Two-dimensional electrophoresis of cerebrospinal fluid proteins in normal and pathological conditions

1985 ◽  
Vol 10 (9) ◽  
pp. 1203-1219 ◽  
Author(s):  
F. Bracco ◽  
P. Gallo ◽  
B. Tavolato ◽  
L. Battistin
Keyword(s):  
Cerebrospinal Fluid ◽  
Two Dimensional ◽  
Two Dimensional Electrophoresis ◽  
Cerebrospinal Fluid Proteins ◽  
Pathological Conditions ◽  
Dimensional Electrophoresis

Two-dimensional electrophoresis of cerebrospinal fluid proteins in multiple sclerosis and various neurological diseases

1984 ◽  
Vol 5 (4) ◽  
pp. 236-245 ◽  
Author(s):  
Michael G. Harrington ◽  
Cari R. Merril ◽  
David Goldman ◽  
Xian-Hau Xu ◽  
Dale E. McFarlin
Keyword(s):  
Multiple Sclerosis ◽  
Cerebrospinal Fluid ◽  
Neurological Diseases ◽  
Two Dimensional ◽  
Two Dimensional Electrophoresis ◽  
Cerebrospinal Fluid Proteins ◽  
Dimensional Electrophoresis

Enhancement techniques for detecting trace and fluid-specific components in two-dimensional electrophoresis patterns.

1982 ◽  
Vol 28 (4) ◽  
pp. 759-765 ◽  
Author(s):  
G B Dermer ◽  
L M Silverman ◽  
J F Chapman
Keyword(s):  
Cerebrospinal Fluid ◽  
Serum Proteins ◽  
Prostatic Acid Phosphatase ◽  
Two Dimensional ◽  
Cibacron Blue ◽  
Two Dimensional Electrophoresis ◽  
Prostatic Fluid ◽  
Creatine Kinase B ◽  
Malignant Effusions ◽  
Dimensional Electrophoresis

Abstract Albumin and other serum-derived proteins were removed from several types of body fluids by affinity chromatography, to facilitate detection of trace or non-serum-derived proteins in two-dimensional electrophoresis patterns. Albumin was removed by the dye Cibacron Blue F3G-A coupled to Sepharose. Two-dimensional patterns of albumin-depleted serum lack the large albumin spot, and several families of spots become visible that ordinarily are partly or totally hidden by it. However, other proteins also bind to Cibacron Blue. Most serum proteins, including albumin, were effectively removed by anti-human serum antibodies coupled to Sepharose. Two-dimensional patterns of serum-depleted cerebrospinal fluid exhibit five clusters of probable nervous-system protein families not detected in serum. One additional family, probably antigenically related to transferrin, was removed by the affinity step. Two-dimensional patterns of serum-depleted prostatic fluid exhibit five major non-serum families, two of which may be creatine kinase B subunits and prostatic acid phosphatase. Two-dimensional patterns of serum-depleted malignant effusions exhibit one or more of three proteins that possibly are tumor products. Pattern matching suggests the presence of one non-serum-derived protein family common to cerebrospinal fluid, prostatic fluid, and malignant effusions. Prostatic fluid and malignant effusions have in common as many as three non-serum families of proteins.

Assessing cerebrospinal fluid rhinorrhea: A two-dimensional electrophoresis approach

2001 ◽  
Vol 22 (9) ◽  
pp. 1826-1833 ◽  
Author(s):  
Pierre R. Burkhard ◽  
Neftali Rodrigo ◽  
Daniel May ◽  
Roman Sztajzel ◽  
Jean-Charles Sanchez ◽  
...  
Keyword(s):  
Cerebrospinal Fluid ◽  
Two Dimensional ◽  
Cerebrospinal Fluid Rhinorrhea ◽  
Two Dimensional Electrophoresis ◽  
Dimensional Electrophoresis

Microscale multisample two-dimensional electrophoresis of proteins in human serum, cerebrospinal fluid, and urine.

1982 ◽  
Vol 28 (4) ◽  
pp. 824-827 ◽  
Author(s):  
T Manabe ◽  
E Hayama ◽  
T Okuyama
Keyword(s):  
Cerebrospinal Fluid ◽  
Human Serum ◽  
Distribution Patterns ◽  
Blue Staining ◽  
Two Dimensional ◽  
Protein Distribution ◽  
Two Dimensional Electrophoresis ◽  
Coomassie Blue Staining ◽  
Coomassie Blue ◽  
Dimensional Electrophoresis

Abstract In this technique, in which no denaturing agent is used, proteins in human serum, cerebrospinal fluid, and urine are separated by isoelectric focusing in cylindrical 40 g/L polyacrylamide gels of capillary size (1.3 x 35 mm) for 40 min, followed by electrophoresis in 40--170 g/L polyacrylamide linear gradient gel, with use of 38 x 35 x 1 mm slab gel, for 1 h. Only 2 microL of untreated human serum is required to obtain clear protein-distribution patterns, made visible by Coomassie Blue staining. By use of silver staining, proteins in unconcentrated cerebrospinal fluid can be made visible. An apparatus we devised for microscale two-dimensional electrophoresis enables us to analyze eight protein samples simultaneously.

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