Sodium-chloride transport in the thick ascending limb of Henle's loop

1981 ◽  
Vol 389 (3) ◽  
pp. 263-270 ◽  
Author(s):  
Jill Eveloff ◽  
Ekkehard Bayerd�rffer ◽  
Patricio Silva ◽  
Rolf Kinne
Keyword(s):  
Sodium Chloride ◽  
Chloride Transport ◽  
Thick Ascending Limb ◽  
Henle's Loop

Effects of arginine vasopressin on the thin ascending limb of Henle's loop of hamsters

1982 ◽  
Vol 243 (2) ◽  
pp. F167-F172 ◽  
Author(s):  
M. Imai ◽  
E. Kusano
Keyword(s):  
Adenylate Cyclase ◽  
Arginine Vasopressin ◽  
Chloride Transport ◽  
Cyclase Activity ◽  
Adenylate Cyclase Activity ◽  
Renal Tubules ◽  
Thick Ascending Limb ◽  
Cl Transport ◽  
Henle's Loop ◽  
Perfusion Studies

Arginine vasopressin (AVP) has been shown to stimulate active Cl transport across the medullary thick ascending limb of Henle's loop (MAL) in association with an increase in adenylate cyclase activity. To determine whether the failure to demonstrate active Cl transport across the thin ascending limb of Henle's loop (TAL) in previous in vitro perfusion studies was due to the absence of AVP in the preparation, we examined the effect of AVP on adenylate cyclase activity and Cl transport in the hamsters TAL. AVP (1 mU/ml) increased adenylate cyclase activity in the hamster TAL (20.7 +/- 5.2 control vs. 46.2 +/- 10.1 fmol . mm-1 . 30 min-1, n = 6, P less than 0.05) but not in the descending limb (27.8 +/- 7.0 control vs. 20.4 +/- 2.7, n = 4, P less than 0.05). When both MAL and TAL were perfused, a lumen-positive transepithelial voltage (Vt) was observed. The Vt was increased by adding 1 or 10 mU/ml AVP to the bath. When only the TAL was perfused, the Vt was not different from zero. Similar results were obtained in mouse renal tubules. In other experiments, AVP did not affect the diffusion potential generated when a transepithelial NaCl gradient was present. AVP or dibutyryl cAMP caused little or no change in efflux of radioactive chloride across the hamster TAL. These findings suggest that electrogenic chloride transport is not demonstrable in the TAL even in the presence of AVP. The physiologic role of AVP-sensitive adenylate cyclase in the TAL remains to be established.

Diluting segment in avian kidney. II. Water and chloride transport

1986 ◽  
Vol 250 (3) ◽  
pp. R341-R347 ◽  
Author(s):  
T. Miwa ◽  
H. Nishimura
Keyword(s):  
Water Permeability ◽  
Chloride Transport ◽  
Arginine Vasotocin ◽  
Osmotic Gradient ◽  
Thick Ascending Limb ◽  
Osmotic Water Permeability ◽  
Diluting Segment ◽  
Henle's Loop ◽  
Osmotic Water ◽  
Avian Kidney

The mammalian-type nephrons of avian kidneys contain a Henle's loop that runs parallel to the collecting ducts and the vasa recta. Thus we examined whether the thick ascending limb (TAL) of Henle's loop of the avian kidney acts as a diluting segment by measuring water and Cl transport in the isolated and perfused TAL of the quail, Coturnix coturnix. The TAL showed a lumen-positive transepithelial voltage (Vt) (+9.4 +/- 0.4 mV, n = 28). Net water flux (Jv) was nearly zero when the TAL was perfused and bathed with isosmotic solution. When the osmotic gradient was imposed, Jv increased only slightly, and thus the osmotic water permeability (Lp) was low. Arginine vasotocin (AVT) added to the hyperosmotic bath did not alter either Jv, Lp, or Vt. Cl efflux (lumen to bath, 370.4 +/- 27.7 peq X mm-1 X min-1) was higher than Cl influx (bath to lumen, 98.6 +/- 14.3 peq X mm-1 X min-1) when measured in the different tubules. AVT showed no effect on Cl efflux. These results indicate that in the TAL of the quail osmotic water permeability is low while net Cl reabsorption is present, suggesting that the TAL functions as a diluting segment.

Importance of chloride for acute inhibition of renin by sodium chloride

1978 ◽  
Vol 235 (5) ◽  
pp. F444-F450 ◽  
Author(s):  
K. A. Kirchner ◽  
T. A. Kotchen ◽  
J. H. Galla ◽  
R. G. Luke
Keyword(s):  
Sodium Chloride ◽  
Chloride Transport ◽  
Free Water ◽  
Plasma Renin ◽  
Sodium Balance ◽  
Thick Ascending Limb ◽  
Free Water Clearance ◽  
Sodium Salts ◽  
Before And After ◽  
Renin Inhibition

To evaluate the contribution of chloride to acute renin inhibition by sodium chloride, plasma renin activity (PRA) was measured before and after peripheral venous infusion of NaCl, NaHCO3, NaBr, NaNO3, lysine monohydrochloride, or lysine glutamate in NaCl-deprived rats. In contrast to controls and animals infused with other sodium salts, PRA decreased (P less than 0.01) after infusion with NaCl [from 28.3 +/- 2.8 to 13.3 +/- 1.8 ng/ml per h (SE)] and NaBr (from 40.6 +/- 6.2 to 21.8 +/- 3.9 ng/ml per h), and renal tubular halide reabsorption increased (P less than 0.05). Arterial pressure, plasma volume, inulin clearance, net sodium balance, serum Na+ and K+, and pH were not different among sodium-loaded groups. PRA was also suppressed (P less than 0.01) by infusion with lysine monohydrochloride (from 51.6 +/- 5.4 to 32.4 +/- 5.1 ng/ml per h) but not with lysine glutamate. These results suggest that inhibition of renin by sodium is dependent on an intrarenal effect of chloride. During infusion with sodium salts which suppressed renin, negative free water clearance (TcH2O) increased, whereas infusion with sodium salts that did not inhibit renin resulted in either no change or decreased TcH2O. The association of renin inhibition and increased TcH2O indirectly supports the hypothesis that renin suppression by chloride is related to the magnitude of absorptive chloride transport in the thick ascending limb of the loop of Henle.

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