Zonal expression of the glucokinase gene in rat liver. Dynamics during the daily feeding rhythm and starvation-refeeding cycle demonstrated by in situ hybridization

1993 ◽  
Vol 99 (2) ◽  
pp. 133-140 ◽  
Author(s):  
F. Eilers ◽  
H. Bartels ◽  
K. Jungermann
Keyword(s):  
In Situ Hybridization ◽  
Rat Liver ◽  
Glucokinase Gene ◽  
Daily Feeding ◽  
Feeding Rhythm

In Situ Hybridization of Ha-RAS during Rat Liver Carcinogenesis

1991 ◽  
pp. 111-118
Author(s):  
V. Préat ◽  
Y. Nizet ◽  
S. Haesen ◽  
M. Roberfroid
Keyword(s):  
In Situ Hybridization ◽  
Rat Liver ◽  
Liver Carcinogenesis

Nonradioactive in Situ Hybridization for Detection of mRNAs of Peroxisomal Enzymes in Rat Liver

1996 ◽  
Vol 804 (1 Peroxisomes) ◽  
pp. 736-738 ◽  
Author(s):  
ARNO SCHAD ◽  
H. DARIUSH FAHIMI ◽  
ALFRED VÖLKL ◽  
EVELINE BAUMGART
Keyword(s):  
In Situ Hybridization ◽  
Rat Liver ◽  
Nonradioactive In Situ Hybridization

scholarly journals Detection of albumin mRNAs in rat liver by in situ hybridization: usefulness of paraffin embedding and comparison of various fixation procedures.

1987 ◽  
Vol 35 (4) ◽  
pp. 453-459 ◽  
Author(s):  
I Tournier ◽  
D Bernuau ◽  
A Poliard ◽  
D Schoevaert ◽  
G Feldmann
Keyword(s):  
Acetic Acid ◽  
In Situ Hybridization ◽  
Rat Liver ◽  
Specific Activity ◽  
Cdna Probe ◽  
Mrna Detection ◽  
Paraffin Embedding ◽  
Ethanol Acetic Acid ◽  
Albumin Mrna

Our aim was to define optimal conditions for efficient and reproducible albumin mRNA detection in rat liver by in situ hybridization. We used an albumin-specific [3H]-labeled cDNA probe with a specific activity of 6-8.10(6) cpm/microgram DNA. In situ hybridization is as efficient on paraffin sections as on cryostat sections for detecting albumin mRNAs. Perfusion fixation with a 4% paraformaldehyde solution results in homogeneous RNA retention within tissue blocks, in contrast with immersion fixation, which yields heterogeneous RNA preservation. Comparison of immersion fixation with three different fixatives (paraformaldehyde, ethanol-acetic acid, and Bouin's fixative) shows that the highest level of hybridization signal is obtained with paraformaldehyde. Ethanol-acetic acid and Bouin's fixative appear less efficient for albumin mRNA detection. Loss of mRNAs within liver tissue blocks over time is largely although not completely prevented by paraffin embedding.

scholarly journals Demonstration by in situ hybridization of the zonal modulation of rat liver cytochrome P-450b and P-450e gene expression after phenobarbital.

1988 ◽  
Vol 82 (2) ◽  
pp. 658-666 ◽  
Author(s):  
E Wojcik ◽  
C Dvorak ◽  
J Chianale ◽  
P G Traber ◽  
D Keren ◽  
...  
Keyword(s):  
Gene Expression ◽  
In Situ Hybridization ◽  
Rat Liver ◽  
Liver Cytochrome
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