Cytochalasin D reduces osteoclastic bone resorption by inhibiting development of ruffled border-clear zone complex

1993 ◽  
Vol 53 (3) ◽  
pp. 217-221 ◽  
Author(s):  
Takahisa Sasaki ◽  
Kazuhiro Debari ◽  
Nobuyuki Udagawa
Keyword(s):  
Bone Resorption ◽  
Cytochalasin D ◽  
Osteoclastic Bone Resorption ◽  
Clear Zone ◽  
Ruffled Border

scholarly journals THE EFFECTS OF PARATHYROID HORMONE, COLCHICINE, AND CALCITONIN ON THE ULTRASTRUCTURE AND THE ACTIVITY OF OSTEOCLASTS IN ORGAN CULTURE

1974 ◽  
Vol 60 (2) ◽  
pp. 346-355 ◽  
Author(s):  
Marijke E. Holtrop ◽  
Lawrence G. Raisz ◽  
Hollis A. Simmons
Keyword(s):  
Parathyroid Hormone ◽  
Bone Resorption ◽  
Cross Sections ◽  
Clear Zone ◽  
Border Area ◽  
Fetal Rat ◽  
Calcitonin Treatment ◽  
Ruffled Border ◽  
45Ca Release ◽  
Massive Accumulation

The ultrastructure of osteoclasts was examined in fetal rat bones after stimulation or inhibition of resorption in culture. A central ruffled border area completely encircled by a clear zone was considered to represent the resorbing system of the cell. The proportion of ruffled border and clear zone in osteoclast cross sections was compared with changes in bone resorption as measured by the release of previously incorporated radioactive calcium (45Ca). In control cultures 55% of the osteoclast cross sections showed an area closely apposed to bone and this consisted mainly of clear zone; only 11% showed ruffled borders. Treatment with parathyroid hormone (PTH) increased 45Ca release, increased the frequency of finding areas closely apposed to bone (79%), and markedly increased the frequency of the ruffled border area (64%). Colchicine given concurrently with PTH decreased the number of osteoclasts. Colchicine or calcitonin treatment after PTH stimulation decreased the proportion of ruffled border area significantly by 1 h; this was followed by a decrease in 45Ca release. These inhibited osteoclasts resembled osteoclasts from control, unstimulated cultures, suggesting that the cells had returned to their inactive state. Colchicine-treated osteoclasts also showed a loss of microtubules and a massive accumulation of 100 Å filaments, suggesting that synthesis of microtubular subunits had increased.

Clear zone in osteoclast function: role of podosomes in regulation of bone-resorbing activity

1991 ◽  
Vol 261 (1) ◽  
pp. C1-C7 ◽  
Author(s):  
A. Teti ◽  
P. C. Marchisio ◽  
A. Z. Zallone
Keyword(s):  
Bone Resorption ◽  
Bone Matrix ◽  
Focal Adhesions ◽  
Cell Polarization ◽  
Free Calcium ◽  
Clear Zone ◽  
Discrete Structures ◽  
Free Calcium Concentration ◽  
Ruffled Border ◽  
Osteoclast Function

The adhesion of osteoclasts to the bone matrix is mandatory for bone resorption. Contact of the osteoclast with bone surface induces, in fact, cell polarization and organization of the resorbing apparatus, the so-called “ruffled border.” Cell-matrix interaction in osteoclasts is a complex phenomenon resulting from formation of the “clear zone,” a cytoplasmic area presenting the adhering plasma membrane, or “sealing membrane.” The sealing membrane surrounds the ruffled border and seals the resorbing compartment, namely the extracellular space in which bone resorption takes place. Adhesion at this level occurs via specialized discrete structures, the “podosomes.” Podosomes present most of the protein commonly found in focal adhesions, but with a peculiar organization. They are dynamic elements suitable for regulation, according with the functional demand of the cell. Their assembly increases during bone resorption and is regulated by the cytosolic free calcium concentration and the activity of protein kinase C.

