Maternal Metals/Metalloid Blood Levels Are Associated With Lipidomic Profiles Among Pregnant Women in Puerto Rico

Background/Aim: The association between heavy metal exposure and adverse birth outcomes is well-established. However, there is a paucity of research identifying biomarker profiles that may improve the early detection of heavy metal-induced adverse birth outcomes. Because lipids are abundant in our body and associated with important signaling pathways, we assessed associations between maternal metals/metalloid blood levels with lipidomic profiles among 83 pregnant women in the Puerto Rico PROTECT birth cohort.Methods: We measured 10 metals/metalloid blood levels during 24–28 weeks of pregnancy. Prenatal plasma lipidomic profiles were identified by liquid chromatography–mass spectrometry-based shotgun lipidomics. We derived sums for each lipid class and sums for each lipid sub-class (saturated, monounsaturated, polyunsaturated), which were then regressed on metals/metalloid. False discovery rate (FDR) adjusted p-values (q-values) were used to account for multiple comparisons.Results: A total of 587 unique lipids from 19 lipid classes were profiled. When controlling for multiple comparisons, we observed that maternal exposure to manganese and zinc were negatively associated with plasmenyl-phosphatidylethanolamine (PLPE), particularly those containing polyunsaturated fatty acid (PUFA) chains. In contrast to manganese and zinc, arsenic and mercury were positively associated with PLPE and plasmenyl-phosphatidylcholine (PLPC).Conclusion: Certain metals were significantly associated with lipids that are responsible for the biophysical properties of the cell membrane and antioxidant defense in lipid peroxidation. This study highlighted lipid-metal associations and we anticipate that this study will open up new avenues for developing diagnostic tools.

Objective Eye Tracking Metrics of Vision and Autonomic Dysfunction Distinguish Adolescents With Acute Concussion and Those with Persistent Post-Concussion Symptoms from Uninjured Controls

ObjectiveTo explore the utility of an eye-tracking assessment in distinguishing binocular alignment, saccadic movement, and pupillary dynamics among uninjured adolescents, acute cases (= 28 days since concussion), and persistent cases (>28 days since concussion).BackgroundVisual and autonomic system disturbances are common sequelae of concussion. Quantification of visual and autonomic dysfunction via an eye tracking device could provide an objective method of acute diagnosis and subacute identification of ongoing injury.Design/MethodsWe compared 347 eye tracking metrics, derived from a 220 seconds eye-tracking assessment, among 132 uninjured adolescents (mean age: 15.3, 56.2% female), 110 acute cases (mean days since injury: 12.5, mean age: 15.4, 46.4% female), and 95 persistent cases (mean days since injury: 53.6, mean age: 15.4, 70.2% female) using Kruskal-Wallis tests with Bonferroni corrections to account for multiple comparisons.ResultsNine eye-tracking metrics were significantly associated with injury status. One measure of binocular alignment (acute v. control: p = 0.003, persistent v. control: p = 0.001) and one measure of saccadic movement (acute v. persistent: p = 0.03, acute v. control: p = 0.03, persistent v. control: p < 0.001) were worse in cases. Cases had larger left and right mean and median pupil size than uninjured adolescents (acute v. control, p < 0.001; persistent v. control, p < 0.001). Cases had greater differences in mean (acute v. control: p < 0.001, persistent v. control: p < 0.001), median (acute v. control, p < 0.001, persistent v. control, p = 0.003), and variance of (acute v. control: p < 0.001, persistent v. control: p < 0.001) left and right pupil size. Eight of these metrics distinguished female cases from uninjured adolescents, but similar differences were not observed in male participants.ConclusionsObjective eye tracking technology can identify vision and pupillary disturbances after concussion. These metrics could be integrated into clinical practice to monitor recovery in a heterogeneous adolescent concussion population and may identify sex-specific differences in autonomic dysfunction.

Multiple comparisons of precipitation variations in different areas using simultaneous confidence intervals for all possible ratios of variances of several zero-inflated lognormal models

Flash flooding and landslides regularly cause injury, death, and homelessness in Thailand. An advancedwarning system is necessary for predicting natural disasters, and analyzing the variability of daily precipitation might be usable in this regard. Moreover, analyzing the differences in precipitation data among multiple weather stations could be used to predict variations in meteorological conditions throughout the country. Since precipitation data in Thailand follow a zero-inflated lognormal (ZILN) distribution, multiple comparisons of precipitation variation in different areas can be addressed by using simultaneous confidence intervals (SCIs) for all possible pairwise ratios of variances of several ZILN models. Herein, we formulate SCIs using Bayesian, generalized pivotal quantity (GPQ), and parametric bootstrap (PB) approaches. The results of a simulation study provide insight into the performances of the SCIs. Those based on PB and the Bayesian approach via probability matching with the beta prior performed well in situations with a large amount of zero-inflated data with a large variance. Besides, the Bayesian based on the reference-beta prior and GPQ SCIs can be considered as alternative approaches for small-to-large and medium-to-large sample sizes from large population, respectively. These approaches were applied to estimate the precipitation variability among weather stations in lower southern Thailand to illustrate their efficacies.

