transgenes containing either the D. yakuba or the D. virilis dec-1 open reading frames into a D. melanogaster DEC-1 protein null mutant was sufficient to restore female fertility and wild-type eggshell morphology. Normal expression and extracellular processing of the DEC-1 proteins was correlated with the phenotypic rescue. The nature of the conserved features highlighted by the evolutionary comparison and the molecular resemblance of some of these features to those found in other extracellular proteins suggests functional correlates for some of the multiple DEC-1 derivatives.

Repair of heteroduplex plasmid DNA after transformation into Saccharomyces cerevisiae

1986 ◽  
Vol 6 (10) ◽  
pp. 3401-3409
Author(s):  
D K Bishop ◽  
R D Kolodner
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Plasmid Dna ◽  
Base Pair ◽  
Base Pairs ◽  
Plasmid Dnas ◽  
Repair Efficiency ◽  
Single Insertion ◽  
Base Pair Insertion

Purified heteroduplex plasmid DNAs containing 8- or 12-base-pair insertion mismatches or AC or CT substitution mismatches were used to transform Saccharomyces cerevisiae. Two insertion mismatches, separated by 943 base pairs, were repaired independently of each other at least 55% of the time. This suggested that repair tracts were frequently shorter than 1 kilobase. The two insertion mismatches were repaired with different efficiencies. Comparison of the repair efficiency of one mismatched site with or without an adjacent mismatch suggests that mismatches promote their own repair and can influence the repair of neighboring mismatches. When two different plasmids containing single-insertion mismatches were transformed into S. cerevisiae cells, a slight preference towards insertion was detected among repair products of one of the two plasmids, while no repair preference was detected among transformants with the second plasmid.

Genes Leave Their Characteristic Marks on the Melting Profile of Plasmid DNAs

1995 ◽  
Vol 50 (9-10) ◽  
pp. 652-655 ◽  
Author(s):  
Igor Z. Zubrzycki ◽  
Horst H. Klump
Keyword(s):  
High Resolution ◽  
Resistance Gene ◽  
High Resolution Melting ◽  
Uv Absorbance ◽  
Melting Profile ◽  
Plasmid Dnas ◽  
Parental Plasmid

Abstract High resolution melting profiles of four linearised plasmids (pUC9, pGV403, pHP2, and pBR322) were recorded by means of UV absorbance vs. teperature scaning. The set of transitions obtained for each plasmid are compared to each another and to the transitions obtained for their excised particular antibiotica resistance gene. It can be shown that each gene leaves a charactersitic mark on the melting profile of its parental plasmid

Comparison of the ability of viral protein-expressing plasmid DNAs to protect against influenza

Vaccine ◽  
1998 ◽  
Vol 16 (16) ◽  
pp. 1544-1549 ◽  
Author(s):  
Ze Chen ◽  
Yasuhiro Sahashi ◽  
Kazutoshi Matsuo ◽  
Hideki Asanuma ◽  
Hidehiro Takahashi ◽  
...  
Keyword(s):  
Viral Protein ◽  
Plasmid Dnas
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