Effects of evening primrose oil treatment on sciatic nerve blood flow and endoneurial oxygen tension in streptozotocin-diabetic rats

1994 ◽  
Vol 31 (4) ◽  
pp. 220-225 ◽  
Author(s):  
N. E. Cameron ◽  
M. A. Cotter
Keyword(s):  
Blood Flow ◽  
Sciatic Nerve ◽  
Oxygen Tension ◽  
Diabetic Rats ◽  
Evening Primrose Oil ◽  
Nerve Blood Flow ◽  
Evening Primrose ◽  
Streptozotocin Diabetic Rats

scholarly journals Deficient nitric oxide responsible for reduced nerve blood flow in diabetic rats: effects of L-NAME, L-arginine, sodium nitroprusside and evening primrose oil

1996 ◽  
Vol 118 (1) ◽  
pp. 186-190 ◽  
Author(s):  
Nagashige Omawari ◽  
Mark Dewhurst ◽  
Phuong Vo ◽  
Salina Mahmood ◽  
Elizabeth Stevens ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Blood Flow ◽  
Sodium Nitroprusside ◽  
Diabetic Rats ◽  
Evening Primrose Oil ◽  
Nerve Blood Flow ◽  
Evening Primrose

Hypoxic effect of exogenous insulin on normal and diabetic peripheral nerve

1994 ◽  
Vol 266 (6) ◽  
pp. E980-E985 ◽  
Author(s):  
M. Kihara ◽  
P. J. Zollman ◽  
I. L. Smithson ◽  
T. D. Lagerlund ◽  
P. A. Low
Keyword(s):  
Blood Flow ◽  
Peripheral Nerve ◽  
Oxygen Tension ◽  
Glycosylated Hemoglobin ◽  
Diabetic Rats ◽  
Insulin Administration ◽  
Dissociation Curve ◽  
Nerve Blood Flow ◽  
Intravenous Insulin ◽  
Arteriovenous Shunts

Insulin administration can cause or worsen experimental and human diabetic neuropathy ("insulin neuritis"). In this study, we tested the hypothesis that insulin administration impairs tissue oxygenation. We infused insulin under nonhypoglycemic conditions and evaluated its effect on endoneurial oxygen tension, nerve blood flow, and the oxyhemoglobin dissociation curve of peripheral nerve in normal and diabetic rats. Intravenous insulin infusion resulted in a dose-dependent reduction in endoneurial oxygen tension in normal nerves (from 26% at 0.04 U/kg insulin to 55% at 32 U/kg). The nerves of rats with streptozotocin-induced diabetes were resistant, but with control of hyperglycemia this susceptibility to the endoneurial hypoxic effect of insulin returned. The reduction in endoneurial oxygen tension regressed with glycosylated hemoglobin (Y = 53.8-2.7X, where Y = %reduction in endoneurial oxygen tension and X = HbA1; r = 0.87; P = < 0.001). Diabetes or insulin administration resulted in only minimal and physiologically insignificant alterations in the oxygen dissociation curve and 2,3-diphosphoglycerate of sciatic nerve. Instead, insulin administration resulted in a reduction in nerve nutritive blood flow and an increase in arteriovenous shunt flow. When the latter was eliminated by the closure of arteriovenous shunts (infusion of 5-hydroxytryptamine), endoneurial oxygen reverted to normal. These findings indicate a deleterious vasoactive effect of insulin and may explain the development of insulin neuritis.

Altered response in renal blood flow and oxygen tension to contrast media in diabetic rats

2003 ◽  
Vol 44 (3) ◽  
pp. 347-353 ◽  
Author(s):  
F. Palm ◽  
P.-O. Carlsson ◽  
P. Hansell ◽  
O. Hellberg ◽  
A. Nygren ◽  
...  
Keyword(s):  
Blood Flow ◽  
Oxygen Tension ◽  
Blood Glucose Concentration ◽  
Diabetic Control ◽  
Diabetic Rats ◽  
Diabetic Patients ◽  
Medullary Blood Flow ◽  
Streptozotocin Diabetic Rats ◽  
Altered Response

Purpose: To investigate the effect of the contrast medium (CM) iopromide on renal microcirculation and oxygen tension in non-diabetic control and streptozotocin-diabetic Wistar Furth rats. Materials and Methods: Oxygen tension was measured with Clark-type microelectrodes and blood flow with laser-Doppler flow probes. In order to differentiate between an acutely increased blood glucose concentration and a long-term diabetic state, some of the non-diabetic control rats were intravenously infused with glucose. Results: CM decreased the medullary oxygen tension in control (non-diabetic normoglycemic) rats (∼35%) but not in diabetic rats. Medullary blood flow in control rats increased after CM administration and remained elevated, while it was unchanged in the diabetic rats. In response to CM, glucose-infused control rats responded similarly to control animals in medullary oxygen tension, but similarly to diabetic rats in medullary blood flow. Contrary to in control rats, medullary oxygen tension was unchanged in diabetic animals after CM administration. Conclusion: Streptozotocin-diabetic rats have an altered response to intravenous injection of the CM iopromide compared to non-diabetic rats. The unaltered medullary oxygen tension, seen in the diabetic group after injection of CM, suggests that non-hemodynamic mechanisms are responsible for the increased frequency of renal failure commonly seen among diabetic patients.