Fear and Anxiety Caused by COVID-19 in the Iranian Society and the Influential Factors

Background: Due to the changing nature of the spread of emerging infectious diseases, such crises could cause significant fear, especially when the disease is associated with high mortality. Fear and anxiety adversely affect health. Objectives: The present study aimed to investigate the fear and anxiety caused by COVID-19 in the Iranian society and the influential factors in this regard. Methods: This correlational study was conducted on 458 participants who were recruited for an online survey. Data were collected using a researcher-made questionnaire of COVID-19 fear and Beck's anxiety inventory. Data analysis was performed in SPSS version 23 using descriptive statistics (frequency, percentage, mean, and standard deviation) and inferential statistics, including t-test and multiple comparisons. Results: The prevalence of fear and anxiety was less than 20%. The correlations between the anxiety scores and fear of COVID-19 with demographic variables indicated that the COVID-19 fear scores had a weak correlation with an appeal to religion and efforts to prevent COVID-19 (P ≤ 0.05), while no correlation was observed with the other variables. In addition, the anxiety scores had weak, inverse correlations with the effort to prevent COVID-19 and satisfaction with the government’s effort (P ≤ 0.05). Conclusions: According to the results, the fear of COVID-19 and the subsequent anxiety is mild in the Iranian society. Low anxiety and fear caused by the disease could lead to negligence and disregarding health standards, which will increase the number of these patients in the community.

Testing the Efficacy of ‘Unlearning’, a Mindfulness and Compassion-Based Programme for Cultivating Nonviolence in Teenagers: A Randomised Controlled Trial

Background: Most programmes developed to reduce aggressive attitudes among teenagers are based on cultivating nonviolence, a construct that has been related to compassion and, more indirectly, mindfulness. This study aims at testing the efficacy of ‘Unlearning’, a mindfulness and compassion-based programme, for reducing aggressive attitudes in adolescents.Method: A sample of 164 students from three high schools in Zaragoza (Spain) participated in the study. They were randomly assigned to (1) ‘Unlearning’, or (2) relaxation programme. Three assessment points were established: baseline, post-treatment and a 4-month follow-up. The outcome variables were the subscales of the ‘Attitudes Toward Social Aggression Scale’. Mindfulness and compassion were assessed as secondary outcomes.Results: ‘Unlearning’ did not produce changes in the primary outcomes, but significant effects were observed post-treatment in self-compassion; and in the follow-up, in self-compassion and mindfulness. The control group did not experience any change post-treatment, but a significant effect in mindfulness was observed in the follow-up. The intergroup analyses indicated that ‘Unlearning’ improved self-compassion, both post-treatment (t = −2.48, p = 0.014) and after 4-months (t = −2.03, p = 0.044), although these results were not statistically significant after correcting for multiple comparisons.Conclusion: ‘Unlearning’ did not produce significant reductions in aggressive attitudes compared to the control group. The low baseline levels may have hindered the efficacy of the interventions. ‘Unlearning’ showed potential to improve self-compassion, which is related to nonviolence, and this may have positive implications for the adolescents. Future interventions should include teachers and families to enhance the effectiveness of the programmes.

An Algorithm for Evaluating the Results of Statistical Analysis of Biomedical Data under the Conditions of the Effect of Multiple Comparisons

The paper proposes an algorithm for assessing the significance of differences in the problem of managing research design under the conditions of the effect of multiple comparisons. Using the example of the problem of comparing data coming from paired channels of an electroencephalograph, the application of the algorithm is described.

