Characterization and comparison of merozoite antigens of differentBabesia canis isolates by serological and immunological investigations

1995 ◽  
Vol 81 (8) ◽  
pp. 638-642 ◽  
Author(s):  
Susann Hauschild ◽  
P. Shayan ◽  
E. Schein
Keyword(s):  
Merozoite Antigens

scholarly journals Measuring antibody avidity to Plasmodium falciparum merozoite antigens using a multiplex immunoassay approach

2020 ◽  
Vol 19 (1) ◽  
Author(s):  
Diane Wallace Taylor ◽  
Naveen Bobbili ◽  
Alex Kayatani ◽  
Samuel Tassi Yunga ◽  
Winifrida Kidima ◽  
...  
Keyword(s):  
Plasmodium Falciparum ◽  
Antibody Avidity ◽  
Multiplex Immunoassay ◽  
Falciparum Merozoite ◽  
Merozoite Antigens

scholarly journals Enhancing Blockade of Plasmodium falciparum Erythrocyte Invasion: Assessing Combinations of Antibodies against PfRH5 and Other Merozoite Antigens

2012 ◽  
Vol 8 (11) ◽  
pp. e1002991 ◽  
Author(s):  
Andrew R. Williams ◽  
Alexander D. Douglas ◽  
Kazutoyo Miura ◽  
Joseph J. Illingworth ◽  
Prateek Choudhary ◽  
...  
Keyword(s):  
Plasmodium Falciparum ◽  
Erythrocyte Invasion ◽  
Merozoite Antigens

Systematic analysis of natural antibody responses to P. falciparum merozoite antigens by protein arrays

2013 ◽  
Vol 78 ◽  
pp. 148-158 ◽  
Author(s):  
Yan-Ting Fan ◽  
Yue Wang ◽  
Chuan Ju ◽  
Ting Zhang ◽  
Bin Xu ◽  
...  
Keyword(s):  
Antibody Responses ◽  
Natural Antibody ◽  
Protein Arrays ◽  
Systematic Analysis ◽  
Falciparum Merozoite ◽  
Merozoite Antigens

scholarly journals Complement Activation by Merozoite Antigens of Plasmodium falciparum

PLoS ONE ◽  
2014 ◽  
Vol 9 (8) ◽  
pp. e105093 ◽  
Author(s):  
Jackson C. Korir ◽  
Nancy K. Nyakoe ◽  
George Awinda ◽  
John N. Waitumbi
Keyword(s):  
Plasmodium Falciparum ◽  
Complement Activation ◽  
Merozoite Antigens

scholarly journals Immunoglobulin G Subclass-Specific Responses against Plasmodium falciparum Merozoite Antigens Are Associated with Control of Parasitemia and Protection from Symptomatic Illness

2009 ◽  
Vol 77 (3) ◽  
pp. 1165-1174 ◽  
Author(s):  
Danielle I. Stanisic ◽  
Jack S. Richards ◽  
Fiona J. McCallum ◽  
Pascal Michon ◽  
Christopher L. King ◽  
...  
Keyword(s):  
Plasmodium Falciparum ◽  
Immunoglobulin G ◽  
Vaccine Development ◽  
Surface Protein ◽  
Merozoite Surface Protein ◽  
Igg Subclass ◽  
Apical Membrane Antigen 1 ◽  
Falciparum Merozoite ◽  
Merozoite Antigens

ABSTRACT Substantial evidence indicates that antibodies to Plasmodium falciparum merozoite antigens play a role in protection from malaria, although the precise targets and mechanisms mediating immunity remain unclear. Different malaria antigens induce distinct immunoglobulin G (IgG) subclass responses, but the importance of different responses in protective immunity from malaria is not known and the factors determining subclass responses in vivo are poorly understood. We examined IgG and IgG subclass responses to the merozoite antigens MSP1-19 (the 19-kDa C-terminal region of merozoite surface protein 1), MSP2 (merozoite surface protein 2), and AMA-1 (apical membrane antigen 1), including different polymorphic variants of these antigens, in a longitudinal cohort of children in Papua New Guinea. IgG1 and IgG3 were the predominant subclasses of antibodies to each antigen, and all antibody responses increased in association with age and exposure without evidence of increasing polarization toward one subclass. The profiles of IgG subclasses differed somewhat for different alleles of MSP2 but not for different variants of AMA-1. Individuals did not appear to have a propensity to make a specific subclass response irrespective of the antigen. Instead, data suggest that subclass responses to each antigen are generated independently among individuals and that antigen properties, rather than host factors, are the major determinants of IgG subclass responses. High levels of AMA-1-specific IgG3 and MSP1-19-specific IgG1 were strongly predictive of a reduced risk of symptomatic malaria and high-density P. falciparum infections. However, no antibody response was significantly associated with protection from parasitization per se. Our findings have major implications for understanding human immunity and for malaria vaccine development and evaluation.

