Cell-free translation of avian erythroblastosis virus RNA.

1980 ◽  
Vol 34 (1) ◽  
pp. 280-284 ◽  
Author(s):  
T Pawson ◽  
G S Martin
Keyword(s):  
Virus Rna ◽  
Free Translation ◽  
Avian Erythroblastosis Virus

scholarly journals The 3' untranslated region of satellite tobacco necrosis virus RNA stimulates translation in vitro.

1993 ◽  
Vol 13 (6) ◽  
pp. 3340-3349 ◽  
Author(s):  
X Danthinne ◽  
J Seurinck ◽  
F Meulewaeter ◽  
M Van Montagu ◽  
M Cornelissen
Keyword(s):  
Tobacco Necrosis Virus ◽  
Translation System ◽  
Coding Region ◽  
Necrosis Virus ◽  
Untranslated Sequence ◽  
Virus Rna ◽  
Free Translation ◽  
Order Of Magnitude ◽  
A Cell

The RNA of satellite tobacco necrosis virus (STNV) is a monocistronic messenger that lacks both a 5' cap structure and a 3' poly(A) tail. We show that in a cell-free translation system derived from wheat germ, STNV RNA lacking the 600-nucleotide trailer is translated an order of magnitude less efficiently than full-size RNA. Deletion analyses positioned the translational enhancer domain (TED) within a conserved hairpin structure immediately downstream from the coat protein cistron. TED enhances translation when fused to a heterologous mRNA, but the level of enhancement depends on the nature of the 5' untranslated sequence and is maximal in combination with the STNV leader. The STNV leader and TED have two regions of complementarity. One of the complementary regions in TED resembles picornavirus box A, which is involved in cap-independent translation but which is located upstream of the coding region.

Cell-free translation of avian sarcoma virus RNA: Suppression of the gag termination codon does not augment synthesis of the joint gag/pol Product

Cell ◽  
1978 ◽  
Vol 15 (2) ◽  
pp. 607-614 ◽  
Author(s):  
Susan R. Weiss ◽  
Perry B. Hackett ◽  
Hermann Oppermann ◽  
Axel Ullrich ◽  
Leon Levintow ◽  
...  
Keyword(s):  
Termination Codon ◽  
Avian Sarcoma Virus ◽  
Virus Rna ◽  
Free Translation ◽  
Sarcoma Virus ◽  
Avian Sarcoma

scholarly journals Cell-free Translation of Turnip Mosaic Virus RNA

1987 ◽  
Vol 68 (1) ◽  
pp. 169-180 ◽  
Author(s):  
S. A. Shields ◽  
T. M. A. Wilson
Keyword(s):  
Mosaic Virus ◽  
Turnip Mosaic Virus ◽  
Virus Rna ◽  
Free Translation

scholarly journals Cell-free translation and identification of the replicative form of Nudaurelia beta virus RNA

1991 ◽  
Vol 72 (2) ◽  
pp. 267-273 ◽  
Author(s):  
D. H. du Plessis ◽  
G. Mokhosi ◽  
D. A. Hendry
Keyword(s):  
Replicative Form ◽  
Virus Rna ◽  
Free Translation
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