Alkyl ketoximes as analytical reagents: The chloroform extraction of the alkyl ketoxime complexes of palladium (II) chloride

Oyster samples processed by adsorption-elution-precipitation were seeded with poliovirus 1 and HAV, and cleaned and concentrated by Freon extraction (2X), PEG precipitation and chloroform extraction. Freon extraction resulted in recoveries of 63-76% for polio and 42-52% for HAV. PEG precipitation/chloroform extraction gave recoveries of 47-50% for polio and 15-19% for HAV. Treated extracts inhibited RT-PCR at 10−2 dilutions. Inhibitors were removed by treatment with the cationic detergent CTAB or Pro-Cipitate/UF adsorption-elution-concentration. Both treatments resulted in samples on which direct RT-PCR was possible. The CTAB procedure was able to detect 78 pfu of polio and 295 pfu of HAV. The Pro-Cipitate procedure was able to detect 70 pfu polio and 2.1×103 pfu HAV.

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ANALYTICAL REAGENTS

The Medical Journal of Australia ◽

10.5694/j.1326-5377.1926.tb40076.x ◽

1926 ◽

Vol 2 (3) ◽

pp. 84-84

Keyword(s):

Analytical Reagents

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DETERMINATION OF FREE AND TOTAL CHOLESTEROL BY DIRECT CHLOROFORM EXTRACTION

Journal of Biological Chemistry ◽

10.1016/s0021-9258(18)56747-8 ◽

1949 ◽

Vol 180 (1) ◽

pp. 315-328 ◽

Cited By ~ 3

Author(s):

George R. Kingsley ◽

Roscoe R. Schaffert

Keyword(s):

Total Cholesterol ◽

Chloroform Extraction

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N-ACYL SUBSTITUTED PHENYLHYDROXYLAMINES: THE EFFECT OF RADICAL CHANGE ON ANALYTICAL BEHAVIOR

Canadian Journal of Chemistry ◽

10.1139/v57-192 ◽

1957 ◽

Vol 35 (12) ◽

pp. 1454-1460 ◽

Cited By ~ 18

Author(s):

C. A. Armour ◽

D. E. Ryan

Keyword(s):

Aqueous Solution ◽

Stability Constant ◽

Radical Change ◽

Spectrophotometric Study ◽

Iron Complex ◽

Membered Ring ◽

Analytical Reagents ◽

Colorimetric Reagent ◽

Analytical Behavior

The synthesis of a number of N-acyl substituted phenylhydroxylamines is outlined and the effect of the change in the nature of the acyl group on their usefulness as analytical reagents is described. Stability constant measurements in alcohol show an increase in stability with an increase in basicity of reagent regardless of the nature of the attached group; their ability to precipitate complexes from aqueous solution, however, is markedly dependent on the nature of the attached radical. A spectrophotometric study shows the existence of both a 1:1 and a 1:3 iron complex. A more sensitive colorimetric reagent results with an unsaturated five-membered ring as the attached grouping.

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