Electrical and mechanical stimulation of hair cells in the mudpuppy

1975 ◽  
Vol 102 (1) ◽  
pp. 13-26 ◽  
Author(s):  
Olav Sand ◽  
Seiji Ozawa ◽  
Susumu Hagiwara
1994 ◽  
Vol 427 (1-2) ◽  
pp. 162-168 ◽  
Author(s):  
Christian Chabbert ◽  
Gwenaelle Geleoc ◽  
Jacques Lehouelleur ◽  
Alain Sans

eLife ◽  
2016 ◽  
Vol 5 ◽  
Author(s):  
Aakash Basu ◽  
Samuel Lagier ◽  
Maria Vologodskaia ◽  
Brian A Fabella ◽  
AJ Hudspeth

Mechanoelectrical transduction by hair cells commences with hair-bundle deflection, which is postulated to tense filamentous tip links connected to transduction channels. Because direct mechanical stimulation of tip links has not been experimentally possible, this hypothesis has not been tested. We have engineered DNA tethers that link superparamagnetic beads to tip links and exert mechanical forces on the links when exposed to a magnetic-field gradient. By pulling directly on tip links of the bullfrog's sacculus we have evoked transduction currents from hair cells, confirming the hypothesis that tension in the tip links opens transduction channels. This demonstration of direct mechanical access to tip links additionally lays a foundation for experiments probing the mechanics of individual channels.


2016 ◽  
Author(s):  
Aakash Basu ◽  
Samuel Lagier ◽  
Maria Vologodskaia ◽  
Brian A Fabella ◽  
AJ Hudspeth

1988 ◽  
Vol 35 (2-3) ◽  
pp. 201-207 ◽  
Author(s):  
Dennis M Freeman ◽  
Thomas F Weiss

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