Structure and expression of a split chloroplast gene from mustard (Sinapis alba): ribosomal protein gene rps16 reveals unusual transcriptional features and complex RNA maturation

1989 ◽  
Vol 15 (1) ◽  
pp. 63-70 ◽  
Author(s):  
Heike Neuhaus ◽  
Andrea Scholz ◽  
Gerhard Link
Keyword(s):  
Ribosomal Protein ◽  
Protein Gene ◽  
Ribosomal Protein Gene ◽  
Sinapis Alba ◽  
Chloroplast Gene ◽  
Rna Maturation

scholarly journals Isolation and structural analysis of a ribosomal protein gene inD.melanogaster

1988 ◽  
Vol 16 (11) ◽  
pp. 4915-4926 ◽  
Author(s):  
F. Rafti ◽  
G. Gargiulo ◽  
A. Manzi ◽  
C. Malva ◽  
G. Grossi ◽  
...  
Keyword(s):  
Structural Analysis ◽  
Ribosomal Protein ◽  
Protein Gene ◽  
Ribosomal Protein Gene

scholarly journals Isolation and characterisation of a murine cDNA clone highly homologous to the yeast L29 ribosomal protein gene

1987 ◽  
Vol 15 (3) ◽  
pp. 1019-1029 ◽  
Author(s):  
Pierre Belhumeur ◽  
Gary D. Paterno ◽  
Guy Boileau ◽  
Jean-Michel Claverie ◽  
Daniel Skup
Keyword(s):  
Ribosomal Protein ◽  
Cdna Clone ◽  
Protein Gene ◽  
Ribosomal Protein Gene

scholarly journals Mutations in the ribosomal protein gene RPL10 suggest a novel modulating disease mechanism for autism

2006 ◽  
Vol 11 (12) ◽  
pp. 1073-1084 ◽  
Author(s):  
S M Klauck ◽  
B Felder ◽  
A Kolb-Kokocinski ◽  
C Schuster ◽  
A Chiocchetti ◽  
...  
Keyword(s):  
Ribosomal Protein ◽  
Protein Gene ◽  
Ribosomal Protein Gene ◽  
Disease Mechanism

Association of RAP1 binding sites with stringent control of ribosomal protein gene transcription in Saccharomyces cerevisiae

1991 ◽  
Vol 11 (5) ◽  
pp. 2723-2735 ◽  
Author(s):  
C M Moehle ◽  
A G Hinnebusch
Keyword(s):  
Gene Expression ◽  
Saccharomyces Cerevisiae ◽  
Amino Acid ◽  
Ribosomal Protein ◽  
Binding Sites ◽  
Protein Gene ◽  
Ribosomal Protein Gene ◽  
Ribosomal Protein Genes ◽  
Biosynthetic Genes ◽  
Stringent Control

An amino acid limitation in bacteria elicits a global response, called stringent control, that leads to reduced synthesis of rRNA and ribosomal proteins and increased expression of amino acid biosynthetic operons. We have used the antimetabolite 3-amino-1,2,4-triazole to cause histidine limitation as a means to elicit the stringent response in the yeast Saccharomyces cerevisiae. Fusions of the yeast ribosomal protein genes RPL16A, CRY1, RPS16A, and RPL25 with the Escherichia coli lacZ gene were used to show that the expression of these genes is reduced by a factor of 2 to 5 during histidine-limited exponential growth and that this regulation occurs at the level of transcription. Stringent regulation of the four yeast ribosomal protein genes was shown to be associated with a nucleotide sequence, known as the UASrpg (upstream activating sequence for ribosomal protein genes), that binds the transcriptional regulatory protein RAP1. The RAP1 binding sites also appeared to mediate the greater ribosomal protein gene expression observed in cells growing exponentially than in cells in stationary phase. Although expression of the ribosomal protein genes was reduced in response to histidine limitation, the level of RAP1 DNA-binding activity in cell extracts was unaffected. Yeast strains bearing a mutation in any one of the genes GCN1 to GCN4 are defective in derepression of amino acid biosynthetic genes in 10 different pathways under conditions of histidine limitation. These Gcn- mutants showed wild-type regulation of ribosomal protein gene expression, which suggests that separate regulatory pathways exist in S. cerevisiae for the derepression of amino acid biosynthetic genes and the repression of ribosomal protein genes in response to amino acid starvation.

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