Glycosphingolipids in feces of germ-free rats as a source for studies of developmental changes of intestinal epithelial cell surface carbohydrates

1989 ◽  
Vol 6 (3) ◽  
pp. 285-292 ◽  
Author(s):  
G�ran Larson ◽  
Tore Midtvedt
Keyword(s):  
Epithelial Cell ◽  
Cell Surface ◽  
Intestinal Epithelial Cell ◽  
Developmental Changes ◽  
Intestinal Epithelial ◽  
Germ Free ◽  
Epithelial Cell Surface ◽  
Surface Carbohydrates

Intestinal Epithelial Cell Surface Glycosylation in Mice

1992 ◽  
Vol 36 (2) ◽  
pp. 71-78 ◽  
Author(s):  
Reena Gupta ◽  
Virender M.S. Jaswal ◽  
Akhtar Mahmood
Keyword(s):  
Epithelial Cell ◽  
Cell Surface ◽  
Intestinal Epithelial Cell ◽  
Intestinal Epithelial ◽  
Epithelial Cell Surface

Intestinal Epithelial Cell Surface Glycosylation in Mice

1992 ◽  
Vol 36 (5-6) ◽  
pp. 288-295 ◽  
Author(s):  
Reena Gupta ◽  
Virender M.S. Jaswal ◽  
Meenu Akhtar Mahmood
Keyword(s):  
Epithelial Cell ◽  
Cell Surface ◽  
Intestinal Epithelial Cell ◽  
Intestinal Epithelial ◽  
Epithelial Cell Surface

scholarly journals Immunohistochemical and biochemical demonstration of the change in glycolipid composition of the intestinal epithelial cell surface in mice in relation to epithelial cell differentiation and bacterial association.

1984 ◽  
Vol 32 (3) ◽  
pp. 299-304 ◽  
Author(s):  
Y Umesaki
Keyword(s):  
Epithelial Cell ◽  
Epithelial Cells ◽  
Intestinal Epithelial Cell ◽  
Small Intestinal Mucosa ◽  
Intestinal Epithelial ◽  
Germ Free ◽  
Microvillus Membrane ◽  
Small Intestinal ◽  
Asialo Gm1 ◽  
Crypt Cells

We have previously demonstrated the appearance of fucosyl asialo-GM1 (FGA1) in the small-intestinal epithelial cells of germ-free mice via the induction of GDP-fucose: asialo-GM1 (GA1) alpha(1 leads to 2) fucosyltransferase (FT) after the conventionalization of these animals (Umesaki Y, Sakata T, Yajima T: Biochem Biophys Res Commun 105:439, 1982). The present study, based on this earlier work, demonstrates the changes in the glycolipid antigens of the small-intestinal epithelial-cell membrane as shown immunohistochemically with specific antibodies raised against asialo GM1 (GA1) and FGA1. In germ-free mice, GA1 was localized both in the villus cells and in the crypt cells. In the process of conventionalization, FGA1 appeared in the villus cells while the GA1 content of these cells was decreased. Four to 5 days after the conventionalization procedure, the fluorescence produced by anti-FGA1 was strongest in the villus cells, while that produced by anti-GA1 was detected only in the crypt cells. At this same time the FT activity of the small-intestinal mucosa was highest, with most of the GA1 apparently being converted into FGA1, as shown in the paper cited above. Thereafter, the GA1 content of both the villus and crypt cells again increased greatly. On the other hand, the fluorescence produced with anti-FGA1 decreased, and could no longer be detected 14 days after conventionalization. The activity of FT, measured biochemically in epithelial cells differentially isolated from the villus tip to the crypt, was greater in the villus than in the crypt region. This confirmed the intense staining with anti-FGA1 that was seen in villus cells. The fluorescence produced by the two anti-glycolipid antibodies used in the study distributed not only in the microvillus membrane but also to some extent in the basolateral membrane. The localization of the respective glycolipids contrasted with that of the glycoprotein sucrase--isomaltase enzyme complex, the fluorescence of which was exclusively confined to the microvillus-membrane side of the villus cells.

scholarly journals Does dietary fibre stimulate intestinal epithelial cell proliferation in germ free rats?

Gut ◽  
1989 ◽  
Vol 30 (6) ◽  
pp. 820-825 ◽  
Author(s):  
R A Goodlad ◽  
B Ratcliffe ◽  
J P Fordham ◽  
N A Wright
Keyword(s):  
Cell Proliferation ◽  
Epithelial Cell ◽  
Dietary Fibre ◽  
Intestinal Epithelial Cell ◽  
Intestinal Epithelial ◽  
Epithelial Cell Proliferation ◽  
Germ Free

scholarly journals Loss of epithelial cell surface carbohydrates during experimental oral carcinogenesis in the rat

1987 ◽  
Vol 55 (6) ◽  
pp. 633-638 ◽  
Author(s):  
SS Prime ◽  
TJ Rosser ◽  
LS Davies ◽  
C Scully
Keyword(s):  
Epithelial Cell ◽  
Cell Surface ◽  
Oral Carcinogenesis ◽  
Epithelial Cell Surface ◽  
Surface Carbohydrates

scholarly journals Characteristic Changes in Cell Surface Glycosylation Accompany Intestinal Epithelial Cell (IEC) Differentiation: High Mannose Structures Dominate the Cell Surface Glycome of Undifferentiated Enterocytes

2015 ◽  
Vol 14 (11) ◽  
pp. 2910-2921 ◽  
Author(s):  
Dayoung Park ◽  
Kristin A. Brune ◽  
Anupam Mitra ◽  
Alina I. Marusina ◽  
Emanual Maverakis ◽  
...  
Keyword(s):  
Epithelial Cell ◽  
Cell Surface ◽  
Intestinal Epithelial Cell ◽  
Intestinal Epithelial ◽  
High Mannose

scholarly journals Microbiota From Colon Cancer Patients Induces Intestinal Epithelial Cell Proliferation in Germ-Free Mice

2011 ◽  
Vol 140 (5) ◽  
pp. S-8
Author(s):  
Jarrousse Veronique ◽  
Sandra Guilmeau ◽  
Blugeon Sebastien ◽  
Jean-Pierre Furet ◽  
Roperch Jean-Pierre ◽  
...  
Keyword(s):  
Colon Cancer ◽  
Cell Proliferation ◽  
Epithelial Cell ◽  
Cancer Patients ◽  
Intestinal Epithelial Cell ◽  
Intestinal Epithelial ◽  
Epithelial Cell Proliferation ◽  
Germ Free
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