Generation of a panel of somatic cell hybrids containing unselected fragments of human chromosome 10 by X-ray irradiation and cell fusion: Application to isolating theMEN2A region in hybrid cells

1990 ◽  
Vol 16 (2) ◽  
pp. 163-171 ◽  
Author(s):  
P. J. Goodfellow ◽  
S. Povey ◽  
H. A. Nevanlinna ◽  
P. N. Goodfellow
Keyword(s):  
Human Chromosome ◽  
Somatic Cell ◽  
Cell Fusion ◽  
Hybrid Cells ◽  
Somatic Cell Hybrids ◽  
Chromosome 10 ◽  
X Ray ◽  
Cell Hybrids

Generation of a panel of somatic cell hybrids containing fragments of human chromosome 12p by X-ray irradiation and cell fusion

Genomics ◽  
1992 ◽  
Vol 12 (2) ◽  
pp. 206-213 ◽  
Author(s):  
R.J. Sinke ◽  
R.F. Suijkerbuijk ◽  
J. Herbergs ◽  
H. Janssen ◽  
J.J. Cassiman ◽  
...  
Keyword(s):  
Human Chromosome ◽  
Somatic Cell ◽  
Cell Fusion ◽  
Somatic Cell Hybrids ◽  
X Ray ◽  
Cell Hybrids ◽  
Chromosome 12P

Expression of Factor VIII in Cultured Endothelial Cells and Somatic Cell Hybrids

1979 ◽  
Author(s):  
Cora-Jean S. Edgell ◽  
Howard M. Reisner ◽  
John B. Graham
Keyword(s):  
Endothelial Cells ◽  
Human Chromosome ◽  
Factor Viii ◽  
Somatic Cell ◽  
Fluorescent Antibody ◽  
Primary Cultures ◽  
Hybrid Cells ◽  
Somatic Cell Hybrids ◽  
Cell Hybrids ◽  
Cultured Endothelial Cells

Somatic cell hybrids have been produced using primary cultures of human vascular endothelial cells and rodent cell strains. Production of factor VIII related antigen (VIIIR :Ag) by the cultured endothelial cells can be clearly demonstrated by a direct fluorescent antibody assay, but has not yet been detected in 10 of 10 hybrid clones. The hybrid cells do express many other human traits. Isozyme analyses for human chromosome markers demonstrate that various human chromosomes have been segregated from each of the hybrids. There are several tenable explanations for the fact that human VIIIR:Ag has not been detected in these hybrid cells. 1) Factor VIII may require a human chromosome(s) not present in any of the hyhrids ana1yzed to date. 2) Factor VIII may be expressed at lower levels in hybrid cells such that it would be detectable only by a more sensitive assay. 3) The rodent genome may block expression of this differentiated function in the hybrid cells. Since in a whole organism, only certa in cells express VIIIR:Ag, lack of its expression in hybrid cells may be ana1agous to its regulation in vivo.

Human chromosome 16 physical map: Mapping of somatic cell hybrids using multiplex PCR deletion analysis of sequence tagged sites

Genomics ◽  
1991 ◽  
Vol 10 (4) ◽  
pp. 1047-1052 ◽  
Author(s):  
Robert I. Richards ◽  
Katherine Holman ◽  
Sharon Lane ◽  
Grant R. Sutherland ◽  
David F. Callen
Keyword(s):  
Human Chromosome ◽  
Somatic Cell ◽  
Multiplex Pcr ◽  
Physical Map ◽  
Deletion Analysis ◽  
Somatic Cell Hybrids ◽  
Chromosome 16 ◽  
Cell Hybrids ◽  
Sequence Tagged Sites

Assignment of inosine triphosphatasegene to gorilla chromosome 13 and to human chromosome 20 in primate-rodent somatic cell hybrids

