Investigations on paper chromatographic separation of serum lipoproteins

1958 ◽  
Vol 45 (10) ◽  
pp. 241-241 ◽  
Author(s):  
Č. Michalec ◽  
M. Štastný ◽  
E. Nováková
Keyword(s):  
Chromatographic Separation ◽  
Serum Lipoproteins ◽  
Paper Chromatographic Separation

scholarly journals Erratum

1959 ◽  
Vol 5 (6) ◽  
pp. 574-574
Keyword(s):  
Human Serum ◽  
Chromatographic Separation ◽  
Serum Lipoproteins ◽  
Paper Chromatographic Separation

Abstract An error appeared in Abstract No. 21, "The Paper Chromatographic Separation of Human Serum Lipoproteins," by McDonald, Banaszak and Kissane, Clin. Chem. 5, 270, (1959). In the sixth line of the abstract, the phrase "13.3 Gm. sodium veronal" should read "10.3 Gm. sodium veronal."

The Metabolism of Vernolate-14C in Soybean Seedlings

Weed Science ◽  
1968 ◽  
Vol 16 (3) ◽  
pp. 290-292 ◽  
Author(s):  
John B. Bourke ◽  
S. C. Fang
Keyword(s):  
Glycine Max ◽  
Relative Abundance ◽  
Chromatographic Separation ◽  
Time Course ◽  
Soybean Seedlings ◽  
Time Course Study ◽  
Ethanol Extracts ◽  
Cellular Incorporation ◽  
Paper Chromatographic Separation

The metabolism of S-propyl-1-14C dipropylthiocarbamate (vernolate) was studied in soybean (Glycine max (L.) Merr., var. Lee) seedlings. The degradation of vernlate-14C to 14CO2 was dependent on the age of seedlings. Pretreatment with non-labeled vernolate resulted in a reduction of catabolic oxidation of vernolate-14C to 14CO2, a reduction of cellular incorporation, and a corresponding increase in ethanol soluble metabolites. Paper chromatographic separation of ethanol extracts revealed the presence of two major metabolites and two minor metabolites. The relative abundance of these metabolites was dependent on the age of seedlings and the time of exposure. Results of the time course study suggested the conversion of metabolite 3 to metabolite 4.

scholarly journals ON THE RELATION OF WHITE BLOOD CELLS AND PLATELETS TO VENOM HEMOLYSIS

1956 ◽  
Vol 104 (4) ◽  
pp. 517-523 ◽  
Author(s):  
Joseph C. Turner ◽  
Keyword(s):  
Guinea Pig ◽  
Hemolytic Activity ◽  
Chromatographic Separation ◽  
Blood Cells ◽  
Direct Action ◽  
White Blood Cells ◽  
Substantial Reduction ◽  
Red Cells ◽  
Phospholipase Activity ◽  
Paper Chromatographic Separation

Removal of the white cells and platelets from suspensions of red cells usually produces substantial reduction in the hemolytic activity of venoms. Guinea pig red cells constitute a notable exception and may be lysed by a direct action of venom. White blood cells and platelets appear to contribute to hemolysis by serving as sources of phosphatides for the formation of lysophosphatides. No correlation could be found between phospholipase activity and direct hemolytic activity of venoms. A recently described method (8) of paper chromatographic separation of phospholipides has been used successfully in part of the work.

scholarly journals A Paper Chromatographic Separation of Gibberellic Acid and Gibberellin A.

1958 ◽  
Vol 33 (1) ◽  
pp. 45-46 ◽  
Author(s):  
Harold L. Bird ◽  
Charles T. Pugh
Keyword(s):  
Gibberellic Acid ◽  
Chromatographic Separation ◽  
Paper Chromatographic Separation

Identification of Grape Varieties

1965 ◽  
Vol 48 (3) ◽  
pp. 525-530
Author(s):  
A C Rice
Keyword(s):  
Chromatographic Separation ◽  
Quantitative Data ◽  
Spectral Characteristics ◽  
Grape Juice ◽  
Concord Grape ◽  
Anthocyanin Pigments ◽  
Grape Varieties ◽  
Paper Chromatographic Separation ◽  
Time Of Harvest

Abstract The spectrophotometric characteristics of Concord grape juice have been found to vary greatly with season, growing area, and time of harvest. As a result, the spectral characteristics of the juice are not a suitable basis for a method to detect adulteration of the juice. The paper chromatographic separation of the anthocyanin pigments in Concord grape juice has been used successfully to obtain pure components, of which 10 have been identified. Quantitative data can now be obtained from the paper chromatograms by a Beckman Anatrol. A measure of the ratios of several of the anthocyanin pigments present in Concord juice is promising as a method of detecting adulteration.

scholarly journals A colorimetric micro method for the determination of formyl groups

1969 ◽  
Vol 111 (4) ◽  
pp. 401-406 ◽  
Author(s):  
S. Usha Lakshmi ◽  
L. K. Ramachandran
Keyword(s):  
Amino Acids ◽  
Molecular Weight ◽  
Chromatographic Separation ◽  
Quantitative Method ◽  
Standard Procedure ◽  
Formyl Group ◽  
Alumina Column ◽  
Purple Colour ◽  
Paper Chromatographic Separation

The characteristic purple colour formed by N-formyl-N′-2,4-dinitrophenyl-hydrazine in the presence of piperidine and acetone was made the basis of a new quantitative method for the determination of formyl groups. Samples containing N-formyl groups (up to 0·4μmole) are hydrazinolysed at 97–98° for 1hr. and are dinitrophenylated after the removal of excess of hydrazine. Interference from 2,4-dinitrophenylhydrazine is eliminated by subjecting the dinitrophenylated samples to chromatography on an alumina column. Interference arising from the formation of N-acetyl-N′-2,4-dinitrophenylhydrazine, when determining formyl groups in samples containing acetyl, can be avoided by a paper-chromatographic separation before analysis. A standard procedure is described. The method gives satisfactory results when applied to N-formyl-amino acids. Gramicidin, when analysed by this method, was found to contain 0·89 mole of formyl group/mole for a molecular weight of 1880. The method indicated the absence of formyl groups from lysozyme, a protein known not to contain such groups. Generally, the analytical values obtained by the method are within 100±4% of theory.

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