Characterization of a Cell Line Derived From a Human Giant Cell Tumor That Stimulates Osteoclastic Bone Resorption

1993 ◽  
Vol &NA; (296) ◽  
pp. 229???241 ◽  
Author(s):  
RICHARD O. C. OREFFO ◽  
G. JUNE MARSHALL ◽  
MARY KIRCHEN ◽  
CARLOS GARCIA ◽  
WOLF E. GALLWITZ ◽  
...  
Keyword(s):  
Bone Resorption ◽  
Giant Cell Tumor ◽  
Cell Line ◽  
Giant Cell ◽  
Cell Tumor ◽  
Osteoclastic Bone Resorption ◽  
A Cell

Minocycline to be used a potential anti-bone resorption agents due to the suppression of osteoclastic bone resorption

2013 ◽  
Vol 55 (1) ◽  
pp. 16-22 ◽  
Author(s):  
Nobuyuki Udagawa ◽  
Masanori Koide ◽  
Midori Nakamura ◽  
Naoyuki Takahashi
Keyword(s):  
Bone Resorption ◽  
Osteoclastic Bone Resorption

α-Tocotrienol inhibits osteoclastic bone resorption by suppressing RANKL expression and signaling and bone resorbing activity

2011 ◽  
Vol 406 (4) ◽  
pp. 546-551 ◽  
Author(s):  
Hyunil Ha ◽  
Jong-Ho Lee ◽  
Ha-Neui Kim ◽  
Zang Hee Lee
Keyword(s):  
Bone Resorption ◽  
Osteoclastic Bone Resorption ◽  
Rankl Expression ◽  
Bone Resorbing Activity

Interleukin 1 (IL1) induces osteoblastic cells to stimulate osteoclastic bone resorption

Bone ◽  
1985 ◽  
Vol 6 (4) ◽  
pp. 276-276
Author(s):  
B.M. Thomson ◽  
T.J. Chambers
Keyword(s):  
Bone Resorption ◽  
Interleukin 1 ◽  
Osteoclastic Bone Resorption ◽  
Osteoblastic Cells

Simplified Approach to Enzymatic Identification of Osteoclastic Bone Resorption

1991 ◽  
Vol 14 (2) ◽  
pp. 81-83 ◽  
Author(s):  
Ian H. Parkinson ◽  
Nicola L. Fazzalari ◽  
Timothy C. Durbridge ◽  
Robert J. Moore
Keyword(s):  
Bone Resorption ◽  
Osteoclastic Bone Resorption ◽  
Simplified Approach

scholarly journals Bone Remodeling and the Role of TRAF3 in Osteoclastic Bone Resorption

2018 ◽  
Vol 9 ◽  
Author(s):  
Brendan F. Boyce ◽  
Jinbo Li ◽  
Lianping Xing ◽  
Zhenqiang Yao
Keyword(s):  
Bone Resorption ◽  
Bone Remodeling ◽  
Osteoclastic Bone Resorption

scholarly journals Cytoplasmic Terminus of Vacuolar Type Proton Pump Accessory Subunit Ac45 Is Required for Proper Interaction with V0Domain Subunits and Efficient Osteoclastic Bone Resorption

2008 ◽  
Vol 283 (19) ◽  
pp. 13194-13204 ◽  
Author(s):  
Haotian Feng ◽  
Taksum Cheng ◽  
Nathan J. Pavlos ◽  
Kirk H. M. Yip ◽  
Amerigo Carrello ◽  
...  
Keyword(s):  
Bone Resorption ◽  
Proton Pump ◽  
Osteoclastic Bone Resorption ◽  
Accessory Subunit

Possible involvement of focal adhesion kinase, p125FAK, in osteoclastic bone resorption

1995 ◽  
Vol 58 (4) ◽  
pp. 424-435 ◽  
Author(s):  
Sakae Tanaka ◽  
Naoyuki Takahashi ◽  
Nobuyuki Udagawa ◽  
Hiroshi Murakami ◽  
Ichiro Nakamura ◽  
...  
Keyword(s):  
Bone Resorption ◽  
Focal Adhesion Kinase ◽  
Focal Adhesion ◽  
Osteoclastic Bone Resorption
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