The genome of the Mediterranean fruitflyceratitis capitata: Localization of molecular markers by in situ hybridization to salivary gland polytene chromosomes

Nine specific DNA probes (genomic or cDNA) from Ceratitis capitata have been mapped by in situ hybridization to the salivary gland polytene chromosomes of the olive fruit fly Bactrocera oleae, a major agricultural pest, thus establishing molecular markers for the 5 autosomal chromosomes. Taking into account the present results, as well as previous data obtained mainly by in situ hybridizations, chromosomal homologies among B. oleae, C. capitata and B. tryoni are established. Data show extensive linkage group conservation among the 3 taxa of the economically important and globally distributed family, the Tephritidae.Key words: Bactrocera oleae, Tephritidae, salivary gland, polytene chromosomes, in situ hybridization, mapping.

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UNDER-REPLICATION OF RIBOSOMAL CISTRONS IN POLYTENE CHROMOSOMES OF RHYNCHOSCIARA

The Journal of Cell Biology ◽

10.1083/jcb.62.1.215 ◽

1974 ◽

Vol 62 (1) ◽

pp. 215-222 ◽

Cited By ~ 16

Author(s):

A. G. Gambarini ◽

F. J. S. Lara

Keyword(s):

Salivary Gland ◽

In Situ Hybridization ◽

Xenopus Laevis ◽

Related Species ◽

Polytene Chromosomes ◽

Chromosome X ◽

Heterologous Hybridization ◽

Ribosomal Cistrons ◽

Rhynchosciara Americana

DNA preparations obtained from several tissues of Rhynchosciara americana and two related species, R. milleri and R. papaveroi, were hybridized to R. americana rRNA. The percentage of hybridization was found to be higher in tissues with low polyteny than in tissues with high polyteny, suggesting a relationship between the amount of rDNA and the tissue polyteny. This could be explained by under-replication of ribosomal cistrons in polytene cells, such as those from the salivary gland. Only slight tissue-dependent changes in the percentages of hybridization can be observed in heterologous hybridization using Xenopus laevis rRNA. The possibility that these experiments could not detect differences in the amount of ribosomal cistrons among tissues is discussed. The female:male ratio for the percentages of hybridization in the salivary gland of R. americana agrees with the results obtained by in situ hybridization experiments (16, 17) which have shown that the rRNA cistrons are distributed among chromosomes other than chromosome X.

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Molecular characterization and chromosomal localization of female-specific genes from the Mediterranean fruit fly Ceratitis capitata (Diptera: Tephritidae)

Genome ◽

10.1139/g04-080 ◽

2005 ◽

Vol 48 (1) ◽

pp. 139-144 ◽

Cited By ~ 1

Author(s):

Silvia Ciolfi ◽

Tiziana de Filippis ◽

Cristina Torti ◽

Anna R Malacrida ◽

Romano Dallai

Keyword(s):

In Situ Hybridization ◽

Molecular Characterization ◽

Ceratitis Capitata ◽

Polytene Chromosomes ◽

Fruit Fly ◽

Accessory Gland ◽

Mediterranean Fruit Fly ◽

The Mediterranean ◽

Female Specific

We report here the molecular characterization of the female-specific FST (female-specific transcript) genes from the Mediterranean fruit fly (medfly) Ceratitis capitata. A genomic clone was isolated, containing a sequence coding for FST. Nucleotide analysis of the clone showed that the gene contains a putative unique intron located in the region encoding the signal peptide. Southern blotting and in situ hybridization analysis on polytene chromosomes suggested the presence of additional genes similar to FST in the genome of the medfly. A novel cDNA clone was isolated from an accessory gland cDNA library, encoding a product that shares 98% identity with the hypothetical translational product of the previously isolated FST cDNA. The novel cDNA was therefore named FST2. The analysis of mitotic and polytene chromosomes by in situ hybridization showed that FST genes map on the left arm of the 4th chromosome of C. capitata.Key words: FST, female-specific genes, C. capitata, medfly, FISH.

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Giant Cell Carcinosarcoma of the Parotid Gland With a PLAG 1 Translocation in Association With a Pleomorphic Adenoma With HMGA2 Translocation

American Journal of Clinical Pathology ◽

10.1093/ajcp/aqaa104 ◽

2020 ◽

Vol 154 (6) ◽

pp. 811-815

Author(s):

Levon Katsakhyan ◽

Virginia A LiVolsi ◽

Ara A Chalian ◽

Paul J Zhang

Keyword(s):

Salivary Gland ◽

In Situ Hybridization ◽

Parotid Gland ◽

Giant Cell ◽

Pleomorphic Adenoma ◽

De Novo ◽

Sarcomatous Component ◽

Gland Tumor ◽

Parotid Gland Tumor

Abstract Objectives Carcinosarcomas of the salivary gland are rare neoplasms and have been described arising de novo or in association with pleomorphic adenoma (PA). PLAG1 and HMGA2 translocations are known to occur in PAs and carcinomas ex PA but are mutually exclusive. Methods We report a case of a carcinosarcoma in the parotid gland of a 77-year-old man with unusual anaplastic sarcomatoid giant cell morphology. Results Microscopically, a small separate PA was found adjacent to the carcinosarcoma. By conventional notion, the PA and carcinosarcoma would be considered related, as carcinosarcomas are well known to arise from PAs (carcinosarcoma ex PA). However, fluorescence in situ hybridization (FISH) assay demonstrated PLAG1 translocation in the carcinosarcoma and HMGA2 translocation in the separate PA. Conclusions These findings support that the carcinosarcoma likely originated from another PA with a PLAG1 translocation or de novo but not from the coexisting PA harboring a different translocation. To our knowledge, the case is the first to demonstrate PLAG1 translocation by FISH in a sarcomatous component of any parotid gland tumor, which may help better classify these tumors. In addition, multiple PAs are commonly found in the salivary gland, and to our knowledge, our case is the first to demonstrate that the same parotid gland can host PAs and PA-related tumors with different translocations.

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Detection of rRNA and phaseolin genes on polytene chromosomes of Phaseolus coccineus by fluorescence in situ hybridization after pepsin pretreatment

Genome ◽

10.1139/g94-144 ◽

1994 ◽

Vol 37 (6) ◽

pp. 1018-1021 ◽

Cited By ~ 12

Author(s):

M. Nenno ◽

K. Schumann ◽

W. Nagl

Keyword(s):

In Situ Hybridization ◽

Fluorescence In Situ Hybridization ◽

Ribosomal Rna ◽

Storage Protein ◽

Seed Storage Protein ◽

Polytene Chromosomes ◽

Seed Storage ◽

Phaseolus Coccineus ◽

Rna Genes

This is the first report of fluorescence in situ hybridization (FISH) on plant polytene chromosomes. Different protease pretreatments have been tested to improve fluorescence in situ hybridization FISH on polytene chromosomes of a plant, Phaseolus coccineus, with the aim to enable the detection of low-copy genes. The structural preservation of the chromosomes and the distinctness of the FISH signals were comparatively analysed with a probe for the ribosomal RNA genes after digestion with pepsin and trypsin. The pepsin pretreatment resulted in a general loosening of chromatin with good conservation of chromosome morphology and an increased number and density of signal points. The six nucleolus organizers exhibited significant differences in condensation. The pretreatment with pepsin enabled the detection of the low-copy genes encoding the seed storage protein phaseolin.Key words: plant, Leguminosae, ribosomal RNA genes, seed storage protein genes, protease.

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