Increased sciatic nerve blood flow in diabetic rats: assessment by "molecular" vs. particulate microspheres

1997 ◽  
Vol 273 (1) ◽  
pp. E164-E173 ◽  
Author(s):  
K. Chang ◽  
Y. Ido ◽  
W. LeJeune ◽  
J. R. Williamson ◽  
R. G. Tilton
Keyword(s):  
Blood Flow ◽  
Sciatic Nerve ◽  
Reference Sample ◽  
Streptozotocin Diabetes ◽  
Diabetic Rats ◽  
Nerve Blood Flow ◽  
Doppler Flowmetry ◽  
Blood Flows ◽  
Microsphere Method ◽  
Onset Of Diabetes

Sciatic nerve blood flow in diabetic rats in typically increased or unchanged when assessed by the reference sample microsphere method in our laboratory. In contrast, blood flow is generally reported to be decreased approximately 50% when assessed with laser Doppler flowmetry or hydrogen clearance polarography. To address concerns that increased blood flow observed with microspheres might be anomalous because of their particulate nature and/or because insufficient numbers of microspheres are captured in the nerve, a plasma-soluble "molecular microsphere" ([3H]desmethylimipramine, mol wt = 266) and 11.3-micron 153Gd-labeled microspheres were injected sequentially to assess blood flow in rats with streptozotocin diabetes of 2-4 wk duration. Nerve blood flows in diabetic rats were increased 1.5- to 2-fold (vs. control rats) with both tracers; these increases were prevented by tolrestat, an inhibitor of aldose reductase. These observations indicate that blood flow in sciatic nerve (like that in retina and kidney) is increased early after the onset of diabetes and is 1) demonstrable with a plasma-soluble tracer as well as with particulate microspheres and 2) linked to increased metabolism of glucose via the sorbitol pathway.

A disturbed macrocirculatory supply as a determinant for a reduced sciatic nerve blood flow in diabetic rats

1996 ◽  
Vol 318 (1) ◽  
pp. 73-80 ◽  
Author(s):  
Theo Van Buren ◽  
Arnoud C Kappelle ◽  
Carina M Kasbergen ◽  
Willem H Gispen ◽  
Dick J De Wildt
Keyword(s):  
Blood Flow ◽  
Sciatic Nerve ◽  
Diabetic Rats ◽  
Nerve Blood Flow

Presynaptic deficit of sympathetic nerves: a cause for disturbed sciatic nerve blood flow responsiveness in diabetic rats

1996 ◽  
Vol 296 (3) ◽  
pp. 277-283 ◽  
Author(s):  
Theo Van Buren ◽  
Carina M. Kasbergen ◽  
Willem H. Gispen ◽  
Dick J. De Wildt
Keyword(s):  
Blood Flow ◽  
Sciatic Nerve ◽  
Sympathetic Nerves ◽  
Diabetic Rats ◽  
Nerve Blood Flow

Diabetes impairs sciatic nerve hyperemia induced by surgical trauma: implications for diabetic neuropathy

1997 ◽  
Vol 273 (1) ◽  
pp. E174-E184 ◽  
Author(s):  
Y. Ido ◽  
K. Chang ◽  
W. LeJeune ◽  
R. G. Tilton ◽  
W. W. Monafo ◽  
...  
Keyword(s):  
Blood Flow ◽  
Sciatic Nerve ◽  
Diabetic Rats ◽  
Surgical Trauma ◽  
Nerve Blood Flow ◽  
Doppler Flowmetry ◽  
Blood Flow Response ◽  
Flow Response ◽  
Surgical Exposure ◽  
Minimal Trauma

The most widely used methods to assess nerve blood flow in diabetics rats are hydrogen clearance polarography and laser Doppler flowmetry, techniques requiring surgical exposure of the nerve. In these experiments, we examined the hypothesis that the trauma of surgical exposure introduces an important and hitherto largely unrecognized variable that could account for discordant reports on nerve blood flow changes induced by diabetes. We used the noninvasive (for sciatic nerve) reference sample microsphere method to quantify sciatic nerve blood flow in unexposed va. surgically exposed nerves in rats with streptozotocin-induced diabetes (at different temperatures and after curarization) and in unexposed vs. surgically exposed nerves in galactose-fed rats. Baseline resting blood flow in unexposed nerves in both animal models of diabetes was either normal or increased (but was decreased in diabetic rats given d-tubocurarine). Furthermore, the normal brisk hyperemic nerve blood flow response to the minimal trauma associated with surgical exposure of the nerve was markedly impaired in diabetic and in galactose-fed rats. Normalization of the blood flow response to trauma in galactose-fed rats by an aldose reductase inhibitor suggests that the impairment is linked to increased polyol pathway metabolism. These findings 1) confirm our previous findings that sciatic nerve blood flow in diabetic rats is increased or unchanged in unexposed nerves, while also confirming reports that in surgically exposed nerves blood flow is higher in control than in diabetic rats, and 2) indicate that blood flows in surgically exposed nerves are largely a measure of vascular responses to injury rather than (patho)physiological blood flow in undisturbed nerves.

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