Influence of Contact Time on Loading 99Mo(n,γ) with Alumina Column in 99Mo / 99mTc Generator

<p>Alumina is usually used for ⁹⁹Mo absorbers obtained from ²³⁵U fission product because the use of ²³⁵U is getting limited, and as an alternative ⁹⁹Mo obtained from natural MoO₃ irradiation was used. The problem is that ⁹⁹Mo produced was not carrier-free as ⁹⁹Mo from ²³⁵U fission product because not all MoO₃ is activated to ⁹⁹Mo, then it is necessary to know the effect of contact time when loading ⁹⁹Mo into the alumina column. To find out the effect of contact time, the experiment was carried out using ⁹⁹Mo pH 4, acid alumina column, contact time was varied between 1 to 60 minutes with interval of 15 minutes, and the column was eluted using 2 kinds of eluent namely 0.9% NaCl and MEK. The elution of ⁹⁹Mo/^99mTc generator is performed 3 times, days 1, 2 and 3. The results showed that the performance of alumina in absorbing ⁹⁹Mo influenced by time. The optimal contact time is 15 minutes for 0.9% NaCl solution and 45 minutes for MEK. The amount of ⁹⁹Mo breakthrough (µCi⁹⁹Mo/mCi^99mTc) from the alumina column increases with elution time both using 0.9% NaCl or MEK. The ^99mTc eluate obtained from this study does not meet standard requirements. The use of a tandem column is needed to prevent ⁹⁹Mo breakthrough on the ⁹⁹Mo/^99mTc generator to get the ^99mTc which meet standard requirement.</p>

PENGARUH REGENERASI KOLOM ALUMINA ASAM TERHADAP RECOVERY DAN KUALITAS 99mTc HASIL EKSTRAKSI PELARUT MEK DARI 99Mo HASIL AKTIVASI NEUTRON

PENGARUH REGENERASI KOLOM ALUMINA ASAM TERHADAP RECOVERY DAN KUALITAS 99mTc HASIL EKSTRAKSI PELARUT MEK DARI 99Mo HASIL AKTIVASI NEUTRON. Melalui kerjasama antara PTRR-BATAN, Chiyoda dan JAEA Jepang telah dilakukan pemurnian 99mTc dari 99Mo hasil aktivasi neutron dengan menggunakan metode kromatografi kolom alumina asam terhadap hasil ekstraksi MEK (Metil Etil Keton). Pemurnian 99mTc dengan metode kolom alumina asam hanya dapat digunakan satu kali dan pemurnian berikutnya harus diganti dengan kolom baru. Hal ini dinilai kurang praktis dan juga memerlukan biaya yang mahal. Dalam penelitian ini dicoba penggunaan kolom alumina asam untuk pemurnian 99mTc lebih dari satu kali dengan melakukan proses regenerasi dengan cara melewatkan larutan HNO3 0,1N setiap kali proses pemurnian selesai. Penelitian ini bertujuan untuk mendapatkan larutan 99mTc yang dapat digunakan untuk penandaan kit radiofarmaka. Parameter yang diamati dalam penelitian ini adalah recovery, profil elusi, pH, kemurnian radiokimia dan kemurnian radionuklida (lolosan 99Mo). Hasil penelitian yang dilakukan selama 5 hari telah diperoleh pH ~5, recovery > 60 %, kemurnian radiokimia > 95 % dan lolosan 99Mo tidak terdeteksi. Dari hasil perlakuan terhadap kolom alumina asam dengan larutan HNO3 0,1 N disimpulkan bahwa kolom alumina asam tidak perlu diganti setiap hari.Kata kunci: 99mTc, 99Mo, MEK, kolom alumina asam, kemurnian radiokimia. Purification of 99mTc from 99Mo activation using acidic alumina column chromatography system from MEK (Methyl Ethyl Keton) extraction has been carried out through cooperation between PTRR - BATAN, Chiyoda and JAEA Japan. This method has a limitation that acidic alumina column for purification of 99mTc can be used only once, for the next purification acidic alumina column should be replaced with new column, so it is less practical and also requires high cost. This study aims to obtain a 99mTc solution can be use for labelling of a radiopharmaceutical kit. In this study, the used of acidic alumina column for 99mTc purification was tried more than once by regeneration using 0.1N HNO3 solution after purification process is completed. Parameters observed in this study are the percent recovery, elution profile, pH, radiochemical purity and radionuclida purity. The results of observational studies conducted over 5 days has been obtained pH ~ 5,% recovery > 60%, radiochemical purity of > 95% and 99Mo leakage not detected. The treatment of acidic alumina column with 0,1 N HNO3 solution concluded that acidic alumina column does not need to be replaced every day.Keywords: 99mTc, 99Mo, MEK, acidic alumina column, radiochemical purity.