Dimensional stability of stored extended-pour irreversible hydrocolloids materials

The purpose of this study was to evaluate the linear dimensional stability of four extended-pour irreversible hydrocolloids (EPIHs). Material and Methods: Five samples per material (Cavex ColorChange, Cavex Orthotrace, Jeltrate Plus, and Orthoprint) were prepared following the manufacturers’ instructions. The samples were prepared using a cylindrical matrix coupled with a nylon-polyamide ring. Two parallel, 25-mm equidistant lines were made on its surface following ANSI/American Dental Association (ADA) Specification 18 for plaster reproducibility and compatibility and Specification 19 for linear dimensional change. The samples were stored in an environment with a relative humidity of 70% (± 3) and temperature of 28°C (± 2). Photo images were obtained using a digital camera to record images for 120 hours, with a standardized distance of 80cm between the lens and the specimen. Adobe Photoshop CS3 software was used for the measurement of the recorded images. The measurements refer to the equivalent distance between the two parallel lines printed on the samples. Data were analyzed using one-way analysis of variance (ANOVA) and Tukey’s test for multiple comparisons between the means of the groups. Results: There was no statistically significant difference (p > 0.05) when EPIHs were compared at the same time of evaluation. Orthoprint, Cavex Orthotrace, and Cavex Colorchange presented with least dimensional stability up to 24 hours (p > 0.05) of storage, followed by Jeltrate Plus (48 hours). Conclusions: Storage of EPIHs for more than 24 hours for Cavex ColorChange and 48 hours for others EPIHs studied produces significant dimensional changes in the impressions stored at a humidity of 70% (± 3) and temperature of 28°C (± 2). Extended storage times produce large dimensional changes.

Photosensitization of a subcutaneous tumour by the natural anthraquinone parietin and blue light

Photodynamic therapy (PDT) is an anticancer treatment involving administration of a tumour-localizing photosensitizer, followed by activation by light of a suitable wavelength. In previous work, we showed that the natural anthraquinone (AQ) Parietin (PTN), was a promising photosensitizer for photodynamic therapy of leukemic cells in vitro. The present work aimed to analyze the photosensitizing ability of PTN in the mammary carcinoma LM2 cells in vitro and in vivo in a model of subcutaneously implanted tumours. Photodynamic therapy mediated by parietin (PTN-PDT) (PTN 30 µM, 1 h and 1.78 J/cm2 of blue light) impaired cell growth and migration of LM2 cells in vitro. PTN per se induced a significant decrease in cell migration, and it was even more marked after illumination (migration index was 0.65 for PTN and 0.30 for PTN-PDT, *p < 0.0001, ANOVA test followed by Tukey’s multiple comparisons test), suggesting that both PTN and PTN-PDT would be potential inhibitors of metastasis. Fluorescence microscopy observation indicated cytoplasmic localization of the AQ and no fluorescence at all was recorded in the nuclei. When PTN (1.96 mg) dissolved in dimethyl sulfoxide was topically applied on the skin of mice subcutaneously implanted with LM2 cells, PTN orange fluorescence was strongly noticed in the stratum corneum and also in the inner layers of the tumour up to approximately 5 mm. After illumination with 12.74 J/cm2 of blue light, one PDT dose at day 1, induced a significant tumour growth delay at day 3, which was not maintained in time. Therefore, we administered a second PTN-PDT boost on day 3. Under these conditions, the delay of tumour growth was 28% both on days 3 and 4 of the experiment (*p < 0.05 control vs. PTN-PDT, two-way ANOVA, followed by Sidak’s multiple comparisons test). Histology of tumours revealed massive tumour necrosis up to 4 mm of depth. Intriguingly, a superficial area of viable tumour in the 1 mm superficial area, and a quite conserved intact skin was evidenced. We hypothesize that this may be due to PTN aggregation in contact with the skin and tumour milieu of the most superficial tumour layers, thus avoiding its photochemical properties. On the other hand, normal skin treated with PTN-PDT exhibited slight histological changes. These preliminary findings encourage further studies of natural AQs administered in different vehicles, for topical treatment of cutaneous malignancies.

The associations between infertility-related stress, family adaptability and family cohesion in infertile couples

To explore the association between infertility-related stress, family adaptability and family cohesion in infertile couples and the determinants of infertility-related stress in infertile couples. Fertility Problem Inventory (FPI) and Family Adaptability and Cohesion Evaluation Scales (FACESII-CV) were used to measure the infertility-related stress and family adaptability and cohesion of infertile couples. T-test, ANOVA and multiple comparisons (LSD) were conducted to compare the FPI scores of different demographic characteristics subgroups. Stepwise multivariate linear regression was used to explore the determinants of infertility-related stress. Women had greater global stress than men (P < 0.001). Women scored higher on desired family adaptability, cohesion dissatisfaction and adaptive dissatisfaction than men (P = 0.039, P = 0.036, P = 0.008). FPI scores were higher in men and women who lived in rural (P < 0.001, P < 0.001). Family cohesion and education level was negatively correlated with infertility-related stress in men. Family adaptability and education level was negatively correlated with infertility-related stress in women. Healthcare providers should pay more attention and give more support to infertile couples who lived in rural or with low education level, and provide easier medical accessing for them. Moreover, healthcare providers should value more the family function and family support in intervention of reducing infertility-related stress.