Determination of the isoelectric point of a purified milk clotting enzyme preparation by analytical and preparative isoelectric focusing

1976 ◽  
Vol 279 (2) ◽  
pp. 118-119 ◽  
Author(s):  
K. Pozs�r-Hajnal
Keyword(s):  
Isoelectric Focusing ◽  
Isoelectric Point ◽  
Enzyme Preparation ◽  
Milk Clotting ◽  
Milk Clotting Enzyme

Isoelectric Point Determination of Live Polioviruses by Capillary Isoelectric Focusing with Whole Column Imaging Detection

2013 ◽  
Vol 85 (12) ◽  
pp. 6089-6094 ◽  
Author(s):  
Yvonne E. Thomassen ◽  
Gerco van Eikenhorst ◽  
Leo A. van der Pol ◽  
Wilfried A. M. Bakker
Keyword(s):  
Isoelectric Focusing ◽  
Isoelectric Point ◽  
Capillary Isoelectric Focusing ◽  
Imaging Detection ◽  
Point Determination

A multivariate approach for the determination of isoelectric point of human carbonic anhydrase isoforms by capillary isoelectric focusing

2011 ◽  
Vol 32 (20) ◽  
pp. 2857-2866 ◽  
Author(s):  
Marie Lecoeur ◽  
Jean-François Goossens ◽  
Claude Vaccher ◽  
Jean-Paul Bonte ◽  
Catherine Foulon
Keyword(s):  
Carbonic Anhydrase ◽  
Isoelectric Focusing ◽  
Isoelectric Point ◽  
Capillary Isoelectric Focusing ◽  
Multivariate Approach ◽  
Human Carbonic Anhydrase

scholarly journals Continuous on-chip fluorescence labelling, free-flow isoelectric focusing and marker-free isoelectric point determination of proteins and peptides

Lab on a Chip ◽  
2016 ◽  
Vol 16 (9) ◽  
pp. 1565-1572 ◽  
Author(s):  
Christin Herzog ◽  
Elisabeth Poehler ◽  
Andrea J. Peretzki ◽  
Sergey M. Borisov ◽  
Daniel Aigner ◽  
...  
Keyword(s):  
Real Time ◽  
Isoelectric Focusing ◽  
Isoelectric Point ◽  
Free Flow ◽  
Microfluidic Chips ◽  
Marker Free ◽  
Point Determination ◽  
On Chip ◽  
Proteins And Peptides

Presented are microfluidic chips that allow for continuous subsequent biomolecular labelling, free-flow isoelectric focusing and real-time isoelectric point monitoring.

scholarly journals Characteristics of Curds With Milk Clotting Enzyme from Indonesian Local Isolate of Lactic Acid Bacteria

2020 ◽  
Vol 1 (2) ◽  
pp. 79-86
Author(s):  
Wendry Putranto ◽  
Apon Mustopa ◽  
Jendri Mamangkey ◽  
Netty Aritonang
Keyword(s):  
Enzyme Activity ◽  
Ammonium Sulfate ◽  
Observational Research ◽  
Ammonium Sulfate Precipitation ◽  
Fermentation Time ◽  
Milk Clotting ◽  
Milk Clotting Enzyme ◽  
Qualitative Observation ◽  
Milk Clotting Activity

To get the potential of lalcat acid bacteria isolate to produce Milk Clotting Enzyme (MCE), it is necessary to screen milk clotting activity both quantitatively and qualitatively. Through qualitative observation, the characteristics of the curd resulting from enzyme activity can be obtained. MCE is a protease that has the characteristics of milking. Based on the results of this observational research, the curd characteristic produced can be used as a benchmark to determine the length of time of fermentation and optimization of the determination of ammonium sulfate precipitation concentration. Isolate BAL shows the results of a compact curd at a fermentation time of 25 hours at 37 ℃ and the optimization results of the deposition of ammonium sulfate which shows the characteristics of a compact curd by 45% ammonium sulfate.

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