Reduced maturation of friend virus in adhesive mutants of friend leukemia cells

1987 ◽  
Vol 92 (1-2) ◽  
pp. 151-164
Author(s):  
C. Amici ◽  
C. Oppi ◽  
G. Fiorucci ◽  
A. Battistini ◽  
W. Djaczenko ◽  
...  
Author(s):  
W. Djaczenko ◽  
M. Müller ◽  
A. Benedetto ◽  
G. Carbone

A thickening of ER membranes in murine myeloma cells was attributed by de Harven to the assembly of intracisternal virus particles. We observed similar thickening of GER membranes in Friend leukemia cells (FLC) apparently associated with Friend leukemia virus (FLV) assembly. We reinvestigated the problem of GER involvement in FLV assembly using high pressure cryofixed FLC.FLC (745A clone growing in suspension and FF clone growing in monolayer) were immersed in Hexadecene (Fluka, Switzerland) and rapidly frozen in Balzers HPM 010 freezing machine working at 2200 bar. All cells were freeze substituted at -90°C in 2% OsO4 in absolute acetone. Serial sections cut to avoid misinterpretations due to the geometry of sections, were collected on carbon coated 100 mesh grids.


1985 ◽  
Vol 49 (5) ◽  
pp. 1365-1370 ◽  
Author(s):  
Hajimu Morioka ◽  
Masaru Ishihara ◽  
Misako Takezawa ◽  
Kazuo Hirayama ◽  
Eiichiro Suzuki ◽  
...  

1986 ◽  
Vol 10 (6) ◽  
pp. 651-658 ◽  
Author(s):  
Janine Patet ◽  
Joseph Huppert ◽  
Alain Fourcade ◽  
Haim Tapiero

1978 ◽  
Vol 75 (12) ◽  
pp. 5903-5907 ◽  
Author(s):  
R. F. Murphy ◽  
R. B. Wallace ◽  
J. Bonner

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