Evidence for electroneutral sodium chloride cotransport in the cortical thick ascending limb of Henle's loop of rabbit kidney

1983 ◽  
Vol 396 (4) ◽  
pp. 308-314 ◽  
Author(s):  
Rainer Greger ◽  
Eberhard Schlatter ◽  
Florian Lang
Keyword(s):  
Sodium Chloride ◽  
Rabbit Kidney ◽  
Thick Ascending Limb ◽  
Henle's Loop

Sodium-chloride transport in the thick ascending limb of Henle's loop

1981 ◽  
Vol 389 (3) ◽  
pp. 263-270 ◽  
Author(s):  
Jill Eveloff ◽  
Ekkehard Bayerd�rffer ◽  
Patricio Silva ◽  
Rolf Kinne
Keyword(s):  
Sodium Chloride ◽  
Chloride Transport ◽  
Thick Ascending Limb ◽  
Henle's Loop

Characterization of Aldose Reductase from the Thick Ascending Limb of Henle’s Loop of Rabbit Kidney

Nephron ◽  
2001 ◽  
Vol 89 (1) ◽  
pp. 73-81 ◽  
Author(s):  
R. Willi Grunewald ◽  
Angela Eckstein ◽  
Claudius H. Reisse ◽  
Gerhard A. Müller
Keyword(s):  
Aldose Reductase ◽  
Rabbit Kidney ◽  
Thick Ascending Limb ◽  
Henle's Loop

A potassium channel in the apical membrane of rabbit thick ascending limb of Henle's loop

1990 ◽  
Vol 258 (2) ◽  
pp. F244-F253 ◽  
Author(s):  
W. H. Wang ◽  
S. White ◽  
J. Geibel ◽  
G. Giebisch
Keyword(s):  
Apical Membrane ◽  
Rabbit Kidney ◽  
K Channel ◽  
Thick Ascending Limb ◽  
Dependent Manner ◽  
Patch Clamp Technique ◽  
Bath Solution ◽  
Henle's Loop ◽  
Voltage Dependent ◽  
Inside Out

We used the patch-clamp technique to study the activity of single potassium channels in the apical membrane of isolated thick ascending limbs of Henle's loop (TAL) of rabbit kidneys. In cell-attached patches with NaCl Ringer or high-K+ solution in the bath and 140 mM K+ in the pipette, an inwardly rectifying K+ channel was observed with an inward slope conductance of 22.0 +/- 0.5 pS and outward slope conductance of 10.2 +/- 0.3 pS at 22 degrees C (n = 15). The channel was highly selective for K+, with a calculated permeability ratio for K(+)-to-Na+ of 20:1 (n = 4). The open probability (Po) of the channel was 0.89 +/- 0.03 (n = 15) and was not voltage dependent. In inside-out patches with 140 mM K+ in both the bath and the pipette solutions, both Po and conductance of the channel were similar to that in cell-attached patches. Addition of 0.1 mM Ba2+ to the pipette solution reduced Po of the channel in a voltage-dependent manner. Lowering the pH of the bath solution from 7.4 to 6.9 or increasing Ca2+ concentration from 0 to 0.5 mM in inside-out patches did not alter either Po or conductance of the channel. Addition of 2 mM ATP to the bath solution completely inhibited channel activity. This ATP-induced inhibition was fully reversible and was found to be dependent on the ratio of ATP to ADP, since adding 1 mM ADP to the bath solution relieved the ATP-induced blockade. The property of this small-conductance K+ channel make it a likely candidate for recycling of K+ across the apical membrane of TAL of the rabbit kidney. ATP and ADP are possible intracellular regulators of the channel's activity.

A cytotoxin of pseudomonas aeruginosa acts directly on the cortical thick ascending limb of henle's loop of rabbit kidney

1982 ◽  
Vol 394 (3) ◽  
pp. 271-273 ◽  
Author(s):  
R. N. Weiner ◽  
R. Greger ◽  
E. Schlatter ◽  
F. Papavassiliou ◽  
K. J. Ullrich
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Rabbit Kidney ◽  
Thick Ascending Limb ◽  
Henle's Loop

scholarly journals Separation of renal medullary cells: isolation of cells from the thick ascending limb of Henle's loop.

1980 ◽  
Vol 87 (3) ◽  
pp. 672-681 ◽  
Author(s):  
J Eveloff ◽  
W Haase ◽  
R Kinne
Keyword(s):  
Oxygen Consumption ◽  
Adenylate Cyclase ◽  
Collecting Duct ◽  
Single Cells ◽  
Rabbit Kidney ◽  
Thick Ascending Limb ◽  
Apical Surface ◽  
Dependent Manner ◽  
Homogeneous Population ◽  
Henle's Loop

A homogeneous population of single cells from the thick ascending limb of Henle's loop (TALH) has been isolated from the rabbit kidney medulla. A total medullary cell suspension was prepared by a series of collagenase, hyaluronidase, and trypsin digestions and separated on a Ficoll gradient (2.6-30.7% wt/wt). Morphologically, the cells isolated from the TALH were homogeneous and showed polarity within their plasma membrane structure, with a few blunt microvilli on their apical surface and deep infoldings of the basal-lateral membrane. Biochemically, the TALH cells were highly enriched in calcitonin-sensitive adenylate cyclase and Na, K-ATPase. Alkaline phosphatase and arginine vasopressin-sensitive adenylate cyclase, highly concentrated in proximal tubule and collecting duct, were present only in low concentrations in the TALH cells. Additionally, furosemide, a diuretic inhibiting sodium chloride transport in the TALH in vivo, inhibited oxygen consumption of the TALH cells in a dose-dependent manner. The TALH cells were viable, as judged by morphological appearance, trypan blue exclusion, the response of oxygen consumption to 2,4-dinitrophenol, succinate and ouabain, and the cellular Na, K and ATP levels.

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