Comparative structural characterization of material extractable from aerial sheath of Streptomyces roseoflavus var. roseofungini

1974 ◽  
Vol 101 (1) ◽  
pp. 71-82 ◽  
Author(s):  
Natalia E. Cherny ◽  
Anna S. Tikhonenko ◽  
L. V. Kalakoutskii
2016 ◽  
Vol 425 ◽  
pp. 48-58 ◽  
Author(s):  
Sander S. van Leeuwen ◽  
Bas J.H. Kuipers ◽  
Lubbert Dijkhuizen ◽  
Johannis P. Kamerling

Tetrahedron ◽  
2000 ◽  
Vol 56 (35) ◽  
pp. 6427-6433 ◽  
Author(s):  
Susana Rojas-Lima ◽  
Norberto Farfán ◽  
Rosa Santillan ◽  
Dolores Castillo ◽  
Martha E. Sosa-Torres ◽  
...  

2017 ◽  
Vol 65 (39) ◽  
pp. 8744-8753 ◽  
Author(s):  
Ping-Ping Wang ◽  
Xin-Sheng Qin ◽  
Qing-Yu Yang ◽  
Zhi-Gang Luo ◽  
Zhi-Gang Xiao ◽  
...  

Author(s):  
S. F. Hayes ◽  
M. D. Corwin ◽  
T. G. Schwan ◽  
D. W. Dorward ◽  
W. Burgdorfer

Characterization of Borrelia burgdorferi strains by means of negative staining EM has become an integral part of many studies related to the biology of the Lyme disease organism. However, relying solely upon negative staining to compare new isolates with prototype B31 or other borreliae is often unsatisfactory. To obtain more satisfactory results, we have relied upon a correlative approach encompassing a variety EM techniques, i.e., scanning for topographical features and cryotomy, negative staining and thin sectioning to provide a more complete structural characterization of B. burgdorferi.For characterization, isolates of B. burgdorferi were cultured in BSK II media from which they were removed by low speed centrifugation. The sedimented borrelia were carefully resuspended in stabilizing buffer so as to preserve their features for scanning and negative staining. Alternatively, others were prepared for conventional thin sectioning and for cryotomy using modified procedures. For thin sectioning, the fixative described by Ito, et al.


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