Genome variation of 5 clinical adenovirus type 2 isolates determined by restriction enzyme digestion analysis

1986 ◽  
Vol 87 (3-4) ◽  
pp. 321-326 ◽  
Author(s):  
E. Sandra McFarlane ◽  
J. H. B. Stewart ◽  
Hana James
1998 ◽  
Vol 36 (1) ◽  
pp. 15-19 ◽  
Author(s):  
Jacque J. Staats ◽  
Brandon L. Plattner ◽  
Jerome Nietfeld ◽  
Steve Dritz ◽  
M. M. Chengappa

Nineteen Streptococcus suis type 2 isolates were evaluated for their virulence in pigs and mice. Of these, seven were determined to be highly virulent in pigs on the basis of clinical sign scores and gross pathology and histopathology results. Clinical sign scores correlated with gross pathology and histopathology scores atP equal to 0.004 and P equal to 0.009, respectively. The virulence of highly virulent isolates in pigs compared somewhat with virulence in mice, but the correlation was not significant. No correlation of virulence was noted among the moderately virulent and avirulent isolates in pigs and mice. Chromosomal DNAs from all S. suis isolates were evaluated by PstI,PvuII, EcoRI, and HaeIII restriction enzyme digestion followed by hybridization with a digoxigenin-11-dUTP-labeled cDNA probe transcribed from 16S and 23S rRNAs from Escherichia coli. The hybridization patterns (ribotypes) varied depending upon the enzyme used, but a significant number of isolates determined to be highly virulent in pigs had unique hybridization patterns compared with those of the moderately virulent and avirulent isolates (P = 0.002). In addition, hemolysin activity showed a high correlation to virulence (P = 0.00008) and ribotype (P = 0.002).


1979 ◽  
Vol 30 (2) ◽  
pp. 462-471 ◽  
Author(s):  
M A Mirza ◽  
J Weber

1975 ◽  
Vol 16 (4) ◽  
pp. 767-774 ◽  
Author(s):  
R Schilling ◽  
B Weingärtner ◽  
E L Winnacker

1975 ◽  
Vol 16 (4) ◽  
pp. 759-766 ◽  
Author(s):  
A Tolun ◽  
U Pettersson

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