Turnover of ribosomal 28S and 18S rRNA during rat liver regeneration

The turnover of 28S and 18S rRNA was studied in the course of 12 d after partial hepatectomy, including the proliferative (1st to 5th d) and post-proliferative (6th to 12th d) phases of liver regeneration. Turnover data, as the day-to-day rates of synthesis and degradation of 28S and 18S rRNA, were obtained by employing a suitable experimental procedure for the estimation of the increase of the amount of rRNA in the regenerating liver. It was found that 28S and 18S rRNA are accumulated into the cytoplasm and degraded at identical rates both in the proliferative and post proliferative phases. The turnover of both rRNA moieties is markedly slower during the first 3 d of liver regeneration.

Download Full-text

Enhanced production of dolichol, but not dolichyl phosphate, in the earliest stages of rat liver regeneration

Bioscience Reports ◽

10.1007/bf01116429 ◽

1986 ◽

Vol 6 (4) ◽

pp. 409-413 ◽

Cited By ~ 16

Author(s):

M. Marino ◽

G. Bruscalupi ◽

S. Spagnuolo ◽

S. Leoni ◽

M. T. Mangiantini ◽

...

Keyword(s):

Liver Regeneration ◽

Partial Hepatectomy ◽

Rat Liver ◽

Physiological Significance ◽

Regenerating Liver ◽

Rat Liver Regeneration ◽

Enhanced Production ◽

Dolichyl Phosphate

The regenerating liver presents a changed ability to use mevalonate 16 hr after partial hepatectomy. The dolichol content and its synthesis from mevalonate is increased, while no variation of dolichyl-P and ubiquinone parameters are detectable. The greater amount ofmevalonate utilized to form dolichol, but not dolichyl-P, in this proliferating system, raises some questions about the physiological significance of these isoprenoid compounds and about their biosynthetic sequence.

Download Full-text

DEOXYCYTIDYLATE DEAMINASE LEVELS IN REGENERATING RAT LIVER

Canadian Journal of Biochemistry and Physiology ◽

10.1139/o61-105 ◽

1961 ◽

Vol 39 (6) ◽

pp. 1043-1054 ◽

Cited By ~ 24

Author(s):

D. K. Myers ◽

C. Anne Hemphill ◽

Constance M. Townsend

Keyword(s):

Dna Synthesis ◽

Liver Regeneration ◽

Partial Hepatectomy ◽

Rat Liver ◽

Deoxyribonucleic Acid ◽

Regenerating Liver ◽

Regenerating Rat Liver ◽

High Level ◽

Early Regeneration

Deoxycytidylate deaminase activity and net synthesis of deoxyribonucleic acid (DNA) in vivo were found to increase at approximately the same time during the early stages of liver regeneration. However, deaminase activity in the regenerating liver remained at a high level for 1 day after DNA synthesis had slowed down again during the later stages of regeneration. The increase in deaminase activity was restricted as a result of exposure to 600 r X radiation during early regeneration, but this effect only became evident 11–16 hours after the irradiation. Irradiation on the second day after partial hepatectomy, when deaminase levels in control regenerating livers were relatively constant, failed to affect the deaminase activity immediately but did produce a 40–50% decrease in activity 11–16 hours later. Other antimitotic agents, e.g., colchicine, had little effect on deaminase activity.

Download Full-text