Cell-free translation of carnation latent virus RNA and analysis of virus-specific dsRNA

Virus Genes ◽  
1991 ◽  
Vol 5 (2) ◽  
pp. 175-181 ◽  
Author(s):  
Brian M. Meehan ◽  
Peter R. Mills
Virus Genes ◽  
1991 ◽  
Vol 5 (4) ◽  
Author(s):  
GaryD. Foster ◽  
PeterR. Mills

1993 ◽  
Vol 13 (6) ◽  
pp. 3340-3349 ◽  
Author(s):  
X Danthinne ◽  
J Seurinck ◽  
F Meulewaeter ◽  
M Van Montagu ◽  
M Cornelissen

The RNA of satellite tobacco necrosis virus (STNV) is a monocistronic messenger that lacks both a 5' cap structure and a 3' poly(A) tail. We show that in a cell-free translation system derived from wheat germ, STNV RNA lacking the 600-nucleotide trailer is translated an order of magnitude less efficiently than full-size RNA. Deletion analyses positioned the translational enhancer domain (TED) within a conserved hairpin structure immediately downstream from the coat protein cistron. TED enhances translation when fused to a heterologous mRNA, but the level of enhancement depends on the nature of the 5' untranslated sequence and is maximal in combination with the STNV leader. The STNV leader and TED have two regions of complementarity. One of the complementary regions in TED resembles picornavirus box A, which is involved in cap-independent translation but which is located upstream of the coding region.


Cell ◽  
1978 ◽  
Vol 15 (2) ◽  
pp. 607-614 ◽  
Author(s):  
Susan R. Weiss ◽  
Perry B. Hackett ◽  
Hermann Oppermann ◽  
Axel Ullrich ◽  
Leon Levintow ◽  
...  

1987 ◽  
Vol 68 (1) ◽  
pp. 169-180 ◽  
Author(s):  
S. A. Shields ◽  
T. M. A. Wilson

1990 ◽  
Vol 15 (6) ◽  
pp. 937-939 ◽  
Author(s):  
Gary D. Foster ◽  
Brian M. Meehan ◽  
Peter R. Mills

1967 ◽  
Vol 22 (10) ◽  
pp. 1008-1013 ◽  
Author(s):  
C. Wetter

The position (p) of precipitin bands in OUCHTERLONY’S test with tobacco mosaic virus and carnation latent virus depends upon the concentration of agar and electrolyte. This dependence results from the greater diffusional restriction of antigen in comparison to that of antibody. It is suggested that the effect of electrolyte concentration on the antigen in gel is mechanistically the same as the interaction between virus and agar in the sol state. Antisera against seven distantly related viruses of the potato virus S group reacted with carnation latent, potato M, and red clover vein mosaic viruses. This further demonstrates a serological relationship between viruses of the same shape but different normal length.


Author(s):  

Abstract A new distribution map is provided for Carnation latent virus. Flexiviridae: Carlavirus. Hosts: carnation (Dianthus caryophyllus) and sweet william (D. barbatus). Information is given on the geographical distribution in Europe (France, Germany, Lithuania, Spain, Ukraine, and England and Wales, UK), Asia (Himachal Pradesh, India; Honshu, Japan; and Korea Republic), North America (British Columbia, Canada, and Colorado and New York, USA), South America (Argentina and Venezuela) and Oceania (Victoria, Australia).


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