scholarly journals Analysis of Antibodies to Newly Described Plasmodium falciparum Merozoite Antigens Supports MSPDBL2 as a Predicted Target of Naturally Acquired Immunity

2013 ◽  
Vol 81 (10) ◽  
pp. 3835-3842 ◽  
Author(s):  
Kevin K. A. Tetteh ◽  
Faith H. A. Osier ◽  
Ali Salanti ◽  
Gathoni Kamuyu ◽  
Laura Drought ◽  
...  
Keyword(s):  
Plasmodium Falciparum ◽  
Risk Ratio ◽  
Statistical Significance ◽  
Clinical Malaria ◽  
Content Type ◽  
Type Antigen ◽  
Falciparum Merozoite ◽  
Merozoite Antigens ◽  
Allelic Type

ABSTRACTProspective studies continue to identify malaria parasite genes with particular patterns of polymorphism which indicate they may be under immune selection, and the encoded proteins require investigation. Sixteen new recombinant protein reagents were designed to characterize three such polymorphic proteins expressed inPlasmodium falciparumschizonts and merozoites: MSPDBL1 (also termed MSP3.4) and MSPDBL2 (MSP3.8), which possess Duffy binding-like (DBL) domains, and SURFIN4.2, encoded by a member of the surface-associated interspersed (surf) multigene family. After testing the antigenicities of these reagents by murine immunization and parasite immunofluorescence, we analyzed naturally acquired antibody responses to the antigens in two cohorts in coastal Kenya in which the parasite was endemic (Chonyi [n= 497] and Ngerenya [n= 461]). As expected, the prevalence and levels of serum antibodies increased with age. We then investigated correlations with subsequent risk of clinical malaria among children <11 years of age during 6 months follow-up surveillance. Antibodies to the polymorphic central region of MSPDBL2 were associated with reduced risk of malaria in both cohorts, with statistical significance remaining for the 3D7 allelic type after adjustment for individuals' ages in years and antibody reactivity to whole-schizont extract (Chonyi, risk ratio, 0.51, and 95% confidence interval [CI], 0.28 to 0.93; Ngerenya, risk ratio, 0.38, and 95% CI, 0.18 to 0.82). For the MSPDBL1 Palo Alto allelic-type antigen, there was a protective association in one cohort (Ngerenya, risk ratio, 0.53, and 95% CI, 0.32 to 0.89), whereas the other antigens showed no protective associations after adjustment. These findings support the prediction that antibodies to the polymorphic region of MSPDBL2 contribute to protective immunity.

scholarly journals Association of Antibodies to VAR2CSA and Merozoite Antigens with Pregnancy Outcomes in Women Living in Yaoundé, Cameroon

2018 ◽  
Vol 86 (9) ◽  
Author(s):  
Yukie M. Lloyd ◽  
Rui Fang ◽  
Naveen Bobbili ◽  
Koko Vanda ◽  
Elise Ngati ◽  
...  
Keyword(s):  
Pregnant Women ◽  
Pregnancy Outcomes ◽  
Odds Ratio ◽  
Placental Malaria ◽  
Multiple Comparisons ◽  
Plasma Samples ◽  
Content Type ◽  
Transmission Setting ◽  
Merozoite Antigens ◽  
Reduced Risk

ABSTRACTPlasmodium falciparuminfections are serious in pregnant women, because VAR2CSA allows parasitized erythrocytes to sequester in the placenta, causing placental malaria (PM). In areas of endemicity, women have substantial malarial immunity prior to pregnancy, including antibodies to merozoite antigens, but produce antibodies to VAR2CSA only during pregnancy. The current study sought to determine the importance of antibodies to VAR2CSA and merozoite antigens in pregnant women in Yaoundé, Cameroon, where malaria transmission was relatively low. A total of 1,377 archival plasma samples collected at delivery were selected (at a 1:3 ratio of PM-positive [PM+] to PM-negative [PM−] women) and screened for antibodies to full-length VAR2CSA and 7 merozoite antigens. Results showed that many PM+ women and most PM− women lacked antibodies to VAR2CSA at delivery. Among PM+ women, antibodies to VAR2CSA were associated with a reduced risk of having high placental parasitemia (odds ratio [OR], 0.432; confidence interval [CI], 0.272, 0.687;P= 0.0004) and low-birth-weight (LBW) babies (OR = 0.444; CI, 0.247, 0.799;P= 0.0068), even during first pregnancies. Among antibodies to the 7 merozoite antigens, i.e., AMA1, EBA-175, MSP142, MSP2, MSP3, MSP11, and Pf41, only antibodies to MSP3, EBA-175, and Pf41 were associated with reduced risk for high placental parasitemias (P= 0.0389, 0.0291, and 0.0211, respectively) and antibodies to EBA-175 were associated with reduced risk of premature deliveries (P= 0.0211). However, after adjusting for multiple comparisons significance declined. Thus, in PM+ women, antibodies to VAR2CSA were associated with lower placental parasitemias and reduced prevalence of LBW babies in this low-transmission setting.

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