1976 ◽  
Vol 16 (1-5) ◽  
pp. 420-421 ◽  
Author(s):  
Meera Khan ◽  
P.L. Pearson ◽  
L.L.L. Wijnen ◽  
B.A. Doppert ◽  
A. Westerveld ◽  
...  
Keyword(s):  
Human Chromosome ◽  
Somatic Cell ◽  
Somatic Cell Hybrids ◽  
Chromosome 13 ◽  
Cell Hybrids ◽  
Chromosome 20 ◽  
I Gene ◽  
Inosine Triphosphatase

Assignment of the human slow twitch skeletal muscle/cardiac troponin C gene (TIMNC1) to human chromosome 3p21.3→3p14.3 using somatic cell hybrids

1996 ◽  
Vol 75 (1) ◽  
pp. 36-37 ◽  
Author(s):  
W.-J Song ◽  
M.L. Van Keuren ◽  
H.A. Drabkin ◽  
J.R. Cypser ◽  
R.M. Gemmill ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Human Chromosome ◽  
Somatic Cell ◽  
Cardiac Troponin ◽  
Troponin C ◽  
Somatic Cell Hybrids ◽  
Cardiac Troponin C ◽  
Cell Hybrids ◽  
Slow Twitch

The human chromosome content in human × rodent somatic cell hybrids analyzed by a screening technique using Alu PCR

Genomics ◽  
1991 ◽  
Vol 10 (1) ◽  
pp. 186-192 ◽  
Author(s):  
David C. Bicknell ◽  
David Markie ◽  
Nigel K. Spurr ◽  
Walter F. Bodmer
Keyword(s):  
Human Chromosome ◽  
Somatic Cell ◽  
Somatic Cell Hybrids ◽  
Screening Technique ◽  
Cell Hybrids

scholarly journals The expression of several T cell-specific and novel genes is repressed by trans-acting factors in immature T lymphoma clones.

1991 ◽  
Vol 174 (1) ◽  
pp. 269-280 ◽  
Author(s):  
M F Wilkinson ◽  
J Doskow ◽  
R von Borstel ◽  
A M Fong ◽  
C L MacLeod
Keyword(s):  
T Cell ◽  
Somatic Cell ◽  
Cell Lines ◽  
Hybrid Cells ◽  
Somatic Cell Hybrids ◽  
Novel Genes ◽  
Mrna Accumulation ◽  
Cell Hybrids ◽  
T Lymphoma ◽  
Cell Ontogeny

Cell surface proteins encoded by members of the immunoglobulin supergene family are sequentially expressed during T cell ontogeny. The molecular mechanisms responsible for the regulation of these surface molecules are not well understood. To investigate this issue, we used a series of well characterized T lymphoma cell clones with phenotypes characteristic of distinct stages of early thymocyte maturation. Somatic cell hybrids formed from these cell lines were employed to detect the presence of negative regulatory molecules. The expression of CD4 and CD8 was strongly repressed in hybrids formed between a CD4+ CD8+ lymphoma clone and "immature" CD4- CD8- lymphoma clones. Individual subunits of the T cell receptor (TCR)/CD3 complex displayed independent regulation in unique patterns in hybrid cells. Hybrids formed by fusing CD3+ and CD3- cells completely repressed CD3-delta mRNA expression while CD3-gamma, -epsilon, and -zeta transcripts were moderately inhibited or codominantly regulated. Similar to CD3-delta, interleukin 2R-alpha(IL-2R-alpha), and TCR-beta mRNA accumulation was trans-negatively regulated. Transcription rate measurements demonstrated that the inhibition of CD4, CD8, CD3-gamma, CD3-epsilon, TCR-beta, and IL-2R-alpha mRNA accumulation in hybrid cells was exerted, at least in part, at the transcriptional level. To test whether repressional regulation is a general feature of T cells, we examined the regulation of six novel genes which were selected solely on the basis of their differential expression between two of the cell lines used in this study. Five of the six novel gene transcripts were repressed in the somatic cell hybrids. Thus, inhibitor factors appear to play a general role in controlling T cell gene expression. The model system presented here may be useful for the identification and characterization of repressor molecules responsible for the regulation of genes expressed during T cell ontogeny.

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