Demonstration of 2n spermatids in carriers of the ?sex reversed? factor in the mouse by feulgen cytophotometry

1980 ◽  
Vol 69 (3) ◽  
pp. 299-306 ◽  
Author(s):  
E. Hannappel ◽  
W. Siegler ◽  
U. Drews
Keyword(s):  
Feulgen Cytophotometry

scholarly journals TWO-WAVE-LENGTH-SCANNING METHOD IN FEULGEN CYTOPHOTOMETRY

1972 ◽  
Vol 5 (3) ◽  
pp. 146-152 ◽  
Author(s):  
SETSUYA FUJITA ◽  
MASARU FUKUDA ◽  
TADAHISA KITAMURA ◽  
SATORU YOSHIDA
Keyword(s):  
Wave Length ◽  
Scanning Method ◽  
Feulgen Cytophotometry

Feulgen Cytophotometry of Pine Nuclei; Effects of Fixation, Role of Formaline

1968 ◽  
Vol 43 (2) ◽  
pp. 111-117 ◽  
Author(s):  
M. S. Greenwood ◽  
G. P. Berlyn
Keyword(s):  
Feulgen Cytophotometry

scholarly journals PRIMITIVE ERYTHROPOIESIS IN EARLY CHICK EMBRYOGENESIS

1971 ◽  
Vol 50 (3) ◽  
pp. 669-681 ◽  
Author(s):  
G. Le M. Campbell ◽  
H. Weintraub ◽  
B. H. Mayall ◽  
H. Holtzer
Keyword(s):  
Cell Cycle ◽  
Progressive Increase ◽  
Cell Cycles ◽  
Proliferative Phase ◽  
Cell Divisions ◽  
Chick Embryogenesis ◽  
Interphase Cells ◽  
Feulgen Cytophotometry ◽  
Successive Generations ◽  
Primitive Erythropoiesis

Primitive erythroblasts in the circulating blood of the chick embryo continue to divide while synthesizing hemoglobin (Hb). Hb measurements on successive generations of erythroblasts show that there is a progressive increase in the Hb content of both interphase and metaphase cells. Furthermore, for any given embryo the Hb content of metaphase cells is always significantly greater than that of interphase cells. The distribution of Hb values for metaphase cells suggests that there are six Hb classes corresponding to the number of cell cycles in the proliferative phase. The location of erythroblasts in the cell cycle was determined by combining Feulgen cytophotometry with thymidine radioautography on the same cells. Measurements of the Hb content for erythroblasts in different compartments of the cell cycle (G1, S, G2, and M) show a progressive increase through the cycle. Thus, the amount of Hb per cell is a function of the number of cell divisions since the initiation of Hb synthesis and, to a lesser degree, the stage of the cell cycle. Earlier generations of erythroblasts synthesize Hb at a faster rate than the terminal generation. Several models have been proposed to explain these findings.

Chromosomal alterations and DNA content in rats during ageing

Genome ◽  
1989 ◽  
Vol 32 (3) ◽  
pp. 389-392 ◽  
Author(s):  
Sarmistha Sen ◽  
Geeta Talukder ◽  
Archana Sharma
Keyword(s):  
Bone Marrow ◽  
Dna Content ◽  
Bone Marrow Cells ◽  
Age Groups ◽  
Chromosome Analysis ◽  
Male Rats ◽  
Age Related ◽  
Significant Difference ◽  
Feulgen Cytophotometry

Chromosome analysis of bone marrow cells from 96 sex-matched Rattus norvegicus of 12 different age groups showed a significant increase in hypodiploid cells with ageing. However, in meiotic preparations from gonadal cells of male rats the frequency of hypodiploid or hyperdiploid cells did not change significantly. In situ DNA estimation of bone marrow nuclei, following Feulgen cytophotometry, also did not show any significant difference with gradual age changes.Key words: senescence, DNA content, age-related changes.

Feulgen Cytophotometry of Pine Nuclei. II. Effect of Pectinase Used in Cell Sfparation

1979 ◽  
Vol 54 (4) ◽  
pp. 201-204 ◽  
Author(s):  
Graeme P. Berlyn ◽  
Sukhraj S. Dhillon ◽  
Jerome P. Miksche
Keyword(s):  
Feulgen Cytophotometry

DNA content of cervical intraepithelial neoplasia studied by two-wavelength Feulgen cytophotometry

1967 ◽  
Vol 98 (6) ◽  
pp. 792-799 ◽  
Author(s):  
George D. Wilbanks ◽  
Ralph M. Richart ◽  
Jacob Y. Terner
Keyword(s):  
Cervical Intraepithelial Neoplasia ◽  
Dna Content ◽  
Intraepithelial Neoplasia ◽  
Feulgen Cytophotometry

scholarly journals A different level of X-chromosomal transcription in an In(1)BM2 (reinverted) strain and in its hyperploid derivatives resolves an X-coded regulatory activity for dosage compensation in Drosophila

1986 ◽  
Vol 48 (2) ◽  
pp. 65-75 ◽  
Author(s):  
A. S. Mukherjee ◽  
Mita Ghosh
Keyword(s):  
Dosage Compensation ◽  
Rna Synthesis ◽  
Normal Male ◽  
Temperature Sensitive ◽  
Gene Complex ◽  
Regulatory Activity ◽  
Hypomorphic Mutation ◽  
Feulgen Cytophotometry ◽  
Mutant Male ◽  
Mutant Chromosome

SummaryThe transcriptional competence of the X-chromosome of a mutant strain of Drosophila melanogaster, [in(1)BM2 (reinverted)], and of hyperploid derivatives with different additional segments of the X-chromosome has been examined. The single X in the mutant male shows twice as much puffiness and RNA synthesis as does that in the normal male, revealing a level of X-coded activity in addition to the normal male and female levels. Feulgen cytophotometry reveals no duplication of DNA content in the mutant X. When duplication for the segments 1A-3E, 9A-20F, 11A-20F and 16A-20F of the X-chromosome are combined in the male with the mutant chromosome, the super-hyperactivity of the mutant X is completely abolished. In combination with the Bs. Y duplication, which contains 16A7-B2, the two-fold activity is also completely suppressed.The mutant chromosome can appear in three discrete manifestations, namely, highly flabby, intermittently flabby and normal, suggesting a leaky nature of the mutant. The effect is also temperature-sensitive. Our results suggest that there may be a modulator gene complex (M+) in the 16A7-B2 region as well as regulators elsewhere on the X, which in combination influence the hyperactivity of the male X in Drosophila. We suggest that the In(1)BM2 (reinverted) chromosome carries a hypomorphic mutation of M+(Mm). The results presented here and earlier data on various X-chromosomal and autosomal hyperploids are discussed in the light of a model for dosage compensation in Drosophila.

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