Routes of tetanus toxin entrance into the central nervous system and some problems in the pathogenesis of experimental tetanus

1961 ◽  
Vol 51 (3) ◽  
pp. 298-304 ◽  
Author(s):  
G. N. Kryzhanovskii ◽  
L. A. Pevnitskii ◽  
V. N. Grafova ◽  
A. A. Polgar
Keyword(s):  
Central Nervous System ◽  
Nervous System ◽  
Tetanus Toxin ◽  
Experimental Tetanus ◽  
The Central Nervous System

Routes of entrance of tetanus toxin into the central nervous system and certain questions in the pathogenesis of experimental tetanus

1962 ◽  
Vol 52 (6) ◽  
pp. 1370-1377 ◽  
Author(s):  
G. N. Kryzhanovskii ◽  
L. A. Pevnitskii ◽  
V. N. Grafova ◽  
A. A. Polgar
Keyword(s):  
Central Nervous System ◽  
Nervous System ◽  
Tetanus Toxin ◽  
Experimental Tetanus ◽  
The Central Nervous System

Route of uptake of tetanus toxin into the central nervous system and some problems of the pathogenesis of experimental tetanus

1962 ◽  
Vol 2 (5) ◽  
pp. 534
Author(s):  
G. N. Kryzhanovsky ◽  
L. A. Pevnitsky ◽  
V. N. Grafova ◽  
A. A. Polgar
Keyword(s):  
Central Nervous System ◽  
Nervous System ◽  
Tetanus Toxin ◽  
Experimental Tetanus ◽  
The Central Nervous System

Routes of tetanus toxin entrance into the central nervous system and some problems of experimental tetanus pathogenesis

1962 ◽  
Vol 52 (5) ◽  
pp. 1256-1262 ◽  
Author(s):  
G. N. Kryzhanovskii ◽  
L. A. Pevnitskii ◽  
V. N. Grafova ◽  
A. A. Polgar
Keyword(s):  
Central Nervous System ◽  
Nervous System ◽  
Tetanus Toxin ◽  
Experimental Tetanus ◽  
The Central Nervous System

Cholinesterase activity of the central nervous system and muscles in the course of experimental tetanus

1966 ◽  
Vol 61 (5) ◽  
pp. 503-505
Author(s):  
L. V. Donskaya ◽  
N. E. Zhebrovskaya ◽  
G. N. Tsybulyak
Keyword(s):  
Central Nervous System ◽  
Nervous System ◽  
Cholinesterase Activity ◽  
Experimental Tetanus ◽  
The Central Nervous System

scholarly journals Retrograde axonal transport of an exogenous enzyme covalently linked to B-IIb fragment of tetanus toxin

1990 ◽  
Vol 271 (1) ◽  
pp. 87-91 ◽  
Author(s):  
P Beaude ◽  
A Delacour ◽  
B Bizzini ◽  
D Domuado ◽  
M H Remy
Keyword(s):  
Central Nervous System ◽  
Nervous System ◽  
Glucose Oxidase ◽  
Axonal Transport ◽  
Blood Brain Barrier ◽  
Tetanus Toxin ◽  
Storage Disease ◽  
Retrograde Axonal Transport ◽  
Brain Barrier ◽  
The Central Nervous System

Attempt to replace enzymes in a number of fatal lysosomal storage disease involving the central nervous system have as yet been unsuccessful owing to the impermeability of the blood/brain barrier to macromolecules. In order to treat storage disease due to enzyme deficiencies, we investigated the feasibility of transporting an enzyme into the central nervous system without crossing the blood/brain barrier. Using the B-IIb fragment of tetanus toxin (because it is involved in recognition by the nerve-cell endings), retrograde axonal transport toward the spinal cord and trans-synaptic movement, and glucose oxidase as a marker, we demonstrated that a non-toxic enzyme-vector conjugate was taken up by axon terminals. After injection into the gastrocnemius muscle, the B-IIb-glucose oxidase conjugate was detected, both histologically and electrochemically, distally to a ligature on the sciatic nerve. Thus the B-IIb fragment could serve as a vector for glucose oxidase transport into the central nervous system. It was also verified that the transported enzyme retained its activity. Transport of this 150 kDa molecule by fragment B-IIb of tetanus toxin suggests that other enzymes of a lesser molecular mass may also be transported.

Sign in / Sign up

Export Citation Format